The antioxidant caffeic acid phenethyl ester induces apoptosis associated with selective scavenging of hydrogen peroxide in human leukemic HL-60 cells

Chen, Yu-Jen; Shiao, Ming‐Shi; Wang, Shengyuan

doi:10.1097/00001813-200102000-00008

Cited by 172 publications

(106 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CAPE is, in fact, an active component of propolis derived from the bark of conifer trees and carried by honeybees to their hives. The similarity to curcumin is striking because CAPE is also a Michael reaction acceptor that has a broad spectrum of biological activities, including anti-inflammatory (Natarajan et al, 1996;Michaluart et al, 1999), antioxidant (Chen et al, 2001), and anti-cancer effects (Frenkel et al, 1993;Huang et al, 1996). We report our finding that CAPE is a potent inducer of HO-1.…”

mentioning

confidence: 49%

Caffeic Acid Phenethyl Ester and Curcumin: A Novel Class of Heme Oxygenase-1 Inducers

Scapagnini

Foresti²,

Calabrese³

et al. 2002

Mol Pharmacol

291

210

View full text Add to dashboard Cite

Heme oxygenase-1 (HO-1) is a redox-sensitive inducible protein that provides efficient cytoprotection against oxidative stress. Curcumin, a polyphenolic natural compound that possesses anti-tumor and anti-inflammatory properties, has been reported recently to induce potently HO-1 expression in vascular endothelial cells (Free Rad Biol Med 28:1303-1312. Here, we extend our previous findings by showing that caffeic acid phenethyl ester (CAPE), another plant-derived phenolic agent, markedly increases heme oxygenase activity and HO-1 protein in astrocytes. The effect seems to be related to the peculiar chemical structures of curcumin and CAPE, because analogous antioxidants containing only portions of these two molecules were totally ineffective. At a final concentration of 30 M, both curcumin and CAPE maximally up-regulated heme oxygenase activity while promoting marked cytotoxicity at higher concentrations (50 -100 M). Similar results were obtained with Curcumin-95, a mixture of curcuminoids commonly used as a dietary supplement. Incubation of astrocytes with curcumin or CAPE at concentrations that promoted maximal heme oxygenase activity resulted in an early increase in reduced glutathione followed by a significant elevation in oxidized glutathione contents. A curcumin-mediated increase in heme oxygenase activity was not affected by the glutathione precursor and thiol donor N-acetyl-L-cysteine. These data suggest that regulation of HO-1 expression by polyphenolic compounds is evoked by a distinctive mechanism which is not necessarily linked to changes in glutathione but might depend on redox signals sustained by specific and targeted sulfydryl groups. This study identifies a novel class of natural substances that could be used for therapeutic purposes as potent inducers of HO-1 in the protection of tissues against inflammatory and neurodegenerative conditions. Heme oxygenase-1 (HO-1) is a ubiquitous and redox-sensitive inducible stress protein (Motterlini et al., 2002). In mammals, the crucial participation of HO-1 gene expression in alleviating organ dysfunction and counteracting metabolic disorders is supported by consistent reports showing a protective role for the products of the enzymatic activity of HO-1. Heme serves as a substrate for HO-1 in the formation of carbon monoxide, free ferrous iron, and biliverdin; the latter is rapidly converted to bilirubin by biliverdin reductase (Choi and Alam, 1996;. A substantial body of evidence demonstrates that increased carbon monoxide and bilirubin effectively contribute to modulate important physiological processes within the cardiovascular, immune, and nervous systems. These include the regulation of vessel tone (Motterlini et al., 1998), inhibition of platelet aggregation (Durante and Schafer, 1998), and prevention of cell death and tissue injury (Clark et al., 2000b). The overall concept emerging from these and other studies is that the induction of HO-1 is an essential step in the cellular adaptation to stress inflicted by pathological events.Apart from ...

show abstract

mentioning

confidence: 49%

Caffeic Acid Phenethyl Ester and Curcumin: A Novel Class of Heme Oxygenase-1 Inducers

Scapagnini

Foresti²,

Calabrese³

et al. 2002

Mol Pharmacol

291

210

View full text Add to dashboard Cite

show abstract

“…15,16) On the contrary, an interesting paper supporting a correlation between antioxidation activity and apoptosis has appeared. 17) However, further investigation remains to clarify whether the antioxidative eŠect of celastrol is associated with the apoptosis.…”

Section: )mentioning

confidence: 99%

Apoptosis Induction in HL-60 Cells and Inhibition of Topoisomerase II by Triterpene Celastrol

Nagase

Oto

Sugiyama

et al. 2003

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

“…Propolis has many biological effects such as anti-inflammatory, antifungal, antiviral, antioxidants [7][8][9][10][11][12], and wound healing [7,13] and has the ability to stop the proliferation of cancer cells [14] and controlling tumor growth and metastases [15].…”

Section: Introductionmentioning

confidence: 99%

An Intervention Airing the Effect of Iranian Propolis on Epithelial Dysplasia of the Tongue: A Preliminary Study

Salehi¹,

Mina

Moghadamnia

et al. 2017

JCDR

View full text Add to dashboard Cite

Introduction: Since oral cancer is one of the causes of mortality, the use of materials or methods that can reduce cancer or prevent its progression has particular importance.Aim: Aim of the study was to evaluate the antitumor effects of Iranian propolis on dysplastic changes of oral mucosa in rats. Materials and Methods:This study was performed on 28 Wistar male rats (aged 7-11 weeks, 160±20 g). They were divided into four groups of seven rats. The Group 1 received: 0.5% 7,12-Dimethylbenz[a]anthracene (DMBA), the Group 2: 0.5% DMBA and 100 mg/kg propolis, the Group 3: 0.5% DMBA and 200 mg/ kg propolis, and the Group 4: 0.5% DMBA and 400 mg/kg propolis. DMBA in all groups was administered topically (brush) and propolis was injected intraperitoneally. DMBA was brushed twice on the lingual dorsum three times a week for 20 weeks. Propolis injection just every other day and in the days after DMBA was administered for 20 weeks. Rats were sacrificed, and histological examinations were performed on tongue specimen.Results: Propolis can reduce the degree of dysplasia in doses 100 mg/kg, 200 mg/kg, and 400 mg/kg compared to control (Group 1) (p=0.017, p=0.02, and p=0.002, respectively). Conclusion:The results of this study showed propolis can dose-dependently prevent DMBA-induced dysplasia of the oral mucosa in animal model.

show abstract

The antioxidant caffeic acid phenethyl ester induces apoptosis associated with selective scavenging of hydrogen peroxide in human leukemic HL-60 cells

Cited by 172 publications

References 23 publications

Caffeic Acid Phenethyl Ester and Curcumin: A Novel Class of Heme Oxygenase-1 Inducers

Caffeic Acid Phenethyl Ester and Curcumin: A Novel Class of Heme Oxygenase-1 Inducers

Apoptosis Induction in HL-60 Cells and Inhibition of Topoisomerase II by Triterpene Celastrol

An Intervention Airing the Effect of Iranian Propolis on Epithelial Dysplasia of the Tongue: A Preliminary Study

Contact Info

Product

Resources

About