The Antimicrobial Pattern of E. coli Isolated from Various Animal Sources at El Behera Governorate

Abstract

The emergence of multidrug resistant E. coli isolated from animal and animal products become the main wary of food safety and public health authorities worldwide. The present study was undertaken to recognize the most important serovars and the antimicrobial sensitivity patterns of E. coli isolated from animals and animal products. Results showed that out of the 300 examined samples (75 cows mastitic milk, 77 buffalo's mastitic milk, 40 cattle meat, 43 buffaloes meat and 65 chicken meat), 60 isolates (20 %) were confirmed as E. coli based on cultural characteristic and biochemical identification. These isolates were further identified serologically, the obtained isolates were categorized as O26:H11 (4/22, 18%), O91:H21 (3/22, 13.6%), O111:H2 (2/22, 9%), O146:H21 (2/22, 9%), O1:H7 (1/22, 4.5%), O55:H7 (1/22, 4.5%), O119:H6 (1/22, 4.5%), O121:H7 (1/22, 4.5%), O78 (2/22, 9%), O125: H21 (2/22, 9%), O128 : H2 (1/22, 4.5%), O159 (1/22, 4.5%), and O44:H18 (1/22, 4.5%). The isolates were further tested for antimicrobial resistance against 14 commercial antibiotics. The obtained results showed that the isolates were resistant to Erythromycin (22/22, 100٪), Streptomycin (21/22, 95.5٪), Nalidixic acid (17/22, 80٪), Clindamycin (14/22, 63.6٪) and Sulphamethozole (11/22, 50٪). Lower resistance rate was observed in case of Cephalothin (9/22, 40.9٪), Tetracycline (7/22, 31.8٪), Ampicillin (6/22, 27.3٪), Colistin (5/22, 22.7٪), Ciprofloxacin (5/22, 22.7٪), Amikacin (4/22, 18.2٪), Levofloxacin (2/22, 9.1٪), Gentamycin (2/22, 9.1٪) and Meropenem was (1/22, 4.5٪). In conclusion, there a wide variation in E. coli serogroups isolated from different animals and animal products. These serogroups exhibited a high multidrug resistance index against the commonly used antibiotics. Therefore, regular monitoring of the potential sources of human infection with E. coli will help establish accurate preventive and curative measures.

Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant Escherichia coli O157:H7 and O55:H7 in Camel Meat

“…Our results are in line with those reported in a previous study conducted in Italy by Grispoldi et al [ 52 ], who found that 89.7% of E. coli from bovine lymph nodes were resistant to cephalothin, which is recognized as an effective antimicrobial against Gram-negative as well as Gram-positive bacteria. Furthermore, a relatively lower resistance rate of 57% was observed in E. coli serovars isolated from bovine origin meat samples (four cows and three buffaloes) in Egypt against cephalothin [ 53 ]. Interestingly, despite the two third-generation cephalosporins, namely ceftriaxone and cefotaxime, being similar in their antibacterial spectrum, indications, and route of administration, the isolates examined showed a resistance rate of 29.4% against cefotaxime and a resistance rate of only 3.97% against ceftriaxone ( Table 2 ), which may be attributed to the difference in the administration dose and elimination half-life between the two antibiotics (ceftriaxone: 2 g once a day with half-life elimination of 8.8 h versus cefotaxime: 2 g every 4 h, 1.2 h) [ 54 , 55 ].…”