The antioxidant and antimicrobial properties of phenol-rich extracts of Dracocephalum forrestii W. W. Smith shoot cultures grown in the nutrient sprinkle bioreactor

Weremczuk-Jeżyna, Izabela; Kochan, Ewa; Szymczyk, Piotr; Lisiecki, Paweł; Kuźma, Łukasz; Grzegorczyk-Karolak, Izabela

doi:10.1016/j.phytol.2019.01.032

Cited by 18 publications

(15 citation statements)

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“…Similarly, the dicaffeoylquinic acids demonstrate antioxidant and antiviral activity, particularly against HIV integrase and HIV replication in tissue culture [ 49 , 50 , 51 , 52 ]. Chlorogenic acid and dicaffeoylquinic acid were previously detected in D. forrestii non-transformed shoots grown in an NSB; however, chlorogenic acid production was less than 5% of that found in transformed shoots grown in all tested bioreactors, and dicaffeoylquinic acid level was only present in low quality, as noted previously [ 28 ]. The compounds in transformed shoot cultured in bioreactors and on solid media were present in similar amounts [ 22 ].…”

Section: Resultssupporting

confidence: 79%

“…Slightly higher EC 50 values have previously been found in non-transformed D. forrestii shoots grown in the same bioreactor: 30.7 μg/mL and 69.9 μg/mL, respectively [ 37 ]. In addition, the FRAP results for transformed shoots of D. forrestii were 724.9 μM Fe(II)/g DW, half those of the non-transformed shoots, i.e., 1319 μM Fe(II)/g DW of extract [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

“…The strongest activity and lowest values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) (10 mg/mL) were found against Gram-positive bacteria strains Staphylococcus epidermidis and Bacillus cereus , and Gram-negative bacteria Escherichia coli ( Table 4 ). The non-transformed shoots of D. forrestii have been found to demonstrate slightly higher antibacterial potential against these bacteria [ 28 ]. However, even higher MIC values have been obtained from alcohol extracts of the aerial parts of other Dracocephalum species: Dracocephalum moldavica extract inhibited growth E. coli and Klebsiella pneumoniae in the range 10–40 mg/mL [ 67 ], and the MIC of the extract from Dracocephalum kotschyi against E. coli was 25–100 mg/mL.…”

Section: Resultsmentioning

confidence: 99%

“…Salman et al [ 70 ] reported that the essential oil of D. moldavica displays strong antifungal properties against Candida albicans (MIC < 1.56 μm/mL). Weremczuk-Jeżyna et al [ 28 ] report that non-transformed shoots of D. forrestii showed better antifungal effect against Candida strains (MIC = 2.5 mg/mL). This lower antimicrobial potential of transformed shoots may have been a result of the absence of salvianolic acid B in the extract, as was observed for antioxidant activity [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

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Transformed Shoots of Dracocephalum forrestii W.W. Smith from Different Bioreactor Systems as a Rich Source of Natural Phenolic Compounds

et al. 2020

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Transformed shoots of the Tibetan medicinal plant Dracocephalum forrestii were cultured in temporary immersion bioreactors (RITA and Plantform) and in nutrient sprinkle bioreactor (NSB) for 3 weeks in MS (Murashige and Skoog) liquid medium with 0.5 mg/L BPA (N-benzyl-9-(2-tetrahydropyranyl)-adenine) and 0.2 mg/L IAA (indole-3-acetic acid). The greatest biomass growth index (GI = 52.06 fresh weight (FW) and 55.67 dry weight (DW)) was observed for shoots in the RITA bioreactor, while the highest multiplication rate was found in the NSB (838 shoots per bioreactor). The levels of three phenolic acids and five flavonoid derivatives in the shoot hydromethanolic extract were evaluated using UHPLC (ultra-high performance liquid chromatography). The predominant metabolite was rosmarinic acid (RA)—the highest RA level (18.35 mg/g DW) and total evaluated phenol content (24.15 mg/g DW) were observed in shoots grown in NSB. The NSB culture, i.e., the most productive one, was evaluated for its antioxidant activity on the basis of reduction of ferric ions (ferric reducing antioxidant power, FRAP) and two scavenging radical (O2•– and DPPH, 1,1-diphenyl-2-picrylhydrazyl radical) assays; its antibacterial, antifungal, and antiproliative potential against L929 cells was also tested (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test). The plant material revealed moderate antioxidant and antimicrobial activities and demonstrated high safety in the MTT test—no cytotoxicity at concentrations up to 50 mg/mL was found, and less than a 20% decrease in L929 cell viability was observed at this concentration.

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Section: Resultssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Transformed Shoots of Dracocephalum forrestii W.W. Smith from Different Bioreactor Systems as a Rich Source of Natural Phenolic Compounds

et al. 2020

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“…Bioreactor production of SMs also depends on the pharmacological significance and health benefits for humans of the target SMs. Selected examples are the production of RA, and resveratrol, and an overview is presented in [38,40] from different plants, such as Ocinum basilicum [45], Salvia officinalis [40], Coleus blumei [38], and Dracocephalum forrestii [39] have been developed. Different types of bioreactors and strategies to enhance the RA accumulation, such as metabolic engineering and elicitation with abiotic/ biotic elicitors have been applied [18].…”

Section: The Production Of Plant Secondary Metabolites In Bioreactorsmentioning

confidence: 99%

Green (cell) factories for advanced production of plant secondary metabolites

Marchev

Yordanova

Georgiev

2020

Critical Reviews in Biotechnology

113

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For centuries plants have been intensively utilized as reliable sources of food, flavoring, agrochemical and pharmaceutical ingredients. However, plant natural habitats are being rapidly lost due to climate change and agriculture. Plant biotechnology offers a sustainable method for the bioproduction of plant secondary metabolites using plant in vitro systems. The unique structural features of plant-derived secondary metabolites, such as their safety profile, multi-target spectrum and "metabolite likeness," have led to the establishment of many plant-derived drugs, comprising approximately a quarter of all drugs approved by the Food and Drug Administration and/or European Medicinal Agency. However, there are still many challenges to overcome to enhance the production of these metabolites from plant in vitro systems and establish a sustainable large-scale biotechnological process. These challenges are due to the peculiarities of plant cell metabolism, the complexity of plant secondary metabolite pathways, and the correct selection of bioreactor systems and bioprocess optimization. In this review, we present an integrated overview of the possible avenues for enhancing the biosynthesis of high-value marketable molecules produced by plant in vitro systems. These include metabolic engineering and CRISPR/Cas9 technology for the regulation of plant metabolism through overexpression/repression of single or multiple structural genes or transcriptional factors. The use of NMR-based metabolomics for monitoring metabolite concentrations and additionally as a tool to study the dynamics of plant cell metabolism and nutritional management is discussed here. Different types of bioreactor systems, their modification and optimal process parameters for the labor industrial-scale production of plant secondary metabolites are specified.

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Medicinal Plants Domestication, Cultivation, Improvement, and Alternative Technologies for the Production of High Value Therapeutics: An Overview

Ramawat

Arora

2021

Sustainable Development and Biodiversity

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The antioxidant and antimicrobial properties of phenol-rich extracts of Dracocephalum forrestii W. W. Smith shoot cultures grown in the nutrient sprinkle bioreactor

Cited by 18 publications

References 51 publications

Transformed Shoots of Dracocephalum forrestii W.W. Smith from Different Bioreactor Systems as a Rich Source of Natural Phenolic Compounds

Transformed Shoots of Dracocephalum forrestii W.W. Smith from Different Bioreactor Systems as a Rich Source of Natural Phenolic Compounds

Green (cell) factories for advanced production of plant secondary metabolites

Medicinal Plants Domestication, Cultivation, Improvement, and Alternative Technologies for the Production of High Value Therapeutics: An Overview

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