The antifungal mechanism of konjac flying powder extract and its active compounds against wood decay fungi

Bi, Zhenju; Zhao, Yan; Morrell, Jeffrey J.; Lei, Yafang; Li, Yan

doi:10.1016/j.indcrop.2021.113406

Cited by 28 publications

(10 citation statements)

References 21 publications

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“…Moreover, the imbalance of electrolytes inside and outside the cell resulted in the inhibition of mycelial growth. It has been shown that intact cell membranes were critical for controlling the ingress and egress of substances and disruption often resulted in the leakage of small molecules (Na + , K + ), intracellular proteins, and nucleic acids, out of the cell, resulting in loss of cellular function. , Our study is in agreement with the mechanism of action of the dichloromethane extract from Streptomyces libani and natural products eugenol and citral on the cell membranes. , …”

Section: Discussionsupporting

confidence: 88%

“…It has been shown that intact cell membranes were critical for controlling the ingress and egress of substances and disruption often resulted in the leakage of small molecules (Na + , K + ), intracellular proteins, and nucleic acids, out of the cell, resulting in loss of cellular function. 57,58 Our study is in agreement with the mechanism of action of the dichloromethane extract from Streptomyces libani and natural products eugenol and citral on the cell membranes. 59,60 Ergosterol is the main component of the cell membrane, which plays an important role in the integrity of the cell membrane, the activity of membrane-binding enzymes, the fluidity of membrane, and the transportation of cell material.…”

Section: ■ Discussionsupporting

confidence: 86%

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Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Zhao

Wang

et al. 2022

ACS Omega

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Rice sheath blight (RSB) caused by Rhizoctonia solani is one of the most destructive diseases of rice (Oryza sativa). Although chemical fungicides are the most important control methods, their long-term unreasonable application has brought about problems such as environmental pollution, food risks, and non-target poisoning. Therefore, considering the extraction of fungistatic substances from plants may be an alternative in the future. In this study, we found that the Moutan cortex ethanol extract has excellent antifungal activity against R. solani, with a 100% inhibition rate at 1000 μg/mL, which aroused our great exploration interest. In-depth exploration found that the antifungal active ingredients of M. cortex were mainly concentrated in the petroleum ether extract of the M. cortex ethanol extract, which still maintained a 100% inhibition rate with 250 μg/mL, and its effective medium concentration (EC 50 ) was 145.33 μg/mL against R. solani. Through the measurement of extracellular relative conductivity and OD 260 , the petroleum ether extract induced leakage of intracellular electrolytes and nucleic acids, indicating that the cell membrane was ruined. Therefore, we preliminarily determined that the cell membrane may be the target of the petroleum ether extract. Moreover, we found that petroleum ether extract reduced the content of ergosterol, a component of the cell membrane, which may be one of the reasons for the cell membrane destruction. Furthermore, the increase of MDA content would lead to membrane lipid peroxidation, further aggravating membrane damage, resulting in increased membrane permeability. Also, the destruction of the cell membrane was observed by the phenomenon of the mycelium being transparent and broken. In conclusion, this is the first report of the M. cortex petroleum ether extract exhibiting excellent antifungal activity against R. solani. The effect of the M. cortex petroleum ether extract on R. solani may be on the cell membrane, inducing the disorder of intracellular substances and metabolism, which may be one of the antifungal mechanisms against R. solani.

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Section: Discussionsupporting

confidence: 88%

Section: ■ Discussionsupporting

confidence: 86%

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Zhao

Wang

et al. 2022

ACS Omega

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“…The PBS-containing mycelia were amended with 0.05/mL of the 0.5:1.0, 1.0:1.0, 1.5:1.0, 2.0:1.0, 2.5:1.0 and 3.0:1.0 emulsion and incubated at 30 °C for 6 h, and then, the resulting culture was centrifuged at 10,000 rpm for 5 min. Absorbance of the resulting supernatant was measured at 260 nm (OD 260 nm ) to assess the effect of varying levels of the emulsion on nucleic acid levels in the media (Bi et al 2021). The results served as a measure of the effects of the mixture on cell membrane function (i.e.…”

Section: Fungal Cell Membrane Integrity: Nucleic Acid Leakagementioning

confidence: 99%

Improvement of mould resistance of wood with cinnamaldehyde chitosan emulsion

Fang

Gao

et al. 2021

Wood Sci Technol

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Mould fungi can grow on virtually any substrate and are particularly common on cellulosic materials such as wood. Fungicides can limit the risk of mould attack, but increasing concerns about pesticide use have encouraged a search for more natural protection methods. Cinnamaldehyde is a promising antifungal candidate, but has not been fully effective. In this study, chitosan and cinnamaldehyde were used to prepare an emulsion to reduce the volatility of cinnamaldehyde, and the antimould performance of the emulsion-treated wood was evaluated. The ability of cinnamaldehyde chitosan emulsion was assessed for inhibiting mould growth as well as its effect on membrane function and hyphal morphology of Aspergillus niger. Cinnamaldehyde chitosan emulsion can reduce the amount of free cinnamaldehyde, thereby increasing the retention rate of cinnamaldehyde. The control ability of wood treated with 3.0:1.0 molar ratio of aldehyde to amino group in cinnamaldehyde chitosan emulsion against A. niger was 95.8%. Mould growth was limited in the presence of the emulsion. The treatment appeared to disrupt cell membrane function as evidenced by increased levels of extracellular proteins and nucleic acids. The results suggest that the cinnamaldehyde chitosan emulsion improved the stability of cinnamaldehyde in aqueous solution as well as effectively protected wood from mould.

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“…The components of wood extract are diverse, and different components have different properties ( Rudman and Da Costa, 1959 ; Kirker et al, 2013 ; Ateş et al, 2015 ; Wang et al, 2020 ). For example, the chelating properties of metals in wood extract can significantly reduce fungal activity, and the activities of type II peroxidase and copper-dependent laccase in white-rot fungi have been demonstrated to be susceptible to metal interference in wood extract ( Pollegioni et al, 2015 ; El-Hefny et al, 2020 ; Bi et al, 2021 ; Vek et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Anti-fungal Activity of Dalbergia retusa Extract on Gloeophyllum trabeum

Wang

et al. 2022

Front. Plant Sci.

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Hongmu is a type of material with strong corrosion resistance, and its extract has wood preservative properties in a variety of environmental settings. Herein, the processing residue of Dalbergia retusa was used to obtain an ethanol-extract, whose anti-fungal properties and mechanism was investigated using multi-omics principles and gas chromatography-mass spectrometry (GC-MS). The results show that D. retusa extract had a strong inhibitory effect on decaying fungus, and the inhibitory effect was dose dependent. High-throughput sequencing detected a total of 11,755 genes for transcriptome comparison. A total of 390 genes were differentially expressed, with 69 up-regulated and 321 down-regulated genes, indicating that D. retusa extract can significantly affect metabolic processes in decaying fungus. GC-MS results revealed that D. retusa extract was rich in phenols, ketones, amines, and aromatic compounds, which are likely to contribute to the excellent synergy between anti-fungal properties and anti-fungal activity (anti-fungal ability and active ingredients). In summary, this study describes the anti-fungal components in D. retusa extract, and our results provide a foundation for the study of their mechanism of action in this tree species.

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The antifungal mechanism of konjac flying powder extract and its active compounds against wood decay fungi

Cited by 28 publications

References 21 publications

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Anti-Fungal Activity of Moutan cortex Extracts against Rice Sheath Blight (Rhizoctonia solani) and Its Action on the Pathogen’s Cell Membrane

Improvement of mould resistance of wood with cinnamaldehyde chitosan emulsion

Anti-fungal Activity of Dalbergia retusa Extract on Gloeophyllum trabeum

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