The antimicrobial activity of hydrophobic essential oil constituents acting alone or in combined processes of food preservation

Ait-Ouazzou, Abdenour; Cherrat, Lamia; Espina, Laura; Lorán, Susana; Rota, C.; Pagán, Rafael

doi:10.1016/j.ifset.2011.04.004

Cited by 182 publications

(131 citation statements)

References 30 publications

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“…The antibacterial activity of the C. rhamnifolioides essential oil in the present study can be attributed to the presence of such compounds as 1.8-cineol, α-pinene, felandreno-1 and p-cymene, which have been previously reported to possess antimicrobial properties (OKE et al, 2009;AIT-OUAZZOU et al, 2011). It is believed that monoterpene and sesquiterpene compounds, such as those found in C. rhamnifolioides essential oil, can accumulate in the bacterial membrane and cause a loss of integrity, leakage of the cytoplasmic contents, disruption of the proton motive force, lysis and cell death (BEN ARFA et al, 2006;SANTIESTEBAN-LÓPEZ;PALOU;LÓPEZ-MALO, 2007).…”

Section: Antimicrobial Activitysupporting

confidence: 54%

Chemical composition and antibacterial activity of essential oil of a Croton rhamnifolioides leaves Pax & Hoffm

et al. 2013

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This study describes the chemical composition and antimicrobial activity of an essential oil from the leaves of Croton rhamnifolioides Pax&Hoffm., an aromatic plant widely distributed in northeastern Brazil and commonly used in folk medicine. Eight components were identified: 1.8-cineole (46.32%) as the major compound, followed by 1-felandreno (16.70%), p-cymene (10.21%), sabinene (8.14%) and trans-caryophyllene (4.81%). The essential oil exhibited antibacterial activity against Aeromonas hydrophila, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis and Staphylococcus aureus, with minimum inhibitory concentrations and minimum bactericidal concentrations ranging from 2.5 to 20 and 5 to 40 µL.mL -1 , respectively. In addition, the essential oil also affected the cellular viability of these bacteria. The results suggest the need for research to establish the potential uses of this essential oil as a natural antimicrobial in the food industry. Key words: Croton rhamnifolioides, essential oil, antimicrobial activity, chemical composition ResumoO presente estudo descreve a composição química e a atividade antimicrobiana do óleo essencial das folhas de Croton rhamnifolioides Pax & Hoffm, uma planta aromática amplamente distribuída no Nordeste do Brasil e largamente utilizada na medicina popular. Cromatografia gasosa -espectrometria de massa foi utilizada para determinar sua composição. Oito componentes foram identificados, sendo 1,8-cineol (46,32%) o composto majoritário, seguido por 1-felandreno (16,70%), p-cimeno (10,21%), sabineno (8,14%) e trans-cariofileno (4,81%). O óleo essencial apresentou atividade antibacteriana contra Aeromonas hydrophila, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis e Staphylococcus aureus com valores de concentração inibitória mínima (CIM) e concentração bactericida mínima (CBM) entre 2,5 -20 μL/mL e 5 -40 μL/mL, respectivamente. Além disso, o óleo essencial também afetou a viabilidade celular dessas bactérias. Os resultados sugerem a necessidade de estudos para estabelecer o uso potencial deste óleo essencial como antimicrobiano natural na indústria de alimentos. Palavras-chave: Croton rhamnifolioides, óleo essencial, atividade antimicrobiana, composição química

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Section: Antimicrobial Activitysupporting

confidence: 54%

Chemical composition and antibacterial activity of essential oil of a Croton rhamnifolioides leaves Pax & Hoffm

et al. 2013

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“…Parámetros estimados del modelo de Weibull propuesto para describir la inactivación de E. coli en jugo de zanahoria por diferentes concentraciones de carvacrol emulsionado (CA-E) y la combinación de CA-E con acidificación (pH 4,5). This synergistic effect of carvacrol combined with acidification was also described by Ait-Ouazzou et al (2011), who evaluated the inactivation of E. coli by different constituents of essential oils, such as carvacrol, at pH 7.0 and 4.0 in citratephosphate buffer. They observed that 0.2 µL/L of carvacrol at pH 7.0 resulted in approximately 1.8 and 2.8 log cycles reduction of E. coli after 6 and 24 h of storage, respectively.…”

Section: Combined Effect Of Ca-e and Acidification In Carrot Juicesupporting

confidence: 52%

“…It is well known that pH level can modify not only microbial resistance to physical and chemical agents but also the mechanism of action of synthetic antimicrobials (Espina et al, 2012). However, there is still little information available about the effect of pH on natural antimicrobial activity and the results are variable among studies (Ait-Ouazzou et al, 2011;Somolinos, García, Mackey & Pagán, 2010).…”

Section: Combined Effect Of Ca-e and Acidification In Carrot Juicementioning

confidence: 99%

Effect of emulsification on the antimicrobial activity of carvacrol

Char

Cisternas

Pérez

et al. 2015

CyTA - Journal of Food

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This work focuses on the emulsification of carvacrol for its incorporation into juices with the aim of retaining antimicrobial activity while enhancing the stability of the oil in aqueous systems. Carvacrol was emulsified (CA-E) using capsul ® (1:2 emulsion) and its antimicrobial activity was determined on Escherichia coli and Lactobacillus plantarum. The combined effect of CA-E and pH reduction to 4.5 was assessed on different juices. The sensitivity of L. plantarum to carvacrol was not affected by emulsification, whereas E. coli presented higher minimal inhibitory concentrations. Combined treatments improved the effect: 0.5 µL/mL CA-E increased from 0.2 to 2.1 log reductions of E. coli. Carvacrol emulsion (1.0 µL/mL) successfully inactivated E. coli in apple and orange juices, attaining undetectable levels (<1 log CFU/mL). The efficacy of carvacrol emulsion was improved by acidification; therefore, its incorporation at low doses in acidic foods may be a useful alternative for multiple applications.Keywords: natural antimicrobials; emulsion; carrot juice; Escherichia coli; Lactobacillus plantarum Este trabajo se basa en la emulsificación del carvacrol para su incorporación a jugos con el objetivo de retener su actividad antimicrobiana y mejorar la estabilidad del aceite en sistemas acuosos. El carvacrol se emulsificó (CA-E) utilizando capsul ® (emulsión1:2) y la actividad antimicrobiana se determinó sobre Escherichia coli y Lactobacillus plantarum. El efecto combinado de CA-E y la reducción del pH a 4,5 se evaluó en diferentes jugos. La emulsión no afectó la sensibilidad de L. plantarum al carvacrol; sin embargo, E. coli presentó mayor concentración mínima inhibitoria. Los tratamientos combinados incrementaron el efecto, 0,5 µL/mL CA-E aumentó desde 0,2 a 2,1 reducciones log de E. coli. La emulsión de carvacrol (1,0 µL/mL) inactivó exitosamente a E. coli en jugos de manzana y naranja, alcanzando niveles indetectables (<1 log UFC/mL). La eficacia de la emulsión de carvacrol aumentó por la acidificación, permitiendo su uso en bajas dosis en alimentos ácidos, constituyendo una alternativa para múltiples aplicaciones en alimentos.

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“…This result was attributed to the presence of 1,8-cineol, the major constituent. Other studies show that this compound when isolated possesses antimicrobial activity (OKE et al, 2009;ALT-OVAZZOU et al, 2011). However, the EOC did not present antibacterial activity via direct contact in microdilution trials, which can be attributed to chemical differences in its composition when compared to the previous study, such as the difficulty of terpene penetration in the presence of DMSO (BOTREL et al, 2010).…”

Section: Resultsmentioning

confidence: 83%

Chemical composition, antibacterial and modulatory action of the essential oil of Croton rhamnifolioides leaves Pax and Hoffman

Vidal¹,

Oliveira-Tintino²,

Tintino³

et al. 2016

Biosci. J.

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ABSTRACT:The species Croton rhamnifolioides is a shrub popularly known as "quebra faca", utilized in teas or infusions for the treatment of gastrointestinal disorders as ulcers, gastric discomfort, vomiting and hemorrhagic diarrhea. This work has as its main objective to evaluate the chemical composition of the essential oil of the Croton rhamnifolioides leaves (EOC), antibacterial activity and modulatory activity of antibiotic resistance, against bacterial strains of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa and, and antifungal activity against Candida albicans, Candida krusei and Candida tropicalis. The chemical composition was determined through chromatography with a gas phase coupled with mass spectrometry (CG/MS), whereas antimicrobial activity and modulation were determined by microdilution assay. The chemical composition of the oil revealed 21 compounds, with the major compounds being spathulenol (22.46%) and 1,8-cineol (18.32%). The antimicrobial activity was not significant with MIC>1024 µg/mL, however, was observed a synergistic interference when essential oil associated with the antibiotics aminoglycoside and β-lactam, and the antifungal polyene.

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The antimicrobial activity of hydrophobic essential oil constituents acting alone or in combined processes of food preservation

Cited by 182 publications

References 30 publications

Chemical composition and antibacterial activity of essential oil of a Croton rhamnifolioides leaves Pax & Hoffm

Chemical composition and antibacterial activity of essential oil of a Croton rhamnifolioides leaves Pax & Hoffm

Effect of emulsification on the antimicrobial activity of carvacrol

Chemical composition, antibacterial and modulatory action of the essential oil of Croton rhamnifolioides leaves Pax and Hoffman

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