The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-α1 and Erk1/2 Signalling

Abstract

In the present study, we aimed to evaluate the anticancer activities of Cetraria islandica (C.islandica) extracts on MCF-7 breast cancer cell lines. Cell viability, protein levels, apoptotic cells number, F-actin distribution were measured. Cell viability of MCF-7 breast cancer cells was found to be reduced in a dose-dependent manner.EC50 values of C.islandica on MCF-7 cells were found to be 9.2047 E-5 g/ml (cell amount) by using intelligence system. Expressions of p53, caspase 3 and Bcl-2, were shown to be elevated after low doses of extract and diminished after high dose treatments. PPAR- protein level was decreased, although AMP-activated kinases-α1 (AMPK-α1) protein level was increasedin its extract groups. ERK1/2 protein level was also elevated in its extract groups. 125 mg/ml of extract treated cells show a low decrease in actin filament density. MCF-7 cells with C.islandica treatment for 24 h increased the apoptotic cell percentage, though the cells-treated with C.islandica for 48 was high necrotic cells percentage. Consequently, the C.islandica extract treatment causes to elevate ERK1/2 and AMPK-α1 protein levels, resulting in PPAR- and then triggers the apoptosis by modulation caspase-3 and P53 protein levels. Therefore, C.islandica might be a good candidate for anticancer tissue, especially soft tissue tumours.

The Genus Cetraria s. str.—A Review of Its Botany, Phytochemistry, Traditional Uses and Pharmacology

“…Hence, the methanol extract of Cetraria islandica reduced the cell viability of the human breast cancer cells (MCF-7) (IC 50 value of 19.51 µg/mL), the human liver cancer cells (HepG2) (IC 50 value of 181.05 µg/mL), human melanoma cells (FemX) (IC 50 value of 22.68 μg/mL) and human colon carcinoma cells (LS174) (IC 50 value of 33.74 μg/mL) [ 15 , 121 ]. On the other hand, the ethanol extract of Cetraria islandica was able to reduce cell viability in MCF-7 cells (IC 50 9.2047 × 10 −5 g/mL), also showing an increase in protein levels of AMP-activated kinases-α1 (AMPK-α1) and ERK1/2 [ 124 ]. The compound protolichesterinic acid, isolated from Cetraria islandica , decreased cell viability and caused morphological changes at a concentration of 20 µg/mL on breast carcinomas T-47D and ZR-75-1 and erythro-leukaemia K-562 cells.…”