The antimicrobial activity of ZnO nanoparticles against Vibrio cholerae : Variation in response depends on biotype

Sarwar, Shamila; Chakraborti, Soumyananda; Bera, Supriyo; Sheikh, Irshad Ali; Hoque, Kazi Mirajul; Chakrabarti, Pinak

doi:10.1016/j.nano.2016.02.006

Cited by 93 publications

(47 citation statements)

References 53 publications

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“…The constitution of the membrane was also found to be responsible for the differential behavior of two biotypes of V. cholera towards ZnO nanaoparticles. 66 It is known that V. cholerae can remodel their membrane architecture frequently just by utilizing exogenous lipids from the environment. 67 This is one of the principle mechanisms (membrane remodeling) through which V. cholerae minimize membrane stress caused by external environment.…”

Section: Discussionmentioning

confidence: 99%

Shape engineering boosts antibacterial activity of chitosan coated mesoporous silica nanoparticle doped with silver: a mechanistic investigation

et al. 2016

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In this study mesoporous silica nanoparticles (MSPs) of different size and shape were developed, and their surface coatings altered to study their differential effect in enhancing the antibacterial activity. In brief, MSPs with three different aspect ratios 1, 2 and 4 were prepared, doped with silver ions and finally coated with the polymer chitosan. Both Gram-positive and Gram-negative bacteria were treated with the MSPs. Results indicated that silver ion doped and chitosan coated MSPs with the aspect ratio 4 (Cht/MSP4:Ag + ) has the highest antimicrobial activity among the prepared series. Further studies revealed that Cht/MSP4:Ag + was most effective against Escherichia coli (E.coli) and least effective against Vibrio cholerae (V.cholerae). To investigate the detailed inhibition mechanism of the MSPs, the interaction of the nanoparticles with E.coli membranes and its intracellular DNA was assessed using various spectroscopic and imaging-based techniques. Furthermore to increase the efficiency of the MSP combinatorial antibacterial strategy was also explored -nanoparticles in combination with kanamycin (antibiotic) was treated against Vibrio Cholerae (V. cholerae). Toxicity screening of these MSPs was conducted against Caco-2 cells, and the results show that the dose used for antibacterial screening is below the limit of toxicity threshold. Our findings show that both shape and surface engineering contributes positively towards bacteria killing, and the newly developed silver ion-doped and chitosan-coated MSP has good potential as an antimicrobial nanomaterial.

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Section: Discussionmentioning

confidence: 99%

Shape engineering boosts antibacterial activity of chitosan coated mesoporous silica nanoparticle doped with silver: a mechanistic investigation

et al. 2016

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“…This was ascribed to the increased uptake of antibiotics and change in the morphology of bacterial cells due to the presence of ZnO NPs. Sarwar et al (2016) examined the antibacterial activity of ZnO NPs against Vibrio cholera (a causative agent of severe watery diarrhea). [71] Depolarization of cell membranes, deformation of cellular architecture, and increased fluidity and protein leakage were found to be induced by the ZnO NPs.…”

Section: Antimicrobial Activity and Its Mechanismmentioning

confidence: 99%

ZnO Nanostructures in Active Antibacterial Food Packaging: Preparation Methods, Antimicrobial Mechanisms, Safety Issues, Future Prospects, and Challenges

Kim

Karthika

Gopinath

et al. 2020

Food Reviews International

211

119

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Packaging of food products is one of the most important stages of the food supply chain. Nano-size materials for packing food substances with appropriate properties result in better packaging performance and longer food shelf-life. In this review, the application of ZnO nano-size in active packaging of foods is discussed to identify gaps in applications for food packaging and safety. First, the crystal structures and morphologies of modified ZnO nanoparticles (ZnO NPs) are presented, and their synergistic effects on antimicrobial activities are discussed. This review also provides an overview of antimicrobial packaging containing ZnO NPs with a focus on preparation methods, antimicrobial mechanisms, and recent progress in packaging applications. The generation of reactive oxygen species (ROS) is the primary antimicrobial mechanism, which can be varied depending on morphology and size. Generally, ZnO NPs can inactivate fungi or Gram-positive and Gram-negative bacteria growth, which reduce the risk of cross-contamination, thereby extending the shelf life of products. Notably, the health concerns and hazards regarding the safety and migration of ZnO NPs application are also elaborated. Unintentional migration, inhalation, skin penetration, and ingestion may result in human health hazards. Therefore, to provide safety regulations, further investigations such as case by case study are recommended. KEYWORDSZinc oxide nanostructure; shelf life; safety; antimicrobial activity; food packaging CONTACT Jongchul Seo

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“…V. cholerae, and other Vibrio species such as V. parahaemolyticus, V. vulnificus, V. fischeri, V. harveyi and V. alginolyticus have been shown to be susceptible to the effect of nanomaterials, such as silver, copper oxide, zinc oxide, boron, titanium dioxide and silver-doped zeolites [7][8][9][10][11][12][13][14][15][16][17][18]. Previous studies revealed an antibacterial effect of zinc-oxide nanoparticles (ZnONP) and silver nanoparticles (AgNPs) against V. cholerae, depends on its lifestyle (planktonic or biofilm); serogroups (O1 or O139) or biotypes (Classical or El Tor); and this effect is also dependent on the nanomaterial composition and concentration [7,10,19,20]. Structural analysis of planktonic cells treated with ZnONPs and AgNPs showed alteration in the bacterial size and shape; in particular, ZnONPs increase the cell membrane fluidity and the reactive oxygen species generation and concomitantly, DNA damage [10,20].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies revealed an antibacterial effect of zinc-oxide nanoparticles (ZnONP) and silver nanoparticles (AgNPs) against V. cholerae, depends on its lifestyle (planktonic or biofilm); serogroups (O1 or O139) or biotypes (Classical or El Tor); and this effect is also dependent on the nanomaterial composition and concentration [7,10,19,20]. Structural analysis of planktonic cells treated with ZnONPs and AgNPs showed alteration in the bacterial size and shape; in particular, ZnONPs increase the cell membrane fluidity and the reactive oxygen species generation and concomitantly, DNA damage [10,20]. It was shown that AgNPs were able to penetrate approximately 40 μm in a thick biofilm after 1 h of exposure, suggesting that biofilm resistance to AgNPs could be at least partially due to retarded silver ion/particle diffusion.…”

Section: Introductionmentioning

confidence: 99%

Effect of antimicrobial nanocomposites on Vibrio cholerae lifestyles: Pellicle biofilm, planktonic and surface-attached biofilm

et al. 2019

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Vibrio cholerae is an important human pathogen causing intestinal disease with a high incidence in developing countries. V. cholerae can switch between planktonic and biofilm lifestyles. Biofilm formation is determinant for transmission, virulence and antibiotic resistance. Due to the enhanced antibiotic resistance observed by bacterial pathogens, antimicrobial nanomaterials have been used to combat infections by stopping bacterial growth and preventing biofilm formation. In this study, the effect of the nanocomposites zeolite-embedded silver (Ag), copper (Cu), or zinc (Zn) nanoparticles (NPs) was evaluated in V. cholerae planktonic cells, and in two biofilm states: pellicle biofilm (PB), formed between air-liquid interphase, and surface-attached biofilm (SB), formed at solid-liquid interfaces. Each nanocomposite type had a distinctive antimicrobial effect altering each V. cholerae lifestyles differently. The ZEO-AgNPs nanocomposite inhibited PB formation at 4 μg/ml, and prevented SB formation and eliminated planktonic cells at 8 μg/ml. In contrast, the nanocomposites ZEO-CuNPs and ZEO-ZnNPs affect V. cholerae viability but did not completely avoid bacterial growth. At transcriptional level, depending on the nanoparticles and biofilm type, nanocomposites modified the relative expression of the vpsL, rbmA and bap1, genes involved in biofilm formation. Furthermore, the relative abundance of the outer membrane proteins OmpT, OmpU, OmpA and OmpW also differs among treatments in PB and SB. This work provides a basis for further study of the nanomaterials effect at structural, genetic and proteomic levels to understand the response mechanisms of V. cholerae against metallic nanoparticles.

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The antimicrobial activity of ZnO nanoparticles against Vibrio cholerae : Variation in response depends on biotype

Cited by 93 publications

References 53 publications

Shape engineering boosts antibacterial activity of chitosan coated mesoporous silica nanoparticle doped with silver: a mechanistic investigation

Shape engineering boosts antibacterial activity of chitosan coated mesoporous silica nanoparticle doped with silver: a mechanistic investigation

ZnO Nanostructures in Active Antibacterial Food Packaging: Preparation Methods, Antimicrobial Mechanisms, Safety Issues, Future Prospects, and Challenges

Effect of antimicrobial nanocomposites on Vibrio cholerae lifestyles: Pellicle biofilm, planktonic and surface-attached biofilm

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