The antimicrobial potential of Streptomyces from insect microbiomes

Chevrette, Marc G.; Carlson, Caitlin M.; Ortega, Humberto E.; Thomas, Chris S.; Ananiev, Gene E.; Barns, Kenneth J.; Book, Adam J.; Cagnazzo, Julian; Carlos, Camila; Flanigan, Will; Grubbs, Kirk J.; Horn, Heidemarie; Hoffmann, F. Michael; Klassen, Jonathan L.; Knack, Jennifer J.; Lewin, Gina R.; McDonald, Bradon R.; Müller, Laura; Melo, Weilan Gomes da Paixão; Pinto‐Tomás, Adrián A.; Schmitz, Amber; Wendt-Pienkowski, Evelyn; Wildman, Scott A.; Zhao, Miao; Zhang, Fan; Bugni, Tim S.; Andes, David R.; Pupo, Mônica Tallarico; Currie, Cameron R.

doi:10.1038/s41467-019-08438-0

Cited by 254 publications

(281 citation statements)

References 59 publications

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“…In co-cultures with pre-cultured S-2, the effect was even more pronounced, resulting in the complete inhibition of fungal growth on plates with bacteria pre-cultured for 7 and 14 days for N. oryzae roseF7 and for all tested fungi, respectively. Recently, there have been a number of reports of new Streptomyces strains isolated from extreme and non-conventional environments that are showing antimicrobial activity [1,13,24,25]. Streptomyces strains have also been previously studied for their potential to protect plants against fungal infections [2,35].…”

Section: Discussionmentioning

confidence: 99%

Genome Mining Revealed a High Biosynthetic Potential for Antifungal Streptomyces sp. S-2 Isolated from Black Soot

Siupka

Piński

Babicka

et al. 2020

IJMS

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The increasing resistance of fungal pathogens has heightened the necessity of searching for new organisms and compounds to combat their spread. Streptomyces are bacteria that are well-known for the production of many antibiotics. To find novel antibiotic agents, researchers have turned to previously neglected and extreme environments. Here, we isolated a new strain, Streptomyces sp. S-2, for the first time, from black soot after hard coal combustion (collected from an in-use household chimney). We examined its antifungal properties against plant pathogens and against fungi that potentially pose threat to human health (Fusarium avenaceum, Aspergillus niger and the environmental isolates Trichoderma citrinoviridae Cin-9, Nigrospora oryzae sp. roseF7, and Curvularia coatesieae sp. junF9). Furthermore, we obtained the genome sequence of S-2 and examined its potential for secondary metabolites production using anti-SMASH software. The S-2 strain shows activity against all of the tested fungi. Genome mining elucidated a vast number of biosynthetic gene clusters (55), which distinguish this strain from closely related strains. The majority of the predicted clusters were assigned to non-ribosomal peptide synthetases or type 1 polyketide synthetases, groups known to produce compounds with antimicrobial activity. A high number of the gene clusters showed no, or low similarity to those in the database, raising the possibility that S-2 could be a producer of novel antibiotics. Future studies on Streptomyces sp. S-2 will elucidate its full biotechnological potential.

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Section: Discussionmentioning

confidence: 99%

Genome Mining Revealed a High Biosynthetic Potential for Antifungal Streptomyces sp. S-2 Isolated from Black Soot

Siupka

Piński

Babicka

et al. 2020

IJMS

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“…The pieces were homogenized in phosphate saline solution with glass beads (4mm-Marienfield) using a vortex. 100μL of 10-fold serial dilutions in phosphate saline solution were plated on four culture media: a) Actinomycete Isolation Agar (AIA, Difco, BD), b) International Streptomyces Project medium-2 (ISP2), both supplemented with nalidixic acid (150mg/L) and nystatin (50mg/L) (67,89), c) Gause Synthetic medium, and d) Gause Oligotrophic supplemented with potassium dichromate (80mg/L) (90).…”

Section: Bacterial Isolation and Antimicrobial Screenmentioning

confidence: 99%

The microbiomes of seven lichen genera reveal host specificity, a reduced core community and potential as source of antimicrobials

Sierra

Danko

Sandoval

et al. 2019

Preprint

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The High Andean Paramo ecosystem is a unique neotropical mountain biome considered a diversity and evolutionary hotspot. Lichens, which are complex symbiotic structures that contain diverse commensal microbial communities, are prevalent in Paramos. There they play vital roles in soil formation and mineral fixation. In this study we analyzed the microbiomes of seven lichen genera in two Colombian Paramos using 16S rRNA gene analyses and provide the first description of the bacterial communities associated with Cora and Hypotrachyna lichens. Paramo lichen microbiomes were diverse, and in some cases were distinguished based on the identity of the lichen host. The majority of the lichen-associated microorganisms were not present in all lichens sampled and could be considered transient or specialists.We also uncovered sixteen shared taxa that suggest a core lichen microbiome among this diverse group of lichens, broadening our concept of these symbiotic structures. Additionally, we identified strains producing compounds active against clinically relevant pathogens. These results indicate that lichen microbiomes from the Paramo ecosystem are diverse and host-specific but share a taxonomic core and can be a source of new bacterial taxa and antimicrobials.Lichens represent some of the oldest and most diverse symbioses on Earth (16). Lichens consist of a photobiont (cyanobacterium/alga) and a mycobiont (fungus), which together form a unique structure called the thallus (17). Lichens play a vital role in ecosystems as they are essential in soil formation, naked soil colonization, and nutrient uptake and release for plants (18,19). Lichens can colonize a wide range of substrates, from natural surfaces to man-made materials such as plastic, rubber, metals and glass (20). They can also tolerate extreme environmental conditions and offer a niche for diverse microorganisms (21,22). The diversity of these lichen-associated microbial communities is not yet well characterized, and has only recently been investigated using high-throughput techniques (23, 24). These studies indicate substantial microbial and functional diversity (24-26) that has been suggested to help protect the thalli against pathogens through the production of antimicrobials (27,28). The process of community establishment within lichens is poorly understood and has been proposed to be driven either by the photobiont (29) or by geography and habitat (30).Recent studies have described the microbial communities associated with lichens using cultureindependent strategies (24, 25). However, comparisons between studies are hindered by differences in sampling methods, data analyses, and poor or complete lack of lichen description. Given the complexity of the lichen symbiotic structures, with recent evidence indicating that some lichens may be composed of multiple bacteria and more than one fungus (26), it is important to study lichen microbiomes in order to understand their ecological role in the symbiosis.A large and unexplored diversity of lichens is located in the Par...

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“…Earlier in 2019, a team led by University of Wisconsin-Madison professor Cameron Currie made headlines when it published the results of an exhaustive search of microbes from more than 1400 insects. 6 The study is the largest and most thorough to assess insect-associated microbes for antibiotic activity to date.…”

Section: Insect Bonanzamentioning

confidence: 99%

Drugs from bugs

2019

Chemistry & Industry

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The antimicrobial potential of Streptomyces from insect microbiomes

Cited by 254 publications

References 59 publications

Genome Mining Revealed a High Biosynthetic Potential for Antifungal Streptomyces sp. S-2 Isolated from Black Soot

Genome Mining Revealed a High Biosynthetic Potential for Antifungal Streptomyces sp. S-2 Isolated from Black Soot

The microbiomes of seven lichen genera reveal host specificity, a reduced core community and potential as source of antimicrobials

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