The Antifungal Effect of Pyroligneous Acid on the Phytopathogenic Fungus Botrytis cinerea

Abstract

In recent years, climate change has intensified harsh periods of rain alternating with periods of drought, leading to an increase in the presence of phytopathogenic fungi. In this study, we want to analyse the antifungal properties of pyroligneous acid against the fungal phytopathogen Botrytis cinerea. Through the inhibition test, we observed that the application of different dilutions of pyroligneous acid rarefied the growth of the fungal mycelium. Furthermore, we have seen through the metabolic profile that B. cinerea is not able to use pyroligneous acid as a resource or even grow in close contact with this resource. Moreover, we observed that the pre-incubation of the fungus in pyroligneous acid leads to a reduction in biomass production. These results give us hope for the possible use of this natural substance as a possible substance to protect plantations from pathogen attacks.

Comprehensive antifungal investigation of natural plant extracts against Neosartorya spp. (Aspergillus spp.) of agriculturally significant microbiological contaminants and shaping their metabolic profile

“…To test the effect of the inhibitory properties of these extracts on growth, Petri plates with PDA medium (BioMaxima S.A., Lublin, Poland) were inoculated with 300 µL of a Neosartorya spp. water suspension, as described in Pertile et al 41 . To ensure a uniform number of cells inside the inoculum, fungal material included ascospores with mycelium collected from the previous 10 days old culture was placed in FF inoculating fluid (Biolog™, Hayward, CA, USA) to achieve 75% transmittance equating to approximately 4.44 × 10 4 ascospores mL −1 (Supplementary Table S3 ).…”