The antimicrobial effect of Pulsatilla Koreana extracts to oral micro-organism

Chung, Sung-Wha; Chung, Chin-Hyung; Lim, Sung-Bin; Kim, Jung-Keun; So, Eun-Hee

doi:10.5051/jkape.2000.30.3.661

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As evidenced in studies on the pharmacological use of components isolated from the extract, its protoanemonins show antifungal properties and antibiotic activity. Moreover, the biologically active compounds isolated from the root extract of P. koreana have been found to show anticancer [73], anti-inflammatory [74], antiparasitic [75], and antibacterial [76] activities.…”

Section: Plasmodium Falciparum (D6 % Inhibition)mentioning

confidence: 99%

Phytochemical screening of Pulsatilla species and investigation of their biological activities

et al. 2019

View full text Add to dashboard Cite

The present study aimed to identify biologically active secondary metabolites from the rare plant species, Pulsatilla patens subsp. patens and the cultivated P. vulgaris subsp. vulgaris. Chromatographic fractionation of the ethanolic extract of the roots of P. patens subsp. patens resulted in the isolation of two oleanane-type glycosides identified as hederagenin 3-O-β-d-glucopyranoside (2.7 mg) and hederagenin 3-O-β-d-galactopyranosyl-(1→2)-β-d-glucopyranoside (3.3 mg, patensin). HPLC analysis of the methanolic extract of the crude root of P. patens subsp. patens and P. vulgaris subsp. vulgaris revealed the presence of Pulsatilla saponin D (hederagenin 3-O-α-l-rhamnopyranosyl(1→2)-[β-d-glucopyranosyl(1→4)]-α-l-arabinopyranoside). Chromatographic analysis using GC-MS of the silylated methanolic extracts from the leaves and roots of these species identified the presence of carboxylic acids, such as benzoic, caffeic, malic, and succinic acids. The extracts from Pulsatilla species were tested for their antifungal, antimicrobial, and antimalarial activities, and cytotoxicity to mammalian cell lines. Both P. patens subsp. patens and P. vulgaris subsp. vulgaris were active against the fungus Candida glabrata with the half-maximal inhibitory concentration (IC50) values of 9.37 µg/mL and 11 µg/mL, respectively. The IC50 values for cytotoxicity evaluation were in the range of 32–38 μg/mL for P. patens subsp. patens and 35–57 μg/mL for P. vulgaris subsp. vulgaris for each cell line, indicating general cytotoxic activity throughout the panel of evaluated cancer and noncancer cells.

show abstract

Section: Plasmodium Falciparum (D6 % Inhibition)mentioning

confidence: 99%

Phytochemical screening of Pulsatilla species and investigation of their biological activities

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Similarly, the saponin D from P. koreana Nakai inhibited the growth of cancer cells [ 22 , 23 ] and is used in the treatment of Alzheimer’s disease [ 24 ]. From the roots of P. koreana, 11 saponins were isolated, including five lupane-type, and one oleanane [ 25 ], which showed anticancer [ 26 ], antibacterial [ 27 ], antiparasitic [ 28 ], anti-inflammatory [ 29 ], antifungal and antibiotic activities [ 25 ]. The saponins produced by P. chinensis [ 14 , 15 ], P. koreana [ 23 , 25 ], P. pratensis (L.) Mill.…”

Section: Introductionmentioning

confidence: 99%

Pulsatilla vulgaris Inhibits Cancer Proliferation in Signaling Pathways of 12 Reporter Genes

Łaska

Sieniawska

Maciejewska-Turska

et al. 2023

IJMS

View full text Add to dashboard Cite

This study aimed to examine if methanolic extracts of Pulsatilla vulgaris Mill. can inhibit HeLa cell proliferation through the modulation of cancer-related signaling pathways. The cytotoxicity and chemical composition of P. vulgaris leaves and root extracts were also determined. Research showed that root extract of P. vulgaris inhibited 12 signaling pathways in a cervical cancer cell line and the most potent activation inhibition was observed for MYC, Notch, Wnt, E2F, Ets, Stat3, Smad, Hdghog, AP-1, and NF-κB, at a concentration of 40 µg/mL. The methanolic extracts of P. vulgaris enhanced apoptotic death and deregulated cellular proliferation, differentiation, and progression toward the neoplastic phenotype by altering key signaling molecules required for cell cycle progression. This is the first study to report the influence of P. vulgaris on cancer signaling pathways. Additionally, our detailed phytochemical analysis of the methanolic extracts of P. vulgaris gives a conclusion that compounds, which strongly suppressed the growth and proliferation of HeLa cancer cells were mainly triterpenoid saponins accompanied by phenolic acids.

show abstract

Antibacterial effect of bamboo charcoal on Streptococcus mutans

Choi¹,

Ahn²

2014

J Korean Soc Dent Hyg

View full text Add to dashboard Cite

Objectives : The purpose of the present study was to investigate the effect of bamboo charcoal on Streptococcus mutans which is one of the most important causative agents of dental caries.Methods : S. mutans was incubated with or without bamboo charcoal and then changes were observed in its cell viability and antibacterial effect. Oral epithelial cells viabillity(human gingival fibroblast, HGF) was performed using MTT assay. Antibacterial effect was analyzed using a dilution plating method and agar diffusion method. Results : Oral epithelial cells, human gingival fibroblast (HGF) showed a tendency to increase in bamboo charcoal treatment solution concentrations(0.5 , 1, 2 , 3 , 5, 10%). The bamboo charcoal had an antibacterial effect on S. mutans. Antibacterial effect of bamboo charcoal for the bacterium was 58%. Charcoal concentration of 2% and 5% in the inhibition zone showed a minimal growth, but the concentration of 10% bamboo charcoal in inhibition zone revealed a conspicuous antibacterial activity.Conclusions : Overall results suggested that the bamboo charcoal proved to be bactericidal effect on S. mutans.

show abstract

The antimicrobial effect of Pulsatilla Koreana extracts to oral micro-organism

Cited by 3 publications

References 0 publications

Phytochemical screening of Pulsatilla species and investigation of their biological activities

Phytochemical screening of Pulsatilla species and investigation of their biological activities

Pulsatilla vulgaris Inhibits Cancer Proliferation in Signaling Pathways of 12 Reporter Genes

Antibacterial effect of bamboo charcoal on Streptococcus mutans

Contact Info

Product

Resources

About