The Antioxidant Potential of Ficus Umbellata Vahl (Moraceae) That Accelerates In Vitro and the In Vivo Anti-Inflammatory Protective Effects

Silihe, Kevine Kamga; Zingue, Stéphane; Sipping, Marius Trésor Kemegne; Cazanevscaia, Anna Busuioc; Botezatu, Andreea Veronica Dediu; Njamen, Dieudonné; Dinică, Rodica Mihaela

doi:10.3390/app12189070

Cited by 4 publications

(6 citation statements)

References 44 publications

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“…The increased production of ROS triggers a cascade of reactions leading to the onset of an inflammatory response that can be chronic and lead to diseases such as cancer [ 57 ]. This inflammatory response is closely associated with all stages of malignant development and progression of most types of cancer, as well as with the efficacy of cancer therapies [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Duguetia confinis Engl. & Diels (Annonaceae) inhibitory and cytotoxic effects on breast adenocarcinoma growth both in vitro and in vivo

Nkuimi,

Silihe,

Tabi

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Duguetia confinis Engl. & Diels (Annonaceae) inhibitory and cytotoxic effects on breast adenocarcinoma growth both in vitro and in vivo

Nkuimi,

Silihe,

Tabi

et al. 2024

Heliyon

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“…The assessment of the total antioxidant capacity of M. arboreus extracts demonstrated a superior capacity in the methanolic extract compared to the aqueous extract. Additionally, it is established that the total polyphenol content exhibits a robust correlation with free radical scavenging activities [20]. Likewise, prior investigations into distinct components of M. arboreus (leaves, bark, and roots) underscored the abundance of phenolic compounds in the tested extracts, along with their antiradical activities [58][59][60].…”

Section: Discussionmentioning

confidence: 99%

“…The free radical scavenging capacity of M. arboreus extracts was tested by the DPPH radical assay as described by Silihe et al [20], with a few modifications. Briefly, in a 96-well microplate, 100 µl of sample was introduced and serially diluted from an initial concentration of 1.25 mg/ml.…”

Section: Dpph Scavenging Activitymentioning

confidence: 99%

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2024

SM J Urol

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Benign prostatic hyperplasia (BPH) is one of the common disorders amongst the geriatric male population, affecting their quality of life. Since conventional drugs used for the treatment are sometimes accompanied by serious side effects, the search for alternative treatments remains urgent. This study aimed to evaluate the antioxidant activity and the protective effects of the aqueous and methanolic extracts of Myrianthus arboreus (M. arboreus) on a BPH model induced in male Wistar rats. The antioxidant activities of the extracts were assayed in vitro by free radical scavenging using DPPH and ABTS assays, total antioxidant capacity. For the in vivo study, rats were randomly divided into nine groups of six rats each. Groups 1 and 2 received distilled water, group 3 received finasteride (5 mg/kg), while groups 4 to 9 received 50, 100, and 200 mg/kg of M. arboreus respectively. The treatment lasted 28 days after which, animals were sacrificed. Relative organ weights, histopathological changes, total protein levels, and oxidative status were determined. In addition, some pro-inflammatory cytokines and prostate-specific antigen levels were measured. Both extracts of M. arboreus demonstrated strong radical scavenging and total antioxidant capacity in vitro. They counteracted the effects of testosterone by decreasing the relative weight and volume of the prostate, the prostate epithelium height, and the total protein level in the prostate. Likewise, they improved the oxidative status and reduced the concentrations of cytokines in the prostate, and the serum PSA level. M. arboreus extracts contain bioactive compounds with antioxidant properties which confers protective abilities against the occurrence of BPH.

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“…Erythrocytes are well equipped to degrade reactive oxygen species via the actions of superoxide dismutase that converts O2 into H2O2, which is further catabolized by catalase and glutathione peroxidase [20]. As hemolysis is the cytotoxicity indicator, hemolytic assays are performed to check the hemolytic effect of compounds before establishing their pharmacological preparations [29]. In the current study, erythrocytes are used as a model system for studying oxidative damage induced by HCl and its pathophysiology.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Protective Effect of Leaf Extract of Ficus Deltoidea Jack (Moraceae) on Biomarkers of Oxidative Stress in the Human Erythrocytes

Tkachenko¹,

Kurhaluk²,

Buyun³

et al. 2023

BHT

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Purpose: to investigate the antioxidant properties of the aqueous extracts derived from leaves of Ficus deltoidea using the model of human blood. For this purpose, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), content of aldehydic and ketonic derivatives of oxidatively modified proteins, total antioxidant capacity (TAC)] in the human erythrocytes after in vitro incubation with aqueous extracts derived from leaves of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL) were used. Resistance of human erythrocytes after in vitro treatment by aqueous extracts derived from leaves of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL) was evaluated by HCl-induced hemolysis using a percentage of hemolyzed erythrocytes per each 30 sec. and a total time of hemolysis. Methodology. The leaves of F. deltoidea were collected in M.M. Gryshko National Botanic Garden (Kyiv, Ukraine). Blood (10-20 mL) was obtained from normal volunteers via venipuncture (4 males and 5 females aged 28-53 years old). An erythrocyte suspension at 1% hematocrit was incubated with 4 mM phosphate buffer (pH 7.4) (control) and pre-incubated with the extract of F. deltoidea (at a final concentration of 5 mg/mL and 0.5 mg/mL, respectively) at 37 °C for 60 min. The level of lipid peroxidation was determined by quantifying the concentration of 2-thiobarbituric acid reacting substances (TBARS). The rate of protein oxidative destruction was estimated from the reaction of the resultant carbonyl derivatives of amino acid reaction with 2,4-dinitrophenylhydrazine (DNFH). The total antioxidant capacity (TAC) in the samples was estimated by measuring the TBARS level after Tween 80 oxidation. The HCl-induced resistance of erythrocytes was measured spectrophotometrically with 0.1M HCl. The significance of differences (significance level, p < 0.05) was examined using the Mann-Whitney U test. Scientific novelty. The treatment of human erythrocytes by extract derived from leaves of F. deltoidea at the final concentration of 5 mg/mL resulted in an increase of TBARS as biomarkers of lipid peroxidation and aldehydic derivatives of oxidatively modified proteins with a simultaneous decrease in the total antioxidant capacity compared to the untreated samples. On the other hand, in vitro treatment of human erythrocytes with an extract derived from leaves of F. deltoidea at the final concentration of 0.5 mg/mL resulted in the same values of TBARS level, aldehydic and ketonic derivatives of OMP, and total antioxidant capacity as the untreated samples. Extract of F. deltoidea at the final concentrations of 5 mg/mL and 0.5 mg/mL possess hemolytic properties to human erythrocyte suspension after 1-h incubation in vitro. Conclusions. The changes in the oxidative stress biomarkers using the in vitro model of human erythrocytes to evaluate the antioxidant activities of the aqueous extract derived from the leaves of F. deltoidea at the two final concentrations (5 mg/mL and 0.5 mg/mL) revealed that high concentration of extract (5 mg/mL) resulted in the increase of lipid peroxidation and protein oxidation with a simultaneous decline in the total antioxidant capacity. HCl-induced hemolysis was activated after the treatment by extract derived from the leaves of F. deltoidea at the two final concentrations. More studies are warranted in the future, to illustrate the potential and mechanisms of F. deltoidea in preventing oxidative stress using different cell models in vitro and different final concentrations of the extract. Also, further studies are warranted to identify the bioactive components that contribute to this protective effect.

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The Antioxidant Potential of Ficus Umbellata Vahl (Moraceae) That Accelerates In Vitro and the In Vivo Anti-Inflammatory Protective Effects

Cited by 4 publications

References 44 publications

Duguetia confinis Engl. & Diels (Annonaceae) inhibitory and cytotoxic effects on breast adenocarcinoma growth both in vitro and in vivo

Duguetia confinis Engl. & Diels (Annonaceae) inhibitory and cytotoxic effects on breast adenocarcinoma growth both in vitro and in vivo

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In Vitro Protective Effect of Leaf Extract of Ficus Deltoidea Jack (Moraceae) on Biomarkers of Oxidative Stress in the Human Erythrocytes

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