The Antimicrobial and Phytochemical Analysis of the Leaves of Aspilia africana on Clinical Isolates

Ezeigbo, O. R.; Awomukwu, Daniel Azubuike; Ezeigbo, I

doi:10.9734/ejmp/2016/26481

Cited by 6 publications

(3 citation statements)

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“…Considering the etiologic roles of P. aeruginosa in the wound and biofilmassociated infections, which risk especially increases with the prolonged use of in dwelling prosthetic and catheter devices (Dosler and Kraaslan 2014;Sharma et al, 2014;Obioma et al, 2017), the result suggests prospect of A. africana-based formulations for wound and resistance infection treatment. A similar observation of the high activity of the plant against P. aeruginosa was also, made in another study (Ezeigbo et al, 2016). Unlike the methanolic extract with good antibacterial activity, the effect of the aqueous extract of A.…”

Section: Discussionsupporting

confidence: 80%

Investigation of the antibacterial activities of methanolic and water extracts of Gongronema latifolium, Psidium guajava and Aspilia africana

Chidebelu¹,

Chigor²,

Ojinna³

et al. 2021

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The epidemiological consequences of co-infections by enteric, wound, opportunistic, and drug-resistant pathogens, underscore the need for alternative and multi-target therapeutic approaches. Phytochemical properties of some plants are widely known but their potential utility as cheaper multi-target therapeutic options, have rarely been investigated. In the current work, Gongronema latifolium, Psidium guajava, and Aspilia africana extracts were studied for their potential utility as multi-target therapeutic alternatives. The plants were screened for phytochemical constituents. Based on the CLSI disc diffusion assay, the sensitivities of Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, and Pseudomonas aeruginosa to the plants’ extracts were tested. The minimum inhibitory concentration of the extracts against susceptible bacteria was determined by CLSI broth microdilution protocols. The major families of plant bioactive compounds were detected, with alkaloids, terpenoids, and flavonoids common to the three plants investigated. The plant extracts showed variable activity against the test bacteria (activity index, AI range = 0.43 – 2.59), while the highest activity based on the MIC was recorded for P. guajava (MIC range = 25- 100mg/ml). These results provide the basis of the plants’ therapeutic uses in folk medicine. Their activity index suggests the need for further investigation of their phytochemical components for potential medicinal application. The P. guajava methanolic extract with consistent activity across the test bacteria suggests its potentials in the formulation of multi-target antibiotic therapies

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Section: Discussionsupporting

confidence: 80%

Investigation of the antibacterial activities of methanolic and water extracts of Gongronema latifolium, Psidium guajava and Aspilia africana

Chidebelu¹,

Chigor²,

Ojinna³

et al. 2021

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show abstract

“…Blood samples were collected intraperitoneally from all the rats, the serum obtained using a centrifuge for 10 minutes at a speed of 3000 rpm and stored in the refrigerator at a temperature of -20 o C until they are needed for lipid profiling. The level of hyperlipidemia attained was measured using Randox commercial assay kits [14]. Blood lipid levels including TC, TG, HDL and LDL was measured accordingly.…”

Section: Induction Of Hyperlipidemiamentioning

confidence: 99%

Comparative Hypolipidemic Evaluation of Aframomum Melegueta Seeds and Moringa Oleifera Leaves

Nwankwo

Onyegbule

Abba

et al. 2021

JPRI

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Aims: The study was carried out to compare the lipid lowering effects of both crude drugs as well as deducing the extracts with the best lipid lowering property; and the fractions. Study Design: The research was conducted with an experimental design solely based on laboratory trials which involved the use of ninety-six (96) male albino wistar rats to compare the hypolipidemic effects of both crude drugs and respective fractions. Place and Duration of Study: Department of Pharmacognosy Laboratory, Faculty of Pharmacy, Delta State University, Abraka, Nigeria and Department of Pharmacology and Toxicology Laboratory, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Agulu, Anambra State, Nigeria. The research was carried out from March to August, 2021. Methodology: Ethanol extracts of both plants were prepared using soxhlet extraction. Each extract was then subjected to VLC fractionation using four solvents: n-hexane, chloroform, ethyl acetate and methanol. The fractions were bulked together after conducting thin layer chromatographic procedures and each extract was bulked into four fractions. The acute toxicity studies (LD50) of both extracts were determined in the rats using Lorke’s method. The crude extracts were screened for the presence and quantity of phytoconstituents using standard methods. The antilipidemic study was carried out using sixty-eight (68) rats randomized into seventeen (17) groups of four (4) animals each. Lipid profile was determined using spectrophotometer. Liver function tests and histology was also carried out using standard procedures. Results: Administration of various treatments (both crude extracts and fractions) evoked a significant (p<0.05) reduction of TC, TG, and LDL-C as well as significant (p<0.05) elevation of HDL-C when compared with the negative control. With a percentage serum lipid reduction of 45.11%TC, 48.23%TG, 63.39% LDL-C and 174.69% elevation of HDL-C, the group treated with the combination of 500 mg/kg Aframomum melegueta and 500 mg/kg Moringa oleifera produced the best hypolipidemic effect. This is closely followed by fraction MO4. Comparatively, Moringa oleifera extracts exerts a better antilipidemic effect than Aframomum melegueta seed extract. The liver function test showed that both plants has no toxic effect on the liver cells at doses of 250 mg/kg and 500 mg/kg, hence confirming the hepatoprotective effect of both crude drugs at the doses administered. Conclusion: In conclusion, results from this study suggests that ethanol extract of Moringa oleifera leaves is more effective than ethanol extract of Aframomum melegueta seeds as a hypolipidemic agent, however, combination of both crude drugs as a lipid lowering agent has proved to be more effective and reliable when compared to each crude drug administered independently.

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“…Indeed, anti-inflammatory activity is essential for wound healing, since a long duration of the inflammatory phase causes delay in the wound healing process [79]. Additionally, the strong antimicrobial activities of A. africana play a vital role in the ability of this plant to heal wound sepsis [80–84]. In fact, a study by Anibijuwon et al [85] showed that A. africana has strong antimicrobial activities.…”

Section: Effects Of Leaf Extracts Of Aspilia Africana On Wound Heamentioning

confidence: 99%

The Wound Healing Potential of Aspilia africana (Pers.) C. D. Adams (Asteraceae)

Komakech

Matsabisa

Kang

2019

Evidence-Based Complementary and Alternative Medicine

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Wounds remain one of the major causes of death worldwide. Over the years medicinal plants and natural compounds have played an integral role in wound treatment. Aspilia africana (Pers.) C. D. Adams which is classified among substances with low toxicity has been used for generations in African traditional medicine to treat wounds, including stopping bleeding even from severed arteries. This review examined the potential of the extracts and phytochemicals from A. africana, a common herbaceous flowering plant which is native to Africa in wound healing. In vitro and in vivo studies have provided strong pharmacological evidences for wound healing effects of A. africana-derived extracts and phytochemicals. Singly or in synergy, the different bioactive phytochemicals including alkaloids, saponins, tannins, flavonoids, phenols, terpenoids, β-caryophyllene, germacrene D, α-pinene, carene, phytol, and linolenic acid in A. africana have been observed to exhibit a very strong anti-inflammatory, antimicrobial, and antioxidant activities which are important processes in wound healing. Indeed, A. africana wound healing ability is furthermore due to the fact that it can effectively reduce wound bleeding, hasten wound contraction, increase the concentration of basic fibroblast growth factor (BFGF) and platelet derived growth factor, and stimulate the haematological parameters, including white and red blood cells, all of which are vital components for the wound healing process. Therefore, these facts may justify why A. africana is used to treat wounds in ethnomedicine.

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The Antimicrobial and Phytochemical Analysis of the Leaves of Aspilia africana on Clinical Isolates

Cited by 6 publications

References 0 publications

Investigation of the antibacterial activities of methanolic and water extracts of Gongronema latifolium, Psidium guajava and Aspilia africana

Investigation of the antibacterial activities of methanolic and water extracts of Gongronema latifolium, Psidium guajava and Aspilia africana

Comparative Hypolipidemic Evaluation of Aframomum Melegueta Seeds and Moringa Oleifera Leaves

The Wound Healing Potential of Aspilia africana (Pers.) C. D. Adams (Asteraceae)

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