The antifungal mechanisms of plant volatile compound 1-octanol against Aspergillus flavus growth

Qin, Yu-Liang; Zhang, Shuai-Bing; Lv, Yangyong; Zhai, Huan-Chen; Hu, Yuansen; Cai, Jing

doi:10.1007/s00253-022-12049-z

Cited by 25 publications

(5 citation statements)

References 66 publications

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“…The conductivity of the pathogenic culture with different treatment time were measured to determine the cell membrane leakage of B. sorokiniana ( Shao et al, 2015 ; Lin et al, 2018 ). The ergosterol content in mycelial plasma membrane of B. sorokiniana treated with CF (1:1 v/v) for 48 h was determined as previously described by Qin et al (2022) .…”

Section: Methodsmentioning

confidence: 99%

Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana

Luan

Cui

et al. 2023

Front. Microbiol.

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IntroductionBipolaris sorokiniana is the popular pathogenic fungi fungus which lead to common root rot and leaf spot on wheat. Generally, chemical fungicides are used to control diseases. However, the environmental pollution resulting from fungicides should not be ignored. It is important to study the mode of antagonistic action between biocontrol microbes and plant pathogens to design efficient biocontrol strategies.ResultsAn antagonistic bacterium DB2 was isolated and identified as Bacillus amyloliquefaciens. The inhibition rate of cell-free culture filtrate (CF, 20%, v/v) of DB2 against B. sorokiniana reached 92.67%. Light microscopy and scanning electron microscopy (SEM) showed that the CF significantly altered the mycelial morphology of B. sorokiniana and disrupted cellular integrity. Fluorescence microscopy showed that culture filtrate destroyed mycelial cell membrane integrity, decreased the mitochondrial transmembrane potential, induced reactive oxygen species (ROS) accumulation, and nuclear damage which caused cell death in B. sorokiniana. Moreover, the strain exhibited considerable production of protease and amylase, and showed a significant siderophore and indole-3-acetic acid (IAA) production. In the detached leaves and potted plants control assay, B. amyloliquefacien DB2 had remarkable inhibition activity against B. sorokiniana and the pot control efficacy was 75.22%. Furthermore, DB2 suspension had a significant promotion for wheat seedlings growth.ConclusionB. amyloliquefaciens DB2 can be taken as a potential biocontrol agent to inhibit B. sorokiniana on wheat and promote wheat growth.

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Section: Methodsmentioning

confidence: 99%

Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana

Luan

Cui

et al. 2023

Front. Microbiol.

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“…The tubes were then incubated at 28°C for 12 hours, with a treatment without CF being used as a control. The treated mycelium was washed twice with PBS and then samples were prepared to observe the microscopic morphology under a scanning electron microscope (SEM, HITACHI SU8010), based on previous reports (Qin et al 2022).…”

Section: Inhibitory Effect Of Jk-11 Fermentation Supernatant On Bipol...mentioning

confidence: 99%

“…The ergosterol content in the cell membrane of B. sorokiniana mycelia was analysed after treatment with 20% JK-11CF for 24 hours, according to the study by Qin et al (Qin et al 2022). B. sorokiniana was rst inoculated in PDB medium and then allowed to cultivate for a period of 72 hours.…”

Section: Effect Of Jk-11 Cf On the Cell Membrane Of Bipolaris Sorokin...mentioning

confidence: 99%

Biocontrol potential and mechanism of action of Bacillus tequilensis JK-11 against Bipolaris sorokiniana.

kang,

Fan,

Zhang

et al. 2023

Preprint

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Root rot of wheat, caused by the pathogen Bipolaris sorokiniana, has emerged as a major concern for wheat yield damage and food security in central China. Given their environmentally friendly nature, biological control methods are becoming increasingly popular. We conducted a study to assess the antagonistic effect of Bacillus tequilensis JK-11 on B. sorokiniana. Spore germination and mycelial dry weight of B. sorokiniana decreased by 84.9% and 80.63% after JK-11 culture filtrate treatment with a concentration of 20%. SEM observed significant damage to the microscopic morphology of B. sorokiniana cells, and LSCM observed significant apoptosis of B. sorokiniana mycelial cells in the treated group, in addition to changes in ergosterol and electrical conductivity suggesting elevated mycelial cell membrane permeability. In contrast to the untreated group, CF-treated B. sorokiniana cells showed significant DNA and mitochondrial damage and excessive accumulation of reactive oxygen species. The activities of antioxidant enzymes and key enzymes involved in metabolic processes were significantly impaired in mycelial cells treated with different concentrations of CF. Wheat potting experiments using JK-11 revealed its ability to stabilize wheat root colonization for a certain period. Moreover, the bacterial suspension of JK-11 had a significant impact on wheat root rot prevention post-irrigation. The study results suggest that JK-11 strain of B. tequilensis exhibits great potential for controlling wheat root rot and offers a fresh approach to wheat management.

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“…Apple (Malus domestica) is an important fruit that provides bene cial nutrients of vitamins, polyphenols, and dietary ber for human health. China is the leading country for apple production worldwide, devoting approximately half of the total apple production (Zhang et al, 2022). In many temperate areas of China, apples are seasonally harvested from September to November and stored in cold conditions for long-term trade and consumption.…”

Section: Introductionmentioning

confidence: 99%

Salicylic acid promoted apple metabolic responses against Penicillium expansum infection

Zhang

et al. 2023

Preprint

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Blue mold caused by Penicillium expansum (P. expansum) infection results in severe postharvest deterioration of apples. Salicylic acid (SA) is an effective elicitor that promotes fruit resistance. However, the metabolic mechanism of P. expansum infection of apples and the SA-mediated metabolic responses are still unknown. In this study, the metabolic changes during apple P. expansum infection and SA-mediated disease resistance were explored by performing ultra-performance liquid chromatography and quadrupole-time of flight mass spectrometry. A total of 472 different metabolites were identified between samples, and the correlated metabolic pathways were revealed by bioinformatics analysis. The upregulation of the tricarboxylic acid (TCA) cycle, galactose metabolism, and starch and sucrose metabolism reflected energy conversion for P. expansum invasion and fruit disease resistance. Changes in glyoxylate and dicarboxylate metabolism and carbapenem biosynthesis reflected the biosynthesis of virulence factors and secondary metabolites for fungal infection. Metabolic pathways related to apple natural disease resistance mainly included the upregulation of secondary metabolite biosynthesis and sphingolipid metabolism. SA promoted the TCA cycle, reactive oxygen metabolism and secondary metabolite biosynthesis of apples for disease resistance. This study improved the understanding of the pathogenic mechanism of P. expansum infection of apples and the metabolic processes for SA-mediated disease resistance.

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The antifungal mechanisms of plant volatile compound 1-octanol against Aspergillus flavus growth

Cited by 25 publications

References 66 publications

Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana

Biocontrol potential and action mechanism of Bacillus amyloliquefaciens DB2 on Bipolaris sorokiniana

Biocontrol potential and mechanism of action of Bacillus tequilensis JK-11 against Bipolaris sorokiniana.

Salicylic acid promoted apple metabolic responses against Penicillium expansum infection

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