The antioxidant properties of plant flavonoids: their exploitation by molecular plant breeding

D’Amelia, Vincenzo; Aversano, Riccardo; Chiaiese, Pasquale; Carputo, Domenico

doi:10.1007/s11101-018-9568-y

Cited by 106 publications

(72 citation statements)

References 138 publications

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“…2a) and SchWRKYs (Fig. 2b) revealed their dynamic and differential expression in various tissues and that the range of expression 67, 81, 84_like, 85, 86, 88 and 89) showed very low or undetectable expression (FPKM values from 0 to 0.5) in all studied tissues, while 16 (20.2%) genes (18,23,03,48,87,08,11,47,79,10,51,45, 05, 06, 12 and 49) were highly expressed (FPKM > 5) in all tissues. Some of the remaining genes showed tissue specificities.…”

Section: Expression Patterns Of Wrky Genes In S Commersonii and S Cmentioning

confidence: 95%

“…From our analyses, interesting observations and different clues for future functional studies have emerged. For example, ScWRKY002 could be in some way involved in anthocyanin activation in flowers of S. commersonii: it interacts with anthocyanin bHLHs and the flower of this wild species strongly accumulates anthocyanins 14,67 . Previous studies reported that some WRKYs can be involved in the coordination of multiple biological processes.…”

Section: Identification Of Tissue-specific and Stress Responsive Wrkymentioning

confidence: 99%

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WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species

Villano

Esposito

D’Amelia

et al. 2020

Sci Rep

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Wild potatoes, as dynamic resource adapted to various environmental conditions, represent a powerful and informative reservoir of genes useful for breeding efforts. WRKY transcription factors (TFs) are encoded by one of the largest families in plants and are involved in several biological processes such as growth and development, signal transduction, and plant defence against stress. In this study, 79 and 84 genes encoding putative WRKY TFs have been identified in two wild potato relatives, Solanum commersonii and S. chacoense. Phylogenetic analysis of WRKY proteins divided ScWRKYs and SchWRKYs into three Groups and seven subGroups. Structural and phylogenetic comparative analyses suggested an interspecific variability of WRKYs. Analysis of gene expression profiles in different tissues and under various stresses allowed to select ScWRKY045 as a good candidate in woundingresponse, ScWRKY055 as a bacterial infection triggered WRKY and ScWRKY023 as a multiple stressresponsive WRKY gene. Those WRKYs were further studied through interactome analysis allowing the identification of potential co-expression relationships between ScWRKYs/SchWRKYs and genes of various pathways. Overall, this study enabled the discrimination of WRKY genes that could be considered as potential candidates in both breeding programs and functional studies.Plants experience environmental constrains and pathogen attacks during their life. Being sessile organisms, their survival depends on the ability to properly and promptly reprogram cellular networks. Several and different classes of transcription factors (TFs) work as "master regulators" and "selector genes", being able to control processes that specify cell types and developmental patterning and modulate specific pathways. Among them, WRKY factors are drawing a great deal of interest in the scientific community due to their ability to simultaneously cope with multiple stresses 1,2 . They are notorious for coordinating signals in plant immunity response against several pathogens and pest attacks 3,4 . More recently, it has been confirmed that WRKYs also base defence mechanism to abiotic stresses and play a key role in cross-talk pathway networks between plant response and development 5,6 . Their involvement into multiple stress response and in plant growth regulation is evidenced by their W-box specific DNA binding 7,8 . Besides, WRKY binds sugar responsive elements and, very recently, it has been demonstrated that they activate sugar responsive genes through an epigenetic mechanism of control 9 . The systematic classification of components of the WRKY family is well organized. It is based on the WRKY binding domain (WD) characteristics along with those of the Zinc Finger (ZF) motif, which is typically present downstream the WD. WD consists of 60 amino acids structured as four-stranded β-sheets able to enter the major groove of B-form DNA. The highly conserved motif is "WRKYGQK". According to the number of WDs and the type of zinc finger motif, WRKY proteins can be classified into three Groups,...

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Section: Expression Patterns Of Wrky Genes In S Commersonii and S Cmentioning

confidence: 95%

Section: Identification Of Tissue-specific and Stress Responsive Wrkymentioning

confidence: 99%

WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species

Villano

Esposito

D’Amelia

et al. 2020

Sci Rep

Self Cite

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“…Flavonoids can protect plants against various types of damage including that induced by ultraviolet (UV) irradiation, freezing, drought, and nitrogen deficiency [1]. Flavonoids are also thought to be important dietary ingredients and have also been intensively studied for their health benefits to humans and animals [7,8]. Flavonoids produced by plants have diverse biological activities, including anti-inflammatory [9], antioxidant [10], and anti-cancer activities [11].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Flavonoid 3’/5’/7-O-Methyltransferase from Citrus reticulata and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products

et al. 2020

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O-methylation of flavonoids is an important modification reaction that occurs in plants. O-methylation contributes to the structural diversity of flavonoids, which have several biological and pharmacological functions. In this study, an O-methyltransferase gene (CrOMT2) was isolated from the fruit peel of Citrus reticulata, which encoding a multifunctional O-methyltransferase and could effectively catalyze the methylation of 3’-, 5’-, and 7-OH of flavonoids with vicinal hydroxyl substitutions. Substrate preference assays indicated that this recombinant enzyme favored polymethoxylated flavones (PMF)-type substrates in vitro, thereby providing biochemical evidence for the potential role of the enzyme in plants. Additionally, the cytotoxicity of the methylated products from the enzymatic catalytic reaction was evaluated in vitro using human gastric cell lines SGC-7901 and BGC-823. The results showed that the in vitro cytotoxicity of the flavonoids with the unsaturated C2-C3 bond was increased after being methylated at position 3’. These combined results provide biochemical insight regarding CrOMT2 in vitro and indicate the in vitro cytotoxicity of the products methylated by its catalytic reaction.

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“…Cu(II) may lead to autoxidation of vitamin C, but some flavonoids, including quercetin, morin, and catechin exerted a protective effect in vitro against such outcome [31]. This reaction can be mainly underlined by a strong scavenger activity towards ROS and reactive nitrogen species, chelating metals involved in free radical generation and activation of antioxidant enzymes (reviewed in [86]).…”

Section: Molecular Studiesmentioning

confidence: 99%

Pro- and Antioxidant Effects of Vitamin C in Cancer in correspondence to Its Dietary and Pharmacological Concentrations

Pawłowska

Szczepańska

Błasiak

2019

Oxidative Medicine and Cellular Longevity

104

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Vitamin C is an antioxidant that may scavenge reactive oxygen species preventing DNA damage and other effects important in cancer transformation. Dietary vitamin C from natural sources is taken with other compounds affecting its bioavailability and biological effects. High pharmacological doses of vitamin C may induce prooxidant effects, detrimental for cancer cells. An oxidized form of vitamin C, dehydroascorbate, is transported through glucose transporters, and cancer cells switch from oxidative phosphorylation to glycolysis in energy production so an excess of vitamin C may limit glucose transport and ATP production resulting in energetic crisis and cell death. Vitamin C may change the metabolomic and epigenetic profiles of cancer cells, and activation of ten-eleven translocation (TET) proteins and downregulation of pluripotency factors by the vitamin may eradicate cancer stem cells. Metastasis, the main reason of cancer-related deaths, requires breakage of anatomical barriers containing collagen, whose synthesis is promoted by vitamin C. Vitamin C induces degradation of hypoxia-inducible factor, HIF-1, essential for the survival of tumor cells in hypoxic conditions. Dietary vitamin C may stimulate the immune system through activation of NK and T cells and monocytes. Pharmacological doses of vitamin C may inhibit cancer transformation in several pathways, but further studies are needed to address both mechanistic and clinical aspects of this effect.

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The antioxidant properties of plant flavonoids: their exploitation by molecular plant breeding

Cited by 106 publications

References 138 publications

WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species

WRKY genes family study reveals tissue-specific and stress-responsive TFs in wild potato species

Characterization of a Flavonoid 3’/5’/7-O-Methyltransferase from Citrus reticulata and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products

Pro- and Antioxidant Effects of Vitamin C in Cancer in correspondence to Its Dietary and Pharmacological Concentrations

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