The antifungal action mode of the rice endophyte Streptomyces hygroscopicus OsiSh-2 as a potential biocontrol agent against the rice blast pathogen

Xu, Ting; Cao, Lidan; Zeng, Jiarui; Franco, Christopher M. M.; Yang, Yuanzhu; Hu, Xiaochun; Liu, Ying; Wang, Xiang; Gao, Yan; Bu, Zhigang; Shi, Liming; Zhou, Guoying; Zhou, Qian; Liu, Xuanming; Zhu, Yonghua

doi:10.1016/j.pestbp.2019.06.015

Cited by 59 publications

(39 citation statements)

References 62 publications

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“…In addition, blast disease was reduced by 76.1% in greenhouse experiment at 500 µg/ml of SPM5C-1. Recently, foliar treatment of Streptomyces hygroscopicus OsiSh-2 culture filtrate showed 23.5% and 28.3% disease reduction of P. oryzae in rice seedlings under greenhouse and field trials [23]. In the present study, it was evident that Streptomyces strain PC 12 efficiently reduced blast severity from 87.5% to 31.4% in pathogen-infected plants under greenhouse conditions (Table 3).…”

Section: Discussionsupporting

confidence: 59%

“…Biocontrol bacteria inhibit plant pathogens via several mechanisms, such as the production of antimicrobial compounds (antibiosis), iron sequestration (siderophores), production of extracellular enzymes that interfere with cell wall synthesis (chitinases, cellulase, proteases) and induction of plant-resistance mechanisms [12,16,23]. Rhizosphere Streptomyces in this study showed antagonistic activity against the rice blast fungus, Pyricularia sp., which correlated with their ability to produce siderophores, hydrolytic enzymes and antibiotics (Table 2).…”

Section: Discussionmentioning

confidence: 67%

“…This Streptomyces showed strong in vitro antifungal activity (88.73% inhibition) on the mycelial growth of Magnaporthe oryzae (P. oryzae PTRRC-18). Other studies also indicated the potential of Streptomyces species to control the disease [12,16,[20][21][22][23]. The present study aimed to isolate antagonistic Streptomyces from rice rhizosphere soil and investigate their plant growth promotion and antifungal activity against rice blast fungus Pyricularia sp.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Biocontrol Activities of Streptomyces spp. against Rice Blast Disease Fungi

2020

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Rhizosphere bacteria can positively influence plant growth by direct and indirect mechanisms. A total of 112 bacterial strains were isolated from the rhizosphere of rice and tested for plant beneficial activities such as siderophore production, cell-wall-degrading enzyme production, hydrogen cyanide (HCN) production and antifungal activity against rice blast disease fungus. The actinomycetes count was 3.8 × 106 CFU/g soil. Streptomyces strains PC 12, D 4.1, D 4.3 and W1 showed strong growth inhibition of blast disease fungus, Pyricularia sp. (87.3%, 82.2%, 80.0% and 80.5%) in vitro. Greenhouse experiments revealed that rice plants treated with Streptomyces strain PC 12 recorded maximum plant height, root length and root dry weight compared to the control. Taxonomic characterization of this strain on the basis of 16S rRNA gene sequence led to its identification as Streptomyces palmae PC 12. Streptomyces palmae PC 12 may be used as biofertilizer to enhance the growth and productivity of commercially important rice cultivar RD6 and the biocontrol of blast disease fungus.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Biocontrol Activities of Streptomyces spp. against Rice Blast Disease Fungi

2020

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“…The effective inhibition of mycelial growth or spore production by rapamycin has been observed in several key plant pathogenic fungi, including Botrytis cinerea , 10 Colletotrichum orbiculare , 11 Fusarium graminearum , 12 and Verticillium dahliae 13 . In addition, S. hygroscopicus rapamycin‐producing strains have exhibited biological control effects in field against rice blast, 14 Dendrobium anthracnose disease, 15 and strawberry root rot 16 . However, no studies have investigated the capacity of rapamycin to inhibit B. dothidea and the potential application in the control of cankers on woody plants.…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity of rapamycin on Botryosphaeria dothidea and its effect against Chinese hickory canker

Jiang

Rao

et al. 2020

Pest Management Science

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BACKGROUND: The trunk canker caused by Botryosphaeria dothidea is a devastating disease for Chinese hickory (Carya cathayensis) in China. Chemical fungicides are commonly and repeatedly applied to control the disease; however, fungicide application raises major environmental and food safety issues. Ecofriendly biocontrol alternatives were urgently needed. Herein, the antifungal activity of a natural secondary metabolite, rapamycin, against B. dothidea and the effect of a rapamycin-producing Streptomyces hygroscopicus on C. cathayensis canker were investigated. RESULTS: The 50% effective concentrations (EC 50) of rapamycin against mycelial growth and spore germination on the potato dextrose agar were 5.1 × 10 −3 and 5.5× 10 −2 ∼g mL-1 , respectively, which were much lower than the EC 50 values of thiophanate-methyl. The hyphae of B. dothidea exhibited premature aging and wrinkling after treatment with rapamycin at 5.0 × 10 −3 ∼g mL-1. A rapamycin-producing bacterium S. hygroscopicus LYJ637 was batch produced and formulated in a carboxymethylcellulose/poly (vinyl alcohol) (CMC/PVA) blend and used for testing the efficiency of the bacterium in controlling Botryosphaeria canker in C. cathayensis. S. hygroscopicus exhibited high stability in the CMC/PVA blend. Results of a 3-year field experiment suggested that rapamycin formation reduced the occurrence of both developed cankers and new cankers, with an efficacy comparable to the treatment with thiophanate-methyl. CONCLUSION: The rapamycin-producing S. hygroscopicus LYJ637 carried in a CMC/PVA blend prevented effectively Botryosphaeria canker on Chinese hickory, which provides an alternative approach to chemical control strategies.

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“…Endophytic bacteria can help to resist sheath blight, and the effect of disease resistance is better than that of chemical fungicides. The mechanism of rice endophytes assisting host rice disease resistance mainly includes secretion of antibiotics, hyperparasitism, niche competition and induction of rice self-resistance [27][28] . To resist abiotic stress, endophytic bacteria can help heavy metal contaminated plants to carry out bioremediation, e.g.…”

mentioning

confidence: 99%

Effects of Rare Earth Elements on Bacteria in Rhizosphere, Root, Phyllosphere and Leaf of Soil-Rice Ecosystem

Xin-zhuan

Pan

et al. 2021

Preprint

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The effects of rare earth mining on rice biomass, rare earth element(REE) content and bacterial community structure was studied by pot experiment. The research showed that the REE content in rice roots, shoots and grains was significantly positive correlated with that in soil, and the dry weight of rice roots, shoots and grains was highly correlated with soil physical and chemical properties, nutrient elements and REE contents; The exploitation of rare earth minerals inhibited a-diversity of endophytic fungi in rhizosphere, root, phyllosphere and leaf of rice, significantly reduced the abundance index, OTU number, Chao, Ace index and also significantly reduced the diversity index-Shannon index and Inv-Simpson index, also reduced uniformity index: Pielou’s evenness index, Simpson’s evenness index, etc, which caused β-diversity of bacteria to be quite different. The exploitation of rare earth minerals reduces the diversity of bacteria, but forms dominant bacteria, such as Burkholderia, Bacillus, Buttiauxella, Acinetobacter, Bradyrhizobium, Candida koribacter, which can degrade the pollutants formed by exploitation of rare earth minerals, alleviate the compound pollution of rare earth and ammonia nitrogen, and also has the function of fixing nitrogen and resisting rare earth stress; The content of soil available phosphorus in non mining area is low, forming the dominant bacteria of Pantoea, which has the function of improving soil phosphorus availability. Rare earth elements and physical and chemical properties of soil affect the community structure of bacteria in rhizosphere and phyllosphere of rice, promote the parallel movement of some bacteria in rhizosphere, root, phyllosphere and leaf of rice, promote the construction of community structure of bacteria in rhizosphere and phyllosphere of rice, give full play to the growth promoting function of Endophytes, and promote the growth of rice. The results showed that the rice biomass in mining area was guaranteed due to the growth promoting effect of endophytic bacteria, However, the mining of mineral resources causes the compound pollution of rare earth and ammonia nitrogen, which makes REE content of rice in mining area significantly higher than that in non mining area.

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The antifungal action mode of the rice endophyte Streptomyces hygroscopicus OsiSh-2 as a potential biocontrol agent against the rice blast pathogen

Cited by 59 publications

References 62 publications

Evaluation of Biocontrol Activities of Streptomyces spp. against Rice Blast Disease Fungi

Evaluation of Biocontrol Activities of Streptomyces spp. against Rice Blast Disease Fungi

Antifungal activity of rapamycin on Botryosphaeria dothidea and its effect against Chinese hickory canker

Effects of Rare Earth Elements on Bacteria in Rhizosphere, Root, Phyllosphere and Leaf of Soil-Rice Ecosystem

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