The antimicrobial peptide cecropin A induces caspase-independent cell death in human promyelocytic leukemia cells

Ceron, J. M.; Contreras-Moreno, J.; Puertollano, Elena; Cienfuegos, Gerardo Álvarez de; Puertollano, Marı́a A.; Pablo, Manuel A. de

doi:10.1016/j.peptides.2010.05.008

Cited by 77 publications

(49 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A biochemical hallmark of apoptosis was the chromatin cleavage including oligonucleosomes, which were depicted as DNA ladders in the electrophoresed gel 27 . Similar results were observed in cecropin treated human promyelocytic leukemia HL-60 cell line 28 . Magainin I or BMAP-28 (bovine cathelicidinderived AMP), cationic peptides which also induced DNA fragmentation 17,29 .…”

Section: Fig 1: Determination Of Cytotoxicity (A) Dose-dependent Cysupporting

confidence: 85%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

Antimicrobial peptides (AMP) showed a broad spectrum of cytotoxic activity against cancer cells, which could provide a new class of anticancer drugs. In this study, we have evaluated the cytotoxic and apoptotic activity of magainin II, an AMP compared with 5-Fluorouracil (5-FU) in colo320 DM cancer cells, and magainin II cytotoxicity was assessed on vero cell line by MTT. The cells cultured in the presence or absence of magainin II and 5-FU. The magainin II inhibited cell viability in dose dependent manner was quantified by MTT assay. The IC 50 of magainin II was found to be 89.3nM and at this concentration the cytotoxicity was not observed in vero cell line. The cytotoxicity of the magainin II was further confirmed by lactate dehydrogenase (LDH) assay and neutral red uptake assay (NRU). Apoptosis induction was verified by DNA fragmentation analysis. The morphological changes in colo 320 DM cells was measured by DAPI and acridine orange/ethidium bromide staining, showed nuclear condensation, and this substantiates the apoptotic action of magainin II. The cell cycle analysis by FACS revealed that there was a accumulation of cells at G0/G1 phase in magainin II treated cells. Overall, these results revealed that magainin II has selective cytotoxicity in cancer cells and has the potential to induce apoptosis. Magainin II may offer a novel and effective therapeutic candidate in the treatment of colon cancer with potentially low cytotoxic effects on normal cells. These findings highlight the importance of magainin II as a drug capable of exerting an in vitro antitumoral activity by triggering apoptosis.

show abstract

Section: Fig 1: Determination Of Cytotoxicity (A) Dose-dependent Cysupporting

confidence: 85%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

show abstract

“…Thus, apoptosis and lytic characters co-exist and are the potential mechanisms of pardaxin-induced cell death (Wu et al, 2012). Likewise, Ceron et al (2010) found that antimicrobial peptide Cecropin A has antitumor effect. The results displayed that the human promyelocytic leukemia cell HL-60 treated with cecropin A, the cells presented classical characters of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Fused Polypeptide with DEF Induces Apoptosis of Lung Adenocarcinoma Cells

Liang

Zhang²,

Zhou³

et al. 2013

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

To analyze the effects of a new unknown peptide DEF on the growth of tumor cells, a fused polypeptide TAT-DV1-DEF was designed and synthesized. The lung adenocarcinoma cell line GLC-82 treated with TAT-DV1-DEF was analyzed with a cell counting kit 8, and the location of polypeptides in cells was observed under laser confocal microscopy. The efficiency of polypeptide transfection and changes in nuclear morphology were analyzed by flow cytometry and fluorescence microscopy, respectively. Finally, the mechanism of tumor cell growth inhibition was evaluated by Western blotting. We found that TAT-DV1-DEF could significantly inhibit the growth of the lung adenocarcinoma cell line GLC-82, but not the normal human embryonic kidney cell line HEK-293. Polypeptides were found to be mostly localized in the cytoplasm and some mitochondria. The efficiency of polypeptide transfection in the two cell types was approximately 99%. Apoptotic nuclei were observed under fluorescence microscopy upon treatment with polypeptides and DAPI staining. Western blot analyses indicated that the polypeptide inhibition of tumor cell growth was apoptosis dependent. In the present study, we demonstrated that fused polypeptides could induce apoptosis of the lung adenocarcinoma cell line GLC-82, indicating that the new unknown peptide DEF has antitumor effects.

show abstract

“…Cerón et al (26) reported that cecropin A was able to induce apoptosis in HL-60 cells through the production of ROS but independent of caspase activation. Huang et al (42) showed that pardxin, an antimicrobial peptide was first isolated from secretions of the Red Sea Moses sole, exerted antitumor effects on human fibrosarcoma HT-1080 cells by increasing intracellular ROS and activating caspase-3/7 activities.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that cancer cell apoptosis induced by cecropins may be due to mitochondrial dysfunction and overproduction of ROS (26). Intracellular ROS production and loss of Δψ m cause mitochondrial membrane depolarization and trigger a cascade of mitochondrion-dependent apoptotic signaling (27).…”

Section: Cecropinxj Promotes Ros Production and Decreases Mitochondrimentioning

confidence: 99%

CecropinXJ inhibits the proliferation of human gastric cancer BGC823 cells and induces cell death in vitro and in vivo

et al. 2015

International Journal of Oncology

View full text Add to dashboard Cite

Abstract. We have shown that an antimicrobial peptide (AMP) cecropinXJ isolated from the larvae of Bombyx mori selectively inhibits the proliferation of cancer cells. However, the mechanism remains to be determined. In the present study, we examined the antitumor activity of cecropinXJ against human gastric cancer BGC823 cells and explored the mechanism. The results showed that cecropinXJ inhibited the growth of gastric cancer BGC823 cells in vitro and in vivo. MTT and colony formation assays indicated that cecropinXJ suppressed cell proliferation and reduced colony formation of BGC823 cells in a dose-and time-dependent manner, but without inhibitory effect on normal gastric epithelia GES-1 cells. S-phase arrest in BGC823 cells was observed after treatment with cecropinXJ. Annexin V/PI staining suggested that cecropinXJ induced both early and late phases of apoptosis through activation of mitochondrial-mediated caspase pathway, upregulation of Bax expression and downregulation of Bcl-2 expression. Additionally, cecropinXJ treatment increased reactive oxygen species (ROS) production, disrupted the mitochondrial membrane potential (Δψ m ) and led to release of cytochrome c. Importantly, in vivo study showed that cecropinXJ significantly prevented the growth of xenograft tumor in the BGC823-bearing mice, possibly mediated by the induction of apoptosis and inhibition of angiogenesis. These results suggest that cecropinXJ may be a promising therapeutic candidate for the treatment of gastric cancer. IntroductionGastric cancer is one of the most common malignant tumors, >70% of new cases and deaths occur in developing countries each year (1). In China, the incidence and mortality rate of gastric cancer are increasing. Although there are distinct discrepancies in diagnosis, prognosis, and treatment efficacy for gastric cancer patients, the 5-year survival rate of gastric cancer is generally <20% (2). At present, the management of gastric cancer includes surgery, radiotherapy, conventional chemotherapy, molecular targeted therapy and biological therapy, but these methods have not reached expected clinical efficacy, which mainly focus on mass cell killing without high specificity and often causes severe side-effects and toxicities. Moreover, recurrences and metastasis frequently occur in the majority of patients (2). Hence, it is urgent to explore safe and effective therapeutic agents for gastric cancer to improve the survival rate and quality of life.Antimicrobial peptides (AMPs) are evolutionarilyconserved components of the innate immune system (3), which exert their activities against a broad range of microbial pathogens. Compared to conventional treatments, AMPs have many advantages, such as specific cytotoxicity for cancer cells, ability to bypass the multidrug-resistance mechanism, and additive effects in combination therapy (4), suggesting that AMPs has potential as a novel antitumor agent for the treatment of cancer.AMPs can directly kill cancer cells by a cell membranelytic effect (5), or trigger apoptosis in can...

show abstract

The antimicrobial peptide cecropin A induces caspase-independent cell death in human promyelocytic leukemia cells

Cited by 77 publications

References 47 publications

Untitled

Untitled

Fused Polypeptide with DEF Induces Apoptosis of Lung Adenocarcinoma Cells

CecropinXJ inhibits the proliferation of human gastric cancer BGC823 cells and induces cell death in vitro and in vivo

Contact Info

Product

Resources

About