The Antidiabetic Activity of an Aqueous Root Extract of<i>Annona stenophylla</i>Engl. and Diels in Non-diabetic Control and Alloxan-induced Diabetic rats

Taderera, Tafadzwa; Gomo, Exnevia; Chagonda, Lameck S.

doi:10.1080/22311866.2016.1234412

Cited by 8 publications

(6 citation statements)

References 15 publications

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“…In previous studies the aqueous extract of Annona stenophylla demonstrated hypoglycaemic and insulinotropic effects in alloxan-induced diabetic rats18. Western diabetic drugs correct hypoglycemia by supplementing insulin, improving insulin sensitivity, increasing insulin secretion from the pancreas and/or glucose uptake by tissue cells27.…”

Section: Discussionmentioning

confidence: 96%

“…Earlier in our laboratory we have demonstrated that A. stenophylla plant extract possess antioxidant activity, antimicrobial activity and has potential hypoglycaemic effects. The hypoglycaemic activity has partly been attributed to its ability to inhibit carbohydrate metabolising enzymes and a polyherbal formulation encoperating A. stenophylla has been formulated15,16,17,18,19. The extract has been found to be relatively safe to use through brineshrimp lethality tests and subacute toxicity tests using a rat model20,21.…”

Section: Introductionmentioning

confidence: 99%

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Annona stenophylla aqueous extract stimulate glucose uptake in established C2Cl2 muscle cell lines

Taderera¹,

Chagonda²,

Gomo³

et al. 2019

Afr H. Sci.

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BackgroundAnnona stenophylla is a folk medicine popularly used in Zimbabwe for the treatment of many ailments. This study was carried out to determine some of the possible anti diabetic mechanisms of its action using in vitro cell culturing methods.MethodsA. stenophylla's effects on glucose uptake were tested using muscle cells (C2Cl2). Expression of glucose 4 transporters was determined by treating cell lines with plant extract. Total RNA was isolated and using RT-PCR, GLUT 4 expression levels were quantified. Translocation of GLUT 4 was assessed using FITC fluorescence measured by flow cytometry.ResultsTreatment of cells with plant extract significantly increased glucose uptake in a concentration dependent manner, with the highest concentration (250 µg/ml) giving 28% increased uptake compared to the negative control. The increase in glucose uptake (2.5 times more than control) was coupled to increase in GLUT 4 mRNA and subsequently GLUT 4 translocation. Wortmannin expunged the A. stenophylla induced increase in GLUT 4 mRNA and glucose uptake.ConclusionThe results suggest that A. stenophylla aqueous extract increases glucose uptake partly through increasing the GLUT 4 mRNA and translocation potentially acting via the PI-3-K pathway. This study confirms the ethnopharmacological uses of A. stenophylla indicating potential for anti-diabetic products formulation.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Annona stenophylla aqueous extract stimulate glucose uptake in established C2Cl2 muscle cell lines

Taderera¹,

Chagonda²,

Gomo³

et al. 2019

Afr H. Sci.

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“…& Diels Colds, bronchitis and pulmonary troubles Antimycobacterial, antimicrobial and antimalarial activities. Highly toxic LC 50 values ranging from 0.56 to 66.07 μg/mL Malebo et al (2014) ; Dzoyem et al (2016) ; Chauke et al (2016) ; Taderera et al (2016) ; Souham et al (2018) Apiaceae Alepidea amatymbica Eckl. & Zeyh Asthma, influenza, colds and cough Antimicrobial, anti-inflammatory and antiviral activities.…”

Section: Methodsmentioning

confidence: 99%

Medicinal plants used for the management of respiratory diseases in Zimbabwe: Review and perspectives potential management of COVID-19

Nyagumbo¹,

Pote²,

Shopo³

et al. 2022

Physics and Chemistry of the Earth, Parts A/B/C

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“…A 6-hrs. quick is considered to be the perfect time for rats to develop an oral glucose tolerance test (OGTT) 30,31 . The rise in blood glucose in each group reported the successful loading of oral glucose after 30 min.…”

Section: Antidiabetic Effect Of Ceps Extract In Alloxan Diabetic Ratsmentioning

confidence: 99%

Antidiabetic Activity, Alpha-Amylase and Alpha- Glucosidase Inhibitory Effect of Pastinaca Sativa Extract

Nagaraju

Rajasekhar

et al. 2021

Bulletin of Pharmaceutical Sciences. Assiut

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Pastinaca sativa belonging to the family Apiaceae, is a tropical tree used in many countries as a herbal drug for the care of diabetic patients. However, the methodical rationale for this medical use is very limited. The aim of this analysis was to examine the antidiabetic activity of the Pastinaca sativa methanolic extract and the possible mechanisms underlying that activity. The extract hypoglycemic behavior was investigated in typical diabetic rats induced with alloxan. Finally, an effect of the Pastinaca sativa extract (Crude extract of Pastinaca sativa-CEPS) on the activity of α-amylase and α-glucosidase was examined in vitro. CEPS showed IC50 equal to 91.69 ± 1.5 μg/mL for α-amylase enzyme inhibition activity. Normal acarbose (control) demonstrated IC50 equal to 83.25 ± 1.28 μg/mL. CEPS displayed IC50 values of 88.05 ± 1.25 μg/mL for α-glucosidase enzyme. Under similar laboratory conditions, acarbose displayed an IC50 value of 51.00 ± 1.23 μg/mL. CEPS exhibited IC50 less than 100μg/mL would be considered as healthy. The extract at 400 mg/kg greatly decreased the region under the blood glucose level curve in a typical rats test for oral glucose tolerance. A single dose of the extract decreased significantly in the alloxan-induced diabetic model, similar to glibenclamide (standard), from 208.33 mg/dl to 106.38 mg/dl at 200 mg/kg CEPS and from 209.82 mg/dl to 111.65 mg/dl at 400 mg/kg CEPS. These findings were comparable with 0.5 mg/kg of glibenclamide indicating a substantial decrease from 205.55 mg/dl on 7th day to 84.88 mg/dl. The Pastinaca sativa methanolic extract possesses strong antidiabetic activity in vivo. Besides, the extract has also been shown to have a significant inhibitory activity of αamylase and α-glucosidase, which might lead to its anti-hyperglycemic function when used in diabetic patients.

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The Antidiabetic Activity of an Aqueous Root Extract ofAnnona stenophyllaEngl. and Diels in Non-diabetic Control and Alloxan-induced Diabetic rats

Cited by 8 publications

References 15 publications

Annona stenophylla aqueous extract stimulate glucose uptake in established C2Cl2 muscle cell lines

Annona stenophylla aqueous extract stimulate glucose uptake in established C2Cl2 muscle cell lines

Medicinal plants used for the management of respiratory diseases in Zimbabwe: Review and perspectives potential management of COVID-19

Antidiabetic Activity, Alpha-Amylase and Alpha- Glucosidase Inhibitory Effect of Pastinaca Sativa Extract

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