The Antiglobulin (Coombs) Test in Brucellosis

Wilson, Michael M.; Merrifield, E.V.O

doi:10.1016/s0140-6736(51)91875-2

Cited by 41 publications

(11 citation statements)

References 8 publications

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“…Subfractions (II 1, 2 and II 3) of human gamma globulin contain both complete and incomplete enterobacterial antibodies. 6. The possible implications of the findings, with particular reference to the clinical use of human gamma globulin, are discussed.…”

Section: Discussionmentioning

confidence: 93%

Study on Antibodies Against Enteric Pathogens in Human Gamma Globulin

Neter¹,

Drislane²,

Harris³

et al. 1959

Am J Public Health Nations Health

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Section: Discussionmentioning

confidence: 93%

Study on Antibodies Against Enteric Pathogens in Human Gamma Globulin

Neter¹,

Drislane²,

Harris³

et al. 1959

Am J Public Health Nations Health

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“…Chronic brucellosis is a poorly defined clinical state and, when suspected, laboratory confirmation is frequently requested. Kerr et al (1968) described an AHG test based on that of Wilson & Merrifield (1951) and advocated its use in the diagnosis of chronic brucellosis. They stated that a positive AHG test when accompanied by CF antibodies and taken in conjunction with clinical evidence may support a diagnosis of chronic brucellosis.…”

Section: Resultsmentioning

confidence: 99%

Serum antibody response in acute brucellosis

Farrell¹,

Robertson²,

Hinchliffe³

1975

J. Hyg.

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“…The latter method has been recommended by Jones and Wilson (29) and by Wilson and Merrifield (30) for increasing the sensitivity of Brucella agglutination tests, eliminating prozones and overcoming blocking antibodies. We have confirmed their findings with regard to the Coombs method (Figure 4).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Coombs Test With Other Methods for Brucella Agglutinins in Human Serum 1

Hall¹,

Manion²

1953

J. Clin. Invest.

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During the past year we have compared several techniques for testing human sera for Brucella agglutinins. These have included a tube dilution method, the rapid slide method (Huddleson) and a rapid slide or screening test designed by Castaneda (1). In addition, a blocking test and a modification of the indirect Coombs test were investigated. High speed centrifugation of Brucellaserum mixtures was also used to increase the titer of Brucella agglutinins.Several unsuccessful attempts also were made to adapt the Middlebrook-Dubos (2) hemagglutination method to the detection of antibodies against Brucella. In addition, paper chromatograms were made in attempts to differentiate specific from non-specific Brucella agglutinins by the method of Hess and Roepke (3).Sera from 104 adults were studied. Of these subjects, 27 had brucellosis proved by culture, 28 had presumptive evidence of brucellosis, and 49 had no evidence of infection with Brucella and were classed as "normal" controls. METHODSVenous blood was obtained aseptically and allowed to clot in a sterile glass tube in a refrigerator. The serum was separated within a few hours. It was not heatinactivated but was tested immediately or stored in a deep-freeze cabinet at O°C. until tested.Tube dilution agglutination method. The agglutinin titer was determined by a modification of the method of Fitch, Donham, Bishop, and Boyd (4). The serum was diluted two-fold serially from 1: 10 to 1: 2,560 with 0.85 per cent saline solution. Brucella abortus antigen (0.5 ml.) was added to 0.5 ml. of saline (control) and 0.5 ml. of each dilution of the serum in separate tubes.' The

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The Antiglobulin (Coombs) Test in Brucellosis

Cited by 41 publications

References 8 publications

Study on Antibodies Against Enteric Pathogens in Human Gamma Globulin

Study on Antibodies Against Enteric Pathogens in Human Gamma Globulin

Serum antibody response in acute brucellosis

Comparison of the Coombs Test With Other Methods for Brucella Agglutinins in Human Serum 1

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