The antimicrobial properties of light-activated polymers containing methylene blue and gold nanoparticles

Perni, Stefano; Piccirillo, Clara; Pratten, Jonathan; Prokopovich, Polina; Chrzanowski, Wojciech; Parkin, Ivan P.; Wilson, Michael

doi:10.1016/j.biomaterials.2008.09.020

Cited by 238 publications

(249 citation statements)

References 12 publications

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“…Apparently, the decrease of the swelling ratio in the composites does not affect the MB release profile. Also it appears that the Au NPs do not limit the release of MB from the gelatin matrix, conversely to the effect reported for the MB release from Au nanocomposites prepared with silicone elastomers [35] and κ-carrageenan [36]. The decrease of MB release in these composites was ascribe to the interaction between Au NPs and MB, most probably by chemisorption of MB onto Au NPs surface via sulfur atoms [37], that limits the diffusion of MB from the polymer matrix.…”

Section: In Vitro Mb Releasementioning

confidence: 67%

Effects of Au nanoparticles on thermoresponsive genipin-crosslinked gelatin hydrogels

2013

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Gold gelatin hydrogel nanocomposites crosslinked with genipin have been prepared, and the effect of citrate capped Au nanoparticles (NPs) as nanofillers in the crosslinking and swelling of gelatin and release of a model drug (methylene blue) from gelatin nanocomposites have been investigated. The citrate-capped Au NPs prevented the crosslinking reaction between the gelatin and genipin and resulted in less crosslinked hydrogels. Although less crosslinked, the Au gelatin nanocomposites swelled less than the unfilled crosslinked gelatin. The gelatin composites were optically active and thermo-sensitive in a temperature range acceptable for living cells. In vitro release studies demonstrated that the irradiation of the composite gels with monochromatic green light (10532 nm, 100 mW) increases the release of the encapsulated methylene blue, most likely due to the photothermal effect of Au nanoparticles. This opens the possibility to explore the application of these nanocomposites as carriers in remotely controlled light-triggered drug release.

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Section: In Vitro Mb Releasementioning

confidence: 67%

Effects of Au nanoparticles on thermoresponsive genipin-crosslinked gelatin hydrogels

2013

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“…Existing standards rely upon bulk measurements, such as EDS and XPS, in addition to estimating the surface concentration through functional testing, however using these methods means the precise surface composition would remain unknown. [17][18][19][20][21] The analysis technique detailed herein, provides direct imaging and facilitates quantitative analysis of these materials. Quantification of precise nanoparticle concentrations within the polymer is possible, when the fluorescence images are evaluated against known concentrations of the fluorescent material.…”

Section: Representative Resultsmentioning

confidence: 99%

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Crick

Noimark

Peveler

et al. 2016

JoVE

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The fabrication of polymer-nanoparticle composites is extremely important in the development of many functional materials. Identifying the precise composition of these materials is essential, especially in the design of surface catalysts, where the surface concentration of the active component determines the activity of the material. Antimicrobial materials which utilize nanoparticles are a particular focus of this technology. Recently swell encapsulation has emerged as a technique for inserting antimicrobial nanoparticles into a host polymer matrix. Swell encapsulation provides the advantage of localizing the incorporation to the external surfaces of materials, which act as the active sites of these materials. However, quantification of this nanoparticle uptake is challenging. Previous studies explore the link between antimicrobial activity and surface concentration of the active component, but this is not directly visualized. Here we show a reliable method to monitor the incorporation of nanoparticles into a polymer host matrix via swell encapsulation. We show that the surface concentration of CdSe/ZnS nanoparticles can be accurately visualized through cross-sectional fluorescence imaging. Using this method, we can quantify the uptake of nanoparticles via swell encapsulation and measure the surface concentration of encapsulated particles, which is key in optimizing the activity of functional materials. Video LinkThe video component of this article can be found at

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“…Using different methods of preparation, Au-based nanomaterials such as Au nanospheres, Au nanostars, and Au nanorods can be obtained to inactive bacteria by a photothermic process [73]. Thus, Au-nanoparticles can be conjugated with specific antibody [74], PSs [75], and antibiotics [76], or have intrinsic antibacterial activity [77,78].…”

Section: Photomedicine -Advances In Clinical Practicementioning

confidence: 99%

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

Bloise¹,

Minzioni²,

Imbriani³

et al. 2017

Photomedicine - Advances in Clinical Practice

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Recent developments in light-controlled therapies (e.g., photodynamic and photothermal therapies) provide promising strategies to prevent and suppress bacterial infections, which are a leading cause of morbidity and mortality. Antibacterial photodynamic therapy (aPDT) has drawn increasing attention from the scientific society for its potential to kill multidrug-resistant pathogenic bacteria and for its low tendency to induce drug resistance. In this chapter, we summarize the mechanism of action of aPDT, the photosensitizers, as well the current developments in terms of treating Gram-positive and Gram-negative bacteria. The chapter also describes the recent progress relating to photomedicine for preventing bacterial infections and biofilm formation. We focus on the laser device used in aPDT and on the light-treatment parameters that may have a strong impact on the results of aPDT experiments. In the last part of this chapter, we survey on the various nanoparticles delivering photoactive molecules, and photoactive-nanoparticles that can potentially enhance the antimicrobial action of aPDT.

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The antimicrobial properties of light-activated polymers containing methylene blue and gold nanoparticles

Cited by 238 publications

References 12 publications

Effects of Au nanoparticles on thermoresponsive genipin-crosslinked gelatin hydrogels

Effects of Au nanoparticles on thermoresponsive genipin-crosslinked gelatin hydrogels

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

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