The Antinociceptive Effect of the Combination of Spinal Morphine with Systemic Morphine or Buprenorphine

Nemirovsky, Anna; Chen, Lianhua; Zelman, Vladimir; Jurna, I.

doi:10.1097/00000539-200107000-00039

Cited by 63 publications

(44 citation statements)

References 10 publications

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“…Thus, these overall results suggest multiple action sites of PcL. Because the hot plate is a specific central antinociceptive test in which opioid agents exert their analgesic effects via supra spinal and spinal receptors, 28) and considering the results of this study, the hot plate test was selected to be used to evaluate a possible central antinociception via supra spinal and spinal receptors for the PcL analgesic effect. Although morphine caused a significant increase in the latency for mice licking the paw when compared to the control group, PcL did not significantly modify the latencies compared to the saline or indomethacine controls (Fig.…”

Section: Resultsmentioning

confidence: 99%

Antinociceptive and Anti-inflammatory Activities of Lectin from Marine Red Alga Pterocladiella capillacea

Silva

Lima

Araújo

et al. 2010

Biological & Pharmaceutical Bulletin

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Researchers see algae as a promising tool to discover both efficient and safe agents for pain therapy. We evaluated the antinociceptive and anti-inflammatory activities of lectin from the marine alga Pterocladiella capillacea lectin (PcL). PcL was purified and tested in classical models of nociception and inflammation. Male Swiss mice received PcL 30 min prior to receiving 0.8% acetic acid (10 m ml/10 g, i.p.), 1% formalin (20 m ml/intraplantar) or the hot plate test, and were compared to untreated animals or animals pretreated with indomethacin or morphine. PcL (0.9, 8.1 or 72.9 mg/kg, i.v.) significantly reduced the number of writhes (30%, 39%, and 52%, respectively). PcL (72.9 mg/kg, i.v.) also reduced (pϽ Ͻ0.05) both the first and second phases of the formalin test by 58% and 87%, respectively. However, PcL (72.9 mg/kg) did not present significant antinociceptive effects in the hot plate test when compared to morphine, suggesting that its antinociceptive action occurs via peripheral rather than a central-acting mechanism. It was also observed that leukocyte migration was induced by carrageenan (500 m mg/cavity) in male Wistar rats and that PcL (8.1 mg/kg, i.v.) significantly reduced neutrophil migration by 84%, as compared to untreated animals, suggesting inhibition of inflammatory mediators. The data indicated that PcL has peripheral actions with both anti-inflammatory and antinociceptive properties.

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Section: Resultsmentioning

confidence: 99%

Antinociceptive and Anti-inflammatory Activities of Lectin from Marine Red Alga Pterocladiella capillacea

Silva

Lima

Araújo

et al. 2010

Biological & Pharmaceutical Bulletin

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“…29,30 The antinociceptive effect of V. ashei extract was investigated by the standard Eddy's hot plate method 19 as a thermal pain experimental model. 31,32 Analysis of the data revealed a statistically significant difference in the hot plate reaction time between the test and the control groups (P < .05). Thus V. ashei exhibited a significant analgesic effect at the doses studied after 1 hour and after 21 days of administration.…”

Section: Discussionmentioning

confidence: 96%

Preliminary Studies on the Antinociceptive Activity of Vaccinium ashei Berry in Experimental Animal Models

Ramirez

Guterres

Dickel

et al. 2010

Journal of Medicinal Food

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The aim of this study was to carry out pharmacological screening in order to evaluate the potential effects of lyophilized fruits of different cultivars of Vaccinium ashei Reade (Family Ericaceae) berries, commonly known as rabbiteye blueberries, on nociception. This was achieved using the formalin, hot plate, tail-flick, and writhing tests in mice. During this experiment the mice consumed approximately 3.2-6.4 mg=kg=day (p.o.) of the anthocyanins. The extract was administered for 21 days or 60 minutes before test. Morphine and diclofenac (10 mg=kg, p.o.) as the standard drug (positive control) and water (via oral gavage) as the negative control were administered before all tests. The blueberry extract produced a significant decrease in constrictions induced by acetic acid and caused graded inhibition of the second phase of formalin-induced pain. Moreover, in both the hot plate and tail-flick tests, it significantly increased the threshold. These data suggest that the extract from V. ashei produced antinociceptive effects, as demonstrated in the experimental models of nociception in mice. Additional experiments are necessary in order to clarify the true target for the antinociceptive effects of rabbiteye blueberry extract.

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“…Because the hot plate and tail flick tests both measure responses to thermal pain and often reflect central drug actions mediated by supraspinal and spinal mechanisms [26,27] , our results suggested that the antinociceptive effects of MB-1C-OH may occur via a peripheral-rather than a central-acting mechanism. We further evaluated the antinociception produced by MB-1C-OH using the acetic acid-induced writhing and formalin tests.…”

Section: Discussionmentioning

confidence: 83%

Novel κ-opioid receptor agonist MB-1C-OH produces potent analgesia with less depression and sedation

et al. 2015

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Aim: To characterize the pharmacological profiles of a novel κ-opioid receptor agonist MB-1C-OH. Methods: [ 3 H]diprenorphine binding and [ 35 S]GTPγS binding assays were performed to determine the agonistic properties of MB-1C-OH. Hot plate, tail flick, acetic acid-induced writhing, and formalin tests were conducted in mice to evaluate the antinociceptive actions. Forced swimming and rotarod tests of mice were used to assess the sedation and depression actions. Results: In [ 3 H]diprenorphine binding assay, MB-1C-OH did not bind to μ-and δ-opioid receptors at the concentration of 100 μmol/L, but showed a high affinity for κ-opioid receptor (K i =35 nmol/L). In [ 35 S]GTPγS binding assay, the compound had an E max of 98% and an EC 50 of 16.7 nmol/L for κ-opioid receptor. Subcutaneous injection of MB-1C-OH had no effects in both hot plate and tail flick tests, but produced potent antinociception in the acetic acid-induced writhing test (ED 50 =0.39 mg/kg), which was antagonized by pretreatment with a selective κ-opioid receptor antagonist Nor-BNI. In the formalin test, subcutaneous injection of MB-1C-OH did not affect the flinching behavior in the first phase, but significantly inhibited that in the second phase (ED 50 =0.87 mg/kg). In addition, the sedation or depression actions of MB-1C-OH were about 3-fold weaker than those of the classical κ agonist (-)U50,488H. Conclusion: MB-1C-OH is a novel κ-opioid receptor agonist that produces potent antinociception causing less sedation and depression.

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The Antinociceptive Effect of the Combination of Spinal Morphine with Systemic Morphine or Buprenorphine

Cited by 63 publications

References 10 publications

Antinociceptive and Anti-inflammatory Activities of Lectin from Marine Red Alga Pterocladiella capillacea

Antinociceptive and Anti-inflammatory Activities of Lectin from Marine Red Alga Pterocladiella capillacea

Preliminary Studies on the Antinociceptive Activity of Vaccinium ashei Berry in Experimental Animal Models

Novel κ-opioid receptor agonist MB-1C-OH produces potent analgesia with less depression and sedation

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