The antifungal activity of a serine protease and the enzyme production of characteristics of Bacillus licheniformis TG116

Abstract

The growth of Phytophthora capsica, Rhizoctonia solani, Fusarium graminearum, Fusarium oxysporum and Botrytis cinerea were all inhibited by the fermentation supernatant of Bacillus licheniformis TG116, a biocontrol strain isolated from Typhonium giganteum Engl previously with broad-spectrum resistance to plant pathogens. The fermentation supernatant of the TG116 has a great stability on temperature and UV, and shows the biological activity of serine protease and cellulase. The antifungal protease produced by Bacillus licheniformis TG116 was puri ed to homogeneity by ammonium sulfate precipitation, DEAE Sepharose Fast Flow column chromatography and Sephadex G-50 column chromatography. When Colletotrichum capsici was used as an indicator strain, the protease exhibited great antifungal activity at a pH range from 5.0 to 11.0 and temperature at no higher than 60°C. Gene ampli cation veri ed the presence of a gene fragment of serine protease in the strain TG116. The antimicrobial substance isolated from the fermentation broth of TG116 is a serine protease component. The serine protease isolated and puri ed from B. licheniformis TG116 has a great antifungal activity.

Examining the Transcriptomic and Biochemical Signatures of Bacillus subtilis Strains: Impacts on Plant Growth and Abiotic Stress Tolerance

“…Microbial VOCs have been shown to enhance plant tolerance against both abiotic and biotic stress by activating the SA and JA signaling pathways [ 75 ]. Ling et al [ 76 ] demonstrated the antimicrobial activity of microbial serine proteases. Additionally, serine proteases derived from rhizobacteria have been observed to activate plant immune responses and modify cell wall lignification [ 77 ].…”

Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp.

“…Many antagonistic bacteria secrete hydrolytic enzymes such as chitinase, glucanase, and protease, which plays a major role in fungal suppression ( Fatima et al, 2023 ; Xu et al, 2016 ). For example, the proteolytic activity of antagonistic bacteria exhibits strong inhibitory effect on fungal mycelial growth and conidial germination ( Gandhi Pragash et al, 2009 ; Ji et al, 2020 ; Ling et al, 2022 ). In the current study, a qualitative assay of antagonistic substances showed that the B .…”

The Prospect of Hydrolytic Enzymes from Bacillus Species in the Biological Control of Pests and Diseases in Forest and Fruit Tree Production

“…antagonism of phytopathogenic fungi. For instance, the serine proteases (44.3 kDa) from B. licheniformis strain W10 and B. licheniformis strain TG116 demonstrated antifungal activity against B. cinerea [122], and against P. capsica, R. solani, Fusarium sp., and B. cinerea [123], respectively. The protease from B. subtilis strain 21 was effective against F. verticillioides and R. solani in strawberry plants under greenhouse conditions [124], while the protease (31 kDa) from B. licheniformis strain BS-3 was effective against A. niger, M. oryzae, R. solani, and F. oxysporum strains [125].…”