The antifungal and antibiofilm activity of Cymbopogon nardus essential oil and citronellal on clinical strains of Candida albicans

Trindade, Leonardo Antunes; Cordeiro, Laísa Vilar; Silva, Daniele de Figuerêdo; Figueiredo, Pedro Thiago Ramalho de; Pontes, Marcela Lins Cavalcanti de; Lima, Edeltrudes de Oliveira; Carvalho, Alessandra de Albuquerque Tavares

doi:10.1007/s42770-022-00740-2

Cited by 11 publications

(4 citation statements)

References 57 publications

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“…The reported studies suggest that citronellal might exert its inhibition effect by affecting the synthesis of ergosterol and biofilm [45,46]. These results are inconsistent with the findings of this study.…”

Section: Discussioncontrasting

confidence: 99%

Natural Product Citronellal can Significantly Disturb Chitin Synthesis and Cell Wall Integrity in Magnaporthe oryzae

Zhou

et al. 2022

JoF

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Background: Natural products are often favored in the study of crop pests and diseases. Previous studies have shown that citronellal has a strong inhibition effect on Magnaporthe oryzae. The objective of this study was to clarify its mechanism of action against M. oryzae. Results: Firstly, the biological activity of citronellal against M. oryzae was determined by direct and indirect methods, and the results show that citronellal had a strong inhibition effect on M. oryzae with EC50 values of 134.00 mg/L and 70.48 μL/L air, respectively. Additionally, a preliminary study on its mechanism of action was studied. After citronellal treatment, electron microscopy revealed that the mycelium became thin and broken; scanning electron microscopy revealed that the mycelium was wrinkled and distorted; and transmission electron microscopy revealed that the mycelium cell wall was invaginated, the mass wall of mycelium was separated, and the organelles were blurred. The mycelium was further stained with CFW, and the nodes were blurred, while the mycelium was almost non-fluorescent after PI staining, and there was no significant difference in the relative conductivity of mycelium. In addition, chitinase was significantly enhanced, and the expression of chitin synthesis-related genes was 17.47-fold upregulated. Finally, we found that the efficacy of citronellal against the rice blast was as high as 82.14% according to indoor efficacy tests. Conclusion: These results indicate that citronellal can affect the synthesis of chitin in M. oryzae and damage its cell wall, thereby inhibiting the growth of mycelium and effectively protecting rice from rice blasts.

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Section: Discussioncontrasting

confidence: 99%

Natural Product Citronellal can Significantly Disturb Chitin Synthesis and Cell Wall Integrity in Magnaporthe oryzae

Zhou

et al. 2022

JoF

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“…Citronellal has been reported to have insecticidal, antifungal, and antimicrobial properties. It is also used as a fragrance in cosmetics and perfumes …”

Section: Resultsmentioning

confidence: 99%

“…It is also used as a fragrance in cosmetics and perfumes. 66 Citral is a monoterpene aldehyde that is a major component of essential oils found in various plants, including C. citratus. Citral is composed of two stereoisomers, namely geranial (cis-3,7-dimethyl-2,6-octadienal) and neral (trans-3,7-dimethyl-2,6octadienal).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Green Synthesis of Zinc Oxide Nanoparticles Using Cymbopogon citratus Extract and Its Antibacterial Activity

Mohammed,

Alghamdi,

Jabbar

et al. 2023

ACS Omega

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Excessive use of antimicrobial medications including antibiotics has led to the emerging menace of antimicrobial resistance, which, as per the World Health Organization (WHO), is among the top ten public health threats facing humanity, globally. This necessitates that innovative technologies be sought that can aid in the elimination of pathogens and hamper the spread of infections. Zinc oxide (ZnO) has multifunctionality owing to its extraordinary physico-chemical properties and functionality in a range of applications. In this research, ZnO nanoparticles (NPs) were synthesized from zinc nitrate hexahydrate, by a green synthesis approach using Cymbopogon citratus extract followed by characterization of the NPs. The obtained X-ray diffraction peaks of ZnO NPs matched with the standard JCPDS card (no. 89-510). The particles had a size of 20–24 nm, a wurtzite structure with a high crystallinity, and hexagonal rod-like shape. UV–Vis spectroscopy revealed absorption peaks between 369 and 374 nm of ZnO NPs synthesized from C. citratus extract confirming the formation of ZnO. Fourier transform infrared confirmed the ZnO NPs as strong absorption bands were observed in the range of 381–403 cm–1 corresponding to Zn–O bond stretching. Negative values of the highest occupied molecular orbital–lowest unoccupied molecular orbital for ZnO NPs indicated the good potential to form a stable ligand–protein complex. Docking results indicated favorable binding interaction between ZnO and DNA gyrase subunit b with a binding energy of −2.93 kcal/mol. ZnO NPs at various concentrations inhibited the growth of Escherichia coli and Staphylococcus aureus. Minimum inhibitory concentration values of ZnO NPs against E. coli and S. aureus were found to be 92.07 ± 0.13 and 88.13 ± 0.35 μg/mL, respectively, at a concentration of 2 mg/mL. AO/EB staining and fluorescence microscopy revealed the ability of ZnO NPs to kill E. coli and S. aureus cells. Through the findings of this study, it has been shown that C. citratus extract can be used in a green synthesis approach to generate ZnO NPs, which can be employed as alternatives to antibiotics and a tool to eliminate drug-resistant microbes in the future.

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“…Our result disagrees with that of a previously published work submitted by Trindade and his colleagues. They suggest that Citronella EO has a strong in vitro antifungal and antibiofilm activity on C. albicans [28].…”

Section: Discussionmentioning

confidence: 99%

Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp

Atron

AHAMADA

Kafa

et al. 2022

Cumhuriyet Science Journal

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Candida species are commonly encountered strains associated with a wide range of infections. Unlike bacterial pathogens, fungal pathogens treatment is difficult and the development of resistance has been increasing at an alarming rate. In this study, the antifungal and antibiofilm effect of thyme oil, rosemary oil, mint oil, citronella oil, was tested on Candida albicans, Candida tropicalis, Candida kefyr, Candida glabrata, Candida parapsilosis isolated from clinical samples. The agar disc diffusion method was employed to determine the antifungal effect of the essential oils, and the inhibition of biofilm formation was assessed using microtiter biofilm inhibition assay. The results indicated that all the essential oils inhibited Candida strains and their biofilm in varying degrees. The highest antifungal activity in all isolates was observed in the thyme oil (>50mm), while rosemary oil showed the highest antibiofilm effect (>77%) in all tested strains. These findings led us to assume that the active components found in essential oils might be potential antifungal agents, adding to the repertoire of therapeutic options for the treatment of candidiasis.

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The antifungal and antibiofilm activity of Cymbopogon nardus essential oil and citronellal on clinical strains of Candida albicans

Cited by 11 publications

References 57 publications

Natural Product Citronellal can Significantly Disturb Chitin Synthesis and Cell Wall Integrity in Magnaporthe oryzae

Natural Product Citronellal can Significantly Disturb Chitin Synthesis and Cell Wall Integrity in Magnaporthe oryzae

Green Synthesis of Zinc Oxide Nanoparticles Using Cymbopogon citratus Extract and Its Antibacterial Activity

Antifungal and Antibiofilm Activities of Some Essential Oils Against Candida spp

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