The Antimicrobial Effectiveness of Chitosan and Polysaccharide of Durian Peel Extract against Mycobacterium tuberculosis Isolates

Abstract

BACKGROUND: Tuberculosis (TB) disease is an infection caused by Mycobacterium tuberculosis and is transmitted through sputum droplets of sufferers or suspect TB in the air. Chitosan as an antimicrobial agent can be used in the biomedical field because it has a number of hydroxyl groups (OH) and amine groups (NH2). The chemical substance of durian peel extract (Durio zibethinus L.) contains pectin which is multifunctional and can be used in the pharmaceutical field. Chemically, pectin is a polysaccharide polymer of D-galacturonic acid linked by -1,4 glycosidic bonds which can dissolve in water to form colloidal solutions or gels. AIM: This study was to determine the antimicrobial effectiveness of chitosan and polysaccharides from durian peel extract (D. zibethinus L.) against M. tuberculosis isolates in vitro. METHODS: The research method is based on an experimental study in vitro. M. tuberculosis isolates in this research from sputum samples of patients suspected of TB in Surakarta Regional General Hospital. The stages of the research were performed that preparation chitosan gel (CG), bioformulation of CG, and polysaccharide gel (PG) durian peel extract are 5%, 10%, 15%, 20%, and 25%, and drug susceptibility testing against M. tuberculosis isolates. RESULTS: CG 10% was effective as an antimicrobial against M. tuberculosis isolates but PG durian peel extract (5%; 10%; 15%; 20%; and 25%) was not effective as an antimicrobial against M. tuberculosis isolates. The types of anti-tuberculosis drug (ATD) that was effective against M. tuberculosis isolates were ethambutol 80% and streptomycin 40%, while isoniazid and rifampicin were not effective as ATD against M. tuberculosis isolates. CONCLUSION: CG 10% was effective as an antimicrobial against M. tuberculosis isolates, while PG 25% durian peel extract was not effective as an antimicrobial against M. tuberculosis isolates. CG has the potential as an ATD based on natural bioactive ingredients for TB therapy.

The Impact of the COVID-19 Pandemic on the Resistance Level of Mycobacterium Tuberculosis in Suspected Tuberculosis Patients at the Surakarta Central General Hospital

“…The results showed that the combination of snail seromucoid and chitosan has potential as a bioimmunostimulator and bioimmunodulator in TB therapy (Sutanto et al, 2020;Sutanto et al,2021a;Sutanto et al, 2021b). Durian peel extract polysaccharides and chitosan have the potential to an antimicrobial against MTb isolates (Sutanto et al, 2022).…”