The anticancer effects of actinoporin RTX-A from the sea anemone Heteractis crispa (=Radianthus macrodactylus)

Fedorov, Sergey N.; Dyshlovoy, Sergey A.; Monastyrnaya, Margarita; Shubina, Larisa K.; Leychenko, Elena; Kozlovskaya, É. P.; Jin, Jun‐O; Kwak, Jong Young; Bode, Ann M.; Dong, Zigang; Stonik, Valentin A.

doi:10.1016/j.toxicon.2009.11.016

Cited by 58 publications

(44 citation statements)

References 33 publications

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“…and C. sericeum showed both hemolytic and cytotoxic activities, and thus might have pore-forming cytolytic toxins such as actinoporins. An anticancer effect in a member of the actinoporins has been reported [31]. Thus, the hemolytic compounds from deep-sea jellyfish might have the potential for such a medicinal use.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the bioactivities of water-soluble extracts from twelve deep-sea jellyfish species

et al. 2013

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Many polypeptides isolated from shallow water cnidarian species have been utilized as valuable biochemical tools in both basic and applied biological sciences. Deepwater cnidarian species might be another potential resource for novel biochemical tools. However, because of limited access to cnidarian samples from deep-sea environments, bioactive polypeptides have never before been reported from this group. In this study, we collected twelve deep-sea jellyfish species (nine hydrozoans and three scyphozoans) using a plankton net that was specially designed for collecting deep-sea organisms, and prepared water-soluble extracts, presumably containing polypeptides, of these jellyfishes. The extracts were subjected to cytotoxicity, hemolytic activity, and crustacean lethal toxicity tests. In the cytotoxicity test, six out of the nine tested hydrozoan species showed activity. In the hemolytic activity test, only three hydrozoans showed activity and none of the scyphozoan jellyfishes showed activity. In the crustacean lethality test, two hydrozoan jellyfishes and all three of the tested scyphozoan jellyfishes showed lethal activity. These results revealed a high incidence of watersoluble bioactive substances occurring in these deepsea jellyfishes. Furthermore, all the heat-treated and the methanol-treated crude jellyfish extracts lost their bioactivities. Thus, it is likely that the bioactive compounds in the water-soluble extracts were unstable polypeptides (proteins). This is the first published report on bioactivities in extracts from deep-sea jellyfishes.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the bioactivities of water-soluble extracts from twelve deep-sea jellyfish species

et al. 2013

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Section: Discussionmentioning

confidence: 99%

“…CI41 cells (Fedorov et al 2010). RTX-A toxin (H. crispa), induces P53-independent apoptosis and inhibits the activation of the oncogenic nuclear transcriptional factors AP-1 and NF-KB (Fedorov et al 2010). The intrinsic and extrinsic apoptosis pathways converge with the activation of caspase-3 and subsequently other executioner caspases and nucleases drive the terminal events of programmed cell death (MacFarlane 2003;Crow et al 2004;Prunell et al 2005;Elmore 2007;Pizon et al 2011).…”

Section: Discussionmentioning

confidence: 99%

The effect of sea anemone (H. magnifica) venom on two human breast cancer lines: death by apoptosis

2013

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Venom from the sea anemone, Heteractis magnifica, has multiple biological effects including, cytotoxic, cytolytic and hemolytic activities. In this study, cytotoxicity induced by H. magnifica venom was investigated using the crystal violet assay on human breast cancer T47D and MCF7 cell lines and normal human breast 184B5 cell line. Apoptosis was also assayed via Annexin V-flourescein isothiocyanate and propidium iodide (PI) staining followed by flow cytometric analysis. Cell cycle progression and mitochondria membrane potential were studied via flow cytometry following PI and JC-1 staining respectively. H. magnifica venom induced significant reductions in viable cell numbers and increases in apoptosis in T47D and MCF7 in dose-dependent manners. A significant apoptosisrelated increase in the sub G1 peak of the cell cycle in both breast cancer cell lines was also observed. Moreover, treatment by venom cleaved caspase-8, caspase-9, and activated caspase-3. Overall, H. magnifica venom was highly cytotoxic to T47D and MCF7 human breast cancer cells, and the phenomenon could be the killing phenomenon via the death receptor-mediated and the mitochondria-mediated apoptotic pathways. Consequently, H. magnifica venom has potential for the development of a breast cancer therapeutic.

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“…Furthermore, studies found that sea nettle nematocyst venom (SNV) peptide possessed significant antitumor effect on Ehrlich ascites carcinoma (EAC) tumor (Balamurugan et al, 2010). Four isoforms of actinoporin (RTX-A), isolated from the tropical sea anemone Heteractis crispa (=Radianthus macrodactylus), demonstrated potential cancer-preventive activity at extremely low and non-cytotoxic concentrations through the induction of p53-independent apoptosis and inhibition of the oncogenic AP-1 and NF-κB nuclear factors activity (Fedorov et al, 2010).…”

Section: Antitumor Activity Of Other Animal Toxinsmentioning

confidence: 99%

Biotoxins for Cancer Therapy

Liu

Yang²,

Zhang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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In recent times, a number of studies have provided evidence that biotoxins present great potential as antitumor agents, such as snake venom, bee venom, some bacteria toxins and plant toxins, and thus could be used as chemotherapeutic agents against tumors. The biodiversity of venoms and toxins make them a unique source from which novel anticancer agent may be developed. Biotoxins, also known as natural toxins, include toxic substances produced by plants, animals and microorganisms. Here, we systematically list representative biological toxins that have antitumor properties, involving animal toxins, plant toxins, mycotoxins as well as bacterial toxins. In this review, we summarize the current knowledge involving biotoxins and the active compounds that have anti-cancer activity to induce cytotoxic, antitumor, immunomodulatory, and apoptotic effects in different tumor cells in vivo or in vitro. We also show insights into the molecular and functional evolution of biotoxins.

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The anticancer effects of actinoporin RTX-A from the sea anemone Heteractis crispa (=Radianthus macrodactylus)

Cited by 58 publications

References 33 publications

Evaluation of the bioactivities of water-soluble extracts from twelve deep-sea jellyfish species

Evaluation of the bioactivities of water-soluble extracts from twelve deep-sea jellyfish species

The effect of sea anemone (H. magnifica) venom on two human breast cancer lines: death by apoptosis

Biotoxins for Cancer Therapy

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