The antioxidant effect of Echinacea angustifolia and Echinacea purpurea in rat colitis model induced by acetic acid

Abstract

Abstract:Background: Ulcerative colitis is a chronic infl ammatory condition of the colon, and reactive oxidative metabolites (ROMs) play an important role in its pathogenesis. Alternative therapies such as herbal remedies are increasingly being used in the treatment of ulcerative colitis for better clinical outcome of ulcerative colitis and less adverse effects. Echinacea has many features including antioxidant and wound-healing properties. Hence, the present study was undertaken to evaluate the protective effect of Echinacea spp. on experimental colitis model induced by acetic acid in Wistar albino rats. Methods: Acute colitis was induced by intrarectal administration of acetic acid. Rats were divided into four groups, namely control, Echinacea-administered, Echinacea-administered-colitis and colitis. Malondialdehyde and total antioxidant status were assayed in tissue samples. Histopathological evaluation was also performed. Results: Macroscopic and microscopic scores were signifi cantly higher in colitis group compared to control, Echinacea and Echinacea-colitis groups (p < 0.001). There was no signifi cant differences in respect of macroscopic and microscopic scores between control, Echinacea and Echinacea-colitis groups (p > 0.3, p > 0.22). Malondialdehyde levels were elevated in colitis group compared to other groups (p < 0.001). Total antioxidant status was signifi cantly higher in Echinacea group compared with other groups and also signifi cantly higher in Echinacea-colitis group compared with colitis group (p < 0.001, p < 0.001, respectively). Conclusion: Echinacea may possibly have some therapeutic usefulness in the management of ulcerative colitis (Tab. 2, Fig. 4, Ref. 35). Text in PDF www.elis.sk.

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“…This is consistent with previous data observed in rats (Dogan et al, 2014) and mice (Somani et al, 2014). Indeed, during intestinal inflammation, among the mechanisms used by phagocytes is the production of reactive oxygen species starting from superoxide anions (O2 ·-) (Gougerot-Pocidalo et al, 2002).…”

The Effect of N. Acetylcysteine and Ginger on Acetic Acid Induced Colitis in Adult Male Albino Rat: Histological, Immunohistochemical and Morphometric Study

“…The procedure was performed by injecting 0.5 ml of 4% acetic acid into the colon followed by injection of 2 ml air to ensure adequate spreading of the acetic acid inside the colon. The rats were maintained in a head down position for 5 minutes to prevent leakage [32].…”

Phytopharmacologic preparations as predictors of plant bioactivity: A particular approach to Echinacea purpurea (L.) Moench antioxidant properties

“…On the other hand, Dogan et al in a colite-induced model, described a mucosal protective effect, mainly conferred by the Echinacea antioxidative constituents, caffeic acid and echinacoside [19].…”

“…Indeed, not only environmental and alimentary patterns acts as main triggering factors, but also free radicals overproduction by organic metabolism plays an important role [23][24][25][26]. In spite of the human body possess proper detoxifying mechanisms, the lost of the organic homeostasis leads to several changes in metabolic pathways which consequently improves the oxidative stress, mainly characterized by lipid, protein and DNA modifications, and then the likelihood of diseases/disorders occurrence [27,19]. E. purpurea has been showing promissory antioxidant properties, most of them directly related with its richness in phenolic compounds, including flavonoids and phenolic acids [6,19,28].…”

“…In spite of the human body possess proper detoxifying mechanisms, the lost of the organic homeostasis leads to several changes in metabolic pathways which consequently improves the oxidative stress, mainly characterized by lipid, protein and DNA modifications, and then the likelihood of diseases/disorders occurrence [27,19]. E. purpurea has been showing promissory antioxidant properties, most of them directly related with its richness in phenolic compounds, including flavonoids and phenolic acids [6,19,28]. Furthermore, and despite availability of commercial products containing E. purpurea, their antioxidant properties should be determined and compared with the raw material and derived extracts, which was the main purpose of this pioneer study.…”