The antigenicity of the outer membrane proteins of Brucella

Bulashev, Aitbay; Сұраншиев, Ж; Zhumalin, Aibek; Tursunov, Kanat

doi:10.11134/btp.1.2016.3

Cited by 7 publications

(8 citation statements)

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“…At the same time, outer membrane proteins are a very valuable component of the cell wall, consisting of diagnostically important proteins that have the necessary specificity and can be used in serological diagnostic tests (Ricard et al 2022). Thus, when developing an ELISA protocol, it is advisable to use outer membrane proteins as antigens (Sova and Kusaykin 2006;Bulashev et al 2016).…”

Section: Discussionmentioning

confidence: 99%

The Use of Various Tests for the Serological Diagnosis of Salmonella Abortion in Horses in Kazakhstan

2023

Int J Vet Sci

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Abortions of horses caused by Salmonella cause great economic damage to horse breeding farms and private farmsteads in Kazakhstan. This infection is primarily diagnosed through bacteriology, but it is highly dependent on the state of the material under study. It is also time-consuming and lacks sensitivity. Moreover, PCR analysis is used to identify and differentiate the causative agent of Salmonella abortion; the main disadvantage of this is the high cost of equipment and test systems. At the same time, in our country, the effective and affordable method of enzyme-linked immunosorbent assay (ELISA) for serological diagnosis and monitoring of this infection is not used. The aim of this work is to show the use of ELISA based on outer membrane proteins (OMPs) for the serological diagnosis of Salmonella abortion in horses. Self-produced OMPs of S. enterica abortus egui were used in ELISA. Serum samples were taken from aborted mares and contact animals. According to the results of the study of blood sera of mares, the presence of specific antibodies was established in 20-43% of cases. The detection of antibodies indicates that the animal has had a disease or is a carrier of the causative agent of the infection, and, in general, an unfavourable situation for this infection in the surveyed farm. The use of ELISA and latex agglutination tests (LAT) makes it possible to determine the presence of specific antibodies in the serum of unvaccinated animals and can be used to monitor the epizootic situation for this infection.

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Section: Discussionmentioning

confidence: 99%

The Use of Various Tests for the Serological Diagnosis of Salmonella Abortion in Horses in Kazakhstan

2023

Int J Vet Sci

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“…Apparently, Omp19 exposure may be different in live versus inactivated bacteria; as such, the results obtained from rabbits immunized with killed Brucella might be different from those obtained from experimentally infected cattle. We have previously described the strong antigenic properties of a 19 kDa protein detected on Western blots of soluble protein preparations from B. abortus and B. melitensis probed with serum from a cow that was naturally infected with brucellosis [ 34 ]. These data suggest that Omp19 may be more antigenic than the other Brucella Group 3 antigens.…”

Section: Discussionmentioning

confidence: 99%

Use of recombinant Brucella outer membrane proteins 19, 25, and 31 for serodiagnosis of bovine brucellosis

et al. 2020

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Background and Aim: Brucellosis remains one of the most common zoonoses. The current anti-brucellosis measures are largely deemed ineffective due to a lack of specificity of conventional serological tests. This study evaluated the use of Brucella outer membrane protein (Omp)19 for serodiagnostic testing. Materials and Methods: The antigenicity of recombinant Brucella Omp19, Omp25, and Omp31 was examined in serum samples from mice and rabbits immunized with Omp19 or Brucella abortus 19 whole cell (WC) and 12 and 152 cows experimentally or naturally infected with brucellosis, respectively. Serum samples were collected from 151 cows that were vaccinated with B. abortus 19 and 12 unvaccinated heifers that were maintained on a brucellosis-free farm. Results: Immunization with Omp19 resulted in antibody production in mice after a single injection without the use of adjuvant. Serum antibodies obtained from rabbits immunized with inactivated B. abortus strain 19 WC targeted Omps by enzyme-linked immunosorbent assay (ELISA) and Western blot. Antibodies targeting Omp19 were identified in all B. abortus strain 544 experimentally infected cows at day 14 post-inoculation (p.i.); Omp25 was detected by ELISA at day 28 p.i., while an ELISA targeting Omp31 was negative for 25% of cows at this time point. Omp19 and Omp25 were readily detected by sera from cows from a new epizootic focus. Antibodies recognizing Omps were also detected in >50% of the animals maintained in a brucellosis-free herd at 10 months after vaccination. Conclusion: Brucella Omp19 in combination with Omp25 and Omp31 may be utilized as target antigens in an ELISA designed for serological testing of unvaccinated cattle.

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“…Brucella rOmp25 and rOmp31 used in this study were obtained in our earlier research (5). The extraction of CSP from Brucella cells was carried out according to the method of Tabatabai and Deyoe (35).…”

Section: Methodsmentioning

confidence: 99%

Using combined recombinant protein in the diagnosis of bovine brucellosis

Bulashev¹,

Jakubowski²,

Mukantayev³

et al. 2018

Medycyna Weterynaryjna

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SummaryThe aim of this research was to obtain a combined recombinant protein consisting of immunodominant regions of Brucella outer membrane proteins (Omps) with the molecular weights of 25 kDa (Omp25) and 31 kDa (Omp31). The search for candidate proteins was carried out using NCBI PubMed and NCBI GenBank databases. The two most immunodominant regions of Omps were selected. The first region was in a position from 48 to 83 amino acids of Omp31, while the second one was in the position from 180 to 224 amino acids of Omp25. This combined sequence was designated as OmpBm-Ba. The pET32 vector was used for cloning and the expression of the combined recombinant protein in E. coli BL21(DE3). The antigenicity of recombinant OmpBm-Ba (rOmpBm-Ba) as compared with rOmp25 and rOmp31 has been tested on the sera of 109 cattle with positive results to brucellosis according to classical serological tests. The rOmpBm-Ba had a higher antigenicity than the single ones, since it confirmed the presence of Brucella-antibody in all serum samples with positive results of i-ELISA/rOmp25 and/or i-ELISA/rOmp31, as well as additionally revealing 7.3% and 31.2% seropositive animals, respectively. A comparative study of the diagnostic value of i-ELISA/rOmpBm-Ba and conventional tests on blood sera of 24 cattle subjected to a bacteriological examination at slaughter showed a higher reliability of enzyme immunoassay. Further research is necessary to get a more objective evaluation of the diagnostic accuracy of Brucella rOmpBm-Ba by challenging laboratory animals.

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The antigenicity of the outer membrane proteins of Brucella

Cited by 7 publications

References 0 publications

The Use of Various Tests for the Serological Diagnosis of Salmonella Abortion in Horses in Kazakhstan

The Use of Various Tests for the Serological Diagnosis of Salmonella Abortion in Horses in Kazakhstan

Use of recombinant Brucella outer membrane proteins 19, 25, and 31 for serodiagnosis of bovine brucellosis

Using combined recombinant protein in the diagnosis of bovine brucellosis

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