The Antimicrobial Activity of the Essential Oils of Four Juniperus Species Growing Wild in Greece

The chemical composition of the essential oil of Juniperus phoenicea from Algeria was investigated for the first time. The volatile oil was isolated by hydrodistillation in yield 0.80% and was analyzed by GC/FID and GC/MS techniques. One hundred and three constituents, representing 96.0% of the oil was identified, twenty-three being reported for the first time in J. phoenicea oil. The oil was characterized by the presence of high amounts of monoterpene hydrocarbons (62.2%) and by a lower percentage of oxygenated sesquiterpenes (8.8%), of which α−pinene (40.2%), α−phellandrene (14.7%) and elemol (3.9%) were the main compounds. Also the oil was characterized by the presence of three diterpenes constituents; manoyl oxide, abietadiene and cis-totarol amounting to 0.3%. A comparison with the composition of the oils of J. phoenicea (subspecies and varieties) of other countries reported in literature is discussed briefly.

show abstract

“…Thorough investigations of the antimicrobial activity of the oil of this species have been reported (25,26,30).…”

Section: Introductioncontrasting

confidence: 70%

Chemical Composition of the Essential Oil ofJuniperus phoeniceaL. from Algeria

Dob

Dahmane

Chelghoum

2008

Journal of Essential Oil Research

View full text Add to dashboard Cite

show abstract

“…In general, the highest activities of leaf oil were against yeasts (lowest MIC value) except for C. carlspergensis and S. chevallii, which were insensitive to leaf oil. In contrast to Stassi et al (1996), who stated that the antimicrobial activity of J. oxycedrus berry oil was due mainly to its content of terpineol, J. phoenicea berry oil did not contain terpineol, yet it had a high antimicro-bial activity. …”

Section: Antimicrobial Activitymentioning

confidence: 99%

“…Juniper has been used for centuries as a steam inhalant for bronchitis, and to control arthritis. The oil is also irritating to microbes, so much so that it kills many of them (Watt and Breyer-Brandwijk, 1962;Stassi et al, 1996). Traditional formulas are in combination with ginger and dong quai or with goldenseal or with Uva ursi.…”

Section: Introductionmentioning

confidence: 99%

Chemical composition, cytotoxic activity and antimicrobial activity of essential oils of leaves and berries of <i>Juniperus phoenicea</i> l. Grown in Egypt

El-Sawi

Motawae

Ali

2008

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

Hydrodistillation of berries and leaves of Juniperus phoenicea grown in Sinai yielded volatile oils in the yield of 0.36 and 1.96%, respectively. Using gas chromatography/mass spectrometry technique, fifty eight compounds were identified in berry oil representing 99.2% of the oil composition. α-Pinene was the major compound in berry oil (39.30%) followed by sabinene (24.29%). Berry oil composed mainly of monoterpenoids which amounted to 90.53%, of which 72.85% was monoterpene hydrocarbons. The sesquiterpenoids accounted for about 8% of the total oil composition. Leaf oil was composed of about 66 compounds representing 99.16% of the total composition of the oil. α-Pinene was the major constituent of leaf oil at concentration of 38.22%, followed by α -cedrol (31.23%). The monoterpene hydrocarbon was the predominant chemical group (41.29%) followed by the oxygenated sesquiterpenes (32.21%). Both oils showed very high cytotoxic activities against all cell line tested. They showed equal activities against brain (0.6 µg//ml) and cervix (5.0 µg//ml) human cell lines, while berry oil was slightly more active than leaf oil against lung (0.6 and 0.7 µ/ml, respectively), liver (0.7 and 0.9 µg//ml, respectively) and breast human cell lines (0.8 and 1. µg//ml, respectively). The antimicrobial activity and minimum inhibitory concentration (MIC) of leaf and berry oils were also determined. The oils showed high activity against most of the tested strains.

show abstract

“…Also, the yield of EOs from leaves of C. sempervirens L. obtained in this study was lower than that reported by Mazari et al (2010); Elansary et al (2012) and Amri et al (2013), and lower than the yield of EOs from cone of Tunisian C. sempervirens L. which reported by Ben Nouri et al (2015). The yield of EOs isolated from leaves of J. phoenicea L. obtained in this study was lower than that reported by Stasis et al (1996); Mazari et al (2010); Derwich et al (2010) and Amalich et al (2015), but it was relatively similar when compared to that reported by Angioni et al (2003) and Achak et al (2009). 2.…”

Section: Results and Discussion 1 Yield Of The Essential Oilsmentioning

confidence: 99%

Chemical Composition and Antibacterial Activity of Essential Oils Isolated From Leaves of Different Woody Trees Grown In Al-Jabel Al-Akhdar Region, Libya

2016

Alexandria Science Exchange Journal: An International Quarterly

View full text Add to dashboard Cite

The present study reports the variation in the essential oils (EOs) composition from needles and scale leaves of Pinus halepensis, Cupressus sempervirens and Juniperus phoenicea which collected from three different altitudes at Al-Jabel Al-Akhdar area, Libya. The impact of the altitude on the quantity and quality of EOs was studied. In addition, the antibacterial activity was evaluated using microdilution broth assay technique. The results showed that the highest percentages of the EOs yield were found in P. halepensis with 0.41, 0.60, and 0.43% for the altitudes of 125m (I), 391m (II), and 851m (III), respectively, while the lowest percentages of the yield were found in J. phoenicea, with 0.15, 0.07, and 0.18% at altitudes of I, II, and III, respectively. Based on P. halepensis EOs analyses using GC/MS, 35 components have been detected, which represents 89.92, 91.56, and 86.44% for the altitudes of I, II and III, respectively. Furthermore, high percentages from the components of α-pinene, β-pinene, α-terpineol and caryophyllene were identified at the three tested altitudes. For C. sempervirens EOs, 33 components were identified representing 84.94, 93.37 and 99.48% for levels I, II and III, respectively. The highest percentages of the EOs components in this species were α-pinene, terpinen-4-ol and α-terpiny acetate at the three tested elevations. However, the detected main components in J. phoenecea EOs were α-pinene, α-myrcene, α-terpinyl acetate and γ-terpinene. The highest antibacterial activity was observed for the oils from P. halepensisat and J. phoenicea at altitude I against the Agrobacterium tumefaciens, Erwinia carotovora, Corynebacterium fascians and Pseudomonas solanacearum. While the EOs extracted from scale leaves of C. sempervirens obtained from trees growing at level II were the most active against the tested bacteria. The results of this work revealed the impacts of the environmental conditions on the EOs composition which affected significantly in its quantitative and qualitative performances. Consequently, their biological activities were varied considerably. Moreover, the obtained data offer the opportunity to choose EOs with preferential compound for pharmaceutical, pesticides, perfume and food industries in such important region in Libya.

show abstract

The Antimicrobial Activity of the Essential Oils of Four Juniperus Species Growing Wild in Greece

Cited by 19 publications

References 0 publications

Chemical Composition of the Essential Oil ofJuniperus phoeniceaL. from Algeria

Chemical Composition of the Essential Oil ofJuniperus phoeniceaL. from Algeria

Chemical composition, cytotoxic activity and antimicrobial activity of essential oils of leaves and berries of <i>Juniperus phoenicea</i> l. Grown in Egypt

Chemical Composition and Antibacterial Activity of Essential Oils Isolated From Leaves of Different Woody Trees Grown In Al-Jabel Al-Akhdar Region, Libya

Contact Info

Product

Resources

About