The antifungal activity of the peptide, periplanetasin-2, derived from American cockroach <i>Periplaneta americana</i>

Yun, Jieun; Hwang, Jae‐Sam; Lee, Dong Gun

doi:10.1042/bcj20170461

Cited by 32 publications

(21 citation statements)

References 63 publications

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“…Because the antimicrobial activity of the hemolymph was induced by abdominal sublethal injection with viable E. coli cells (Basseri et al 2016), innate immunity is probably involved in the response (Kim et al 2016). Although cockroach antimicrobial peptides such as periplanetasin-2 are probably involved in the antimicrobial effect of the hemolymph (Lee et al 2019;Yun et al 2017), the activity is retained in ethanol and ethyl acetate extracts that would denature proteins (Kui et al 2013). This suggests the presence of small antimicrobial molecules which may be of microbial origin (Ali et al 2017).…”

Section: General Outlook For Cockroach-associated Bacteriamentioning

confidence: 99%

Bacteria associated with cockroaches: health risk or biotechnological opportunity?

Guzman

Vilcinskas

2020

Appl Microbiol Biotechnol

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Cockroaches have existed for 300 million years and more than 4600 extant species have been described. Throughout their evolution, cockroaches have been associated with bacteria, and today Blattabacterium species flourish within specialized bacteriocytes, recycling nitrogen from host waste products. Cockroaches can disseminate potentially pathogenic bacteria via feces and other deposits, particularly members of the family Enterobacteriaceae, but also Staphylococcus and Mycobacterium species, and thus, they should be cleared from sites where hygiene is essential, such as hospitals and kitchens. On the other hand, cockroaches also carry bacteria that may produce metabolites or proteins with potential industrial applications. For example, an antibiotic-producing Streptomyces strain was isolated from the gut of the American cockroach Periplaneta americana. Other cockroach-associated bacteria, including but not limited to Bacillus, Enterococcus, and Pseudomonas species, can also produce bioactive metabolites that may be suitable for development as pharmaceuticals or plant protection products. Enzymes that degrade industrially relevant substrates, or that convert biomasses into useful chemical precursors, are also expressed in cockroach-derived bacteria and could be deployed for use in the food/feed, paper, oil, or cosmetics industries. The analysis of cockroach gut microbiomes has revealed a number of lesser-studied bacteria that may form the basis of novel taxonomic groups. Bacteria associated with cockroaches can therefore be dangerous or useful, and this review explores the bacterial clades that may provide opportunities for biotechnological exploitation. Key points • Members of the Enterobacteriaceae are the most frequently cultivated bacteria from cockroaches. • Cultivation-independent studies have revealed a diverse community, led by the phyla Bacteroidetes and Firmicutes. • Although cockroaches may carry pathogenic bacteria, most strains are innocuous and may be useful for biotechnological applications.

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Section: General Outlook For Cockroach-associated Bacteriamentioning

confidence: 99%

Bacteria associated with cockroaches: health risk or biotechnological opportunity?

Guzman

Vilcinskas

2020

Appl Microbiol Biotechnol

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“…This peptide has recently been isolated from the cockroach Periplaneta americana and studied in terms of its mechanism of action against C. albicans [ 113 ]. The synthetic amide version of the peptide led to lipid peroxidation, accumulation of ROS, externalization of phosphatidylserine, dissipation of Δψ , and loss of cytochrome C from mitochondria, with activation of caspases, DNA fragmentation, and eventual cell death.…”

Section: Regulated Cell Death As An Antifungal Peptide Targetmentioning

confidence: 99%

Regulated Cell Death as a Therapeutic Target for Novel Antifungal Peptides and Biologics

Yeaman

Büttner

Thevissen

2018

Oxidative Medicine and Cellular Longevity

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The rise of microbial pathogens refractory to conventional antibiotics represents one of the most urgent and global public health concerns for the 21st century. Emergence of Candida auris isolates and the persistence of invasive mold infections that resist existing treatment and cause severe illness has underscored the threat of drug-resistant fungal infections. To meet these growing challenges, mechanistically novel agents and strategies are needed that surpass the conventional fungistatic or fungicidal drug actions. Host defense peptides have long been misunderstood as indiscriminant membrane detergents. However, evidence gathered over the past decade clearly points to their sophisticated and selective mechanisms of action, including exploiting regulated cell death pathways of their target pathogens. Such peptides perturb transmembrane potential and mitochondrial energetics, inducing phosphatidylserine accessibility and metacaspase activation in fungi. These mechanisms are often multimodal, affording target pathogens fewer resistance options as compared to traditional small molecule drugs. Here, recent advances in the field are examined regarding regulated cell death subroutines as potential therapeutic targets for innovative anti-infective peptides against pathogenic fungi. Furthering knowledge of protective host defense peptide interactions with target pathogens is key to advancing and applying novel prophylactic and therapeutic countermeasures to fungal resistance and pathogenesis.

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“…These changes can be used to determine cell integrity. Of the biochemical and cytological methods used to check pathogen cell integrity after treatment with probiotics include but are not limited to; nuclear fragmentation using DAPI/Tunnel [62][63][64][65][66][67]; in situ ligation assay [65]; DNA laddering [65,66]; externalization of Annexin V/PI by cell membrane [62,64,[67][68][69][70]; mitochondrial and cytosolic calcium [66,67,69,71]; depolarization of the mitochondria using mitochondria membrane potential detection kits for instance, JC fluorescent probes [62,63,[66][67][68][69][70][71]; reactive oxygen species (ROS) accumulation [69] [66, 67, 70, 71]; detecting cytochrome c in cytoplasm using western blotting or color metric kits [63, 66-69, 71, 72]; cytosol / mitochondria intracellular glutathione [67,69]lipid peroxidation [67,69]; potassium release [67] and metacaspace activation detection using kits like CaspACE FITC-VAD-FMK in situ Marker [63,[67][68][69]. The antimicrobial activity of a probiotic microorganism can be assessed using a combination of a number of these methods, which can corroborate the integrity of the indicator pathogen.…”

Section: Ability To Induce Cell Deathmentioning

confidence: 99%

Probiotics and Postbiotics from Food to Health: Antimicrobial Experimental Confirmation

Kosgey¹,

Mwaniki²,

Zhang³

2022

Prebiotics and Probiotics - From Food to Health

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The field of probiotics is up-and-coming, especially in management of microbial pathogens. Probiotics confer nutritional benefits, reduce inflammation and infection. Probiotics have also shown to be helpful in the management of microbial pathogens, which include bacteria, fungi, and viruses. To ernes this potential maximumly, there is a need for an elaborate screening system for new isolates. This entails; rigorous screening methods and thorough confirmatory systems. There is need also to come up with standard methods used to evaluate the probiotics mechanism of action both in vivo and in vitro. In summary, there is a need for a standard screening process for probiotic microorganisms that is reproducible. The aim is to ensure that, the candidate microbial cultures are not written off without proper investigations. This will also fasten the screening process and save time and resources wasted in pre-screening experiments.

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The antifungal activity of the peptide, periplanetasin-2, derived from American cockroach Periplaneta americana

Cited by 32 publications

References 63 publications

Bacteria associated with cockroaches: health risk or biotechnological opportunity?

Bacteria associated with cockroaches: health risk or biotechnological opportunity?

Regulated Cell Death as a Therapeutic Target for Novel Antifungal Peptides and Biologics

Probiotics and Postbiotics from Food to Health: Antimicrobial Experimental Confirmation

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