The Antimicrobial Effect of Lactobacillus Casei Culture Supernatant Against Multiple Drug Resistant Clinical Isolates of Shigella Sonnei and Shigella Flexneri in Vitro

Mirnejad, Reza; Vahdati, Ali R.; Rashidiani, Jamal; Erfani, Mohammad; Piranfar, Vahhab

doi:10.5812/ircmj.7454

Cited by 62 publications

(39 citation statements)

References 18 publications

(12 reference statements)

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“…The protective effects of Lactobacilli, probiotic bacteria, are greater against invasive bacteria such as S. sonnei, when compared to non-invasive bacteria such as Vibrio cholerae (34). However, the mechanism of the inhibitory effect of lactic acid bacteria on Shigella has been mostly investigated in vitro (35,36), and it is not fully understood how these inhibitory effects are exerted on pathogenic bacteria in the complex intestinal microecological environment. These inhibitory effects may be associated with decreases in host gene expression, microRNA regulation and a substantial reshaping of the Listeria-monocytogenes transcriptome (37).…”

Section: Discussionmentioning

confidence: 99%

Dynamic comparison of gut microbiota of mice infected with Shigella�flexneri via two different infective routes

Yang

Chen²,

Xia³

et al. 2020

Exp Ther Med

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Shigella is one of the main pathogens causing diarrheal disease, and is associated with high morbidity and mortality in developing countries. Previous clinical data and animal studies have shown that the outcomes of oral and peritoneal infections of Shigella differ, and that the latter is more serious. Furthermore, a variety of pathogenic bacteria are known to cause changes in intestinal flora after infection, and the influence of Shigella infection on intestinal flora remains poorly understood. In the present study, the 16S rRNA high-throughput sequencing method was used to compare the changes in gut microbiota profiles in feces of mice infected with Shigella via two routes. In addition, the present study investigated the association between the differences in infection performance and bacterial communities. The present results suggested that the intraperitoneal route induced a distinct decrease in α-diversity in the fecal microbiota when compared to the control at a later time, while the effect of the oral route on α-diversity was not obvious. Oral infection of Shigella had a rapid and significant effect on gut microbiota, mainly causing a decreased abundance of Lactobacillus and an increased abundance of Prevotella and Escherichia/Shigella in the early stage of infection. By contrast, the effect of intraperitoneal infection on the gut microbiota was relatively slow and small. The principal coordinate analysis results suggested that the dynamic profile of gut microbiota between the two infective routes was consistent with the infection process. Probiotics, such as Lactobacillus reuteri and Faecalitalea exhibited significantly reduced abundance after Shigella infection. Collectively, the present results suggested that gut microbiota may play a pivotal role in the pathogenesis of Shigella infection. Future studies should investigate the effect of Shigella infection on the interaction between pathogenic bacteria and intestinal flora. The present results suggested that the use of probiotics may facilitate the prevention and treatment of shigellosis.

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Section: Discussionmentioning

confidence: 99%

Dynamic comparison of gut microbiota of mice infected with Shigella�flexneri via two different infective routes

Yang

Chen²,

Xia³

et al. 2020

Exp Ther Med

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“…The emergence of multiple drug resistance to cost-effective antimicrobials against Shigella is a matter of concern in developing countries, and resistance pattern of this bacterium is the cause of numerous clinical problems worldwide [83]. Due to increased prevalence of its antibiotic resistance, the need for alternative treatment has therefore been deemed necessary.…”

Section: The Use Of Conventional Probiotics For Control Of Selected Fmentioning

confidence: 99%

Probiotic engineering: towards development of robust probiotic strains with enhanced functional properties and for targeted control of enteric pathogens

2017

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There is a growing concern about the increase in human morbidity and mortality caused by foodborne pathogens. Antibiotics were and still are used as the first line of defense against these pathogens, but an increase in the development of bacterial antibiotic resistance has led to a need for alternative effective interventions. Probiotics are used as dietary supplements to promote gut health and for prevention or alleviation of enteric infections. They are currently used as generics, thus making them non-specific for different pathogens. A good understanding of the infection cycle of the foodborne pathogens as well as the virulence factors involved in causing an infection can offer an alternative treatment with specificity. This specificity is attained through the bioengineering of probiotics, a process by which the specific gene of a pathogen is incorporated into the probiotic. Such a process will subsequently result in the inhibition of the pathogen and hence its infection. Recombinant probiotics offer an alternative novel therapeutic approach in the treatment of foodborne infections. This review article focuses on various strategies of bioengineered probiotics, their successes, failures and potential future prospects for their applications.

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“…Among the potential varieties of alternative anti-bacterial preparations with notable anti-microbial properties against polyresistant pathogens, the advantage belongs to probiotic live bacteria and their exometabolites (Mirnejad, 2013). Optimistic prognoses which were made earlier, and have been confirmed over recent years emerged due to a new approach to using products of vital activity of probiotic microorganisms for treatment and prevention of diseases of different genesis.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of anti-microbial activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii against antibiotic-resistant gram-negative bacteria

Isayenko

Knysh

Kotsar

et al. 2019

Regul. Mech. Biosyst.

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The article presents the results of the first study on the influence of biologically active substances Lactobacillus rhamnosus GG ATCC 53103 and Saccharomyces boulardii, obtained according to the author`s method, on growth of gram-negative bacteria with broad medical resistance: Pseudomonas aeruginosa PR, Klebsiella pneumoniae PR, Lelliottia amnigena (Enterobacter amnigenus) PR using the spectrophotometric method. Disintegrates of L. rhamnosus GG and S. boulardii were obtained using low-frequency ultrasound processing of suspension of probiotic strains, and metabolites – through cultivation of lactobacteria and saccharomycetes in disintegrates of probiotic microorganisms. To samples of test-cultures with studied filtrates of disintegrates or metabolites we added growth medium and cultivated them (period of monitoring was 5- and 24-hours). Results of the studies were expressed as the percentage of inhibition of increment in polyresistant gram-negative bacteria under the impact of biologically active substances of probiotic microorganisms. Five-hour incubation of test-strains with the studied samples of lactobacteria led to inhibition of their growth properties by 85.6–96.7%. Growth of bacteria under the impact of substances of saccharomycetes was inhibted by 45.1–92.5%. Twenty-four hour exposure of the test-cultures in filtrates of L. rhamnosus GG and S. boulardii caused 100% inhibition of P. aeruginosa and L. amnigena polyresistant strains. Temporal interval of cultivation directly proportionally affected the extent of inhibition of growth of microorganisms: we determined direct correlation dependence within 0.789–0.991. Maximum inhibition of increment of the studied pathogens was observed under the influence of metabolites of lactobacteria, obtained by cultivating primary producers in their disintegrate. We determined a high level of anti-microbial activity of metabolites from L. rhamnosus GG and S. boulardii obtained by cultivation of probiotics in disintegrates against bacteria resistant to a broad range of preparations, which allows us to consider these substances as promising for development of anti-microbial preparations of a new generation against etiologically significant antibiotic-resistant gram-negative microorganisms.

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The Antimicrobial Effect of Lactobacillus Casei Culture Supernatant Against Multiple Drug Resistant Clinical Isolates of Shigella Sonnei and Shigella Flexneri in Vitro

Cited by 62 publications

References 18 publications

Dynamic comparison of gut microbiota of mice infected with Shigella�flexneri via two different infective routes

Dynamic comparison of gut microbiota of mice infected with Shigella�flexneri via two different infective routes

Probiotic engineering: towards development of robust probiotic strains with enhanced functional properties and for targeted control of enteric pathogens

Evaluation of anti-microbial activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii against antibiotic-resistant gram-negative bacteria

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