The antibody response of cattle infected with bovine immunodeficiency virus to peptides of the viral transmembrane protein.

Scobie, Linda; Venables, C.; Hughes, Kenneth; Dawson, M.; Jarrett, Oswald

doi:10.1099/0022-1317-80-1-237

Cited by 17 publications

(16 citation statements)

References 23 publications

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“…The samples collected for this study formed a final bank containing 1,168 plasma and PBL/DNA samples from animals in 17 different production farms distributed across 10 Mexican states (Table ). Like other studies (Albernaz et al, ; Meas, Ohashi, Sugimoto, & Onuma, ; Meas, Ohashi, et al, ; Meas et al, ; Scobie, Venables, Hughes, Dawson, & Jarrett, ; St.Cyr Coats, Pruett, Nash, & Cooper, ; Suarez et al, ), we used bioinformatic and synthetic resources, as well as serological and molecular procedures that allowed us to confirm a cattle infection so far unknown in Mexico.…”

Section: Resultsmentioning

confidence: 99%

“…Several protocols focused on serological detection have been developed during studies of BIV presence in diverse regions. These have evaluated the use of purified antigens from cell cultures (Burkala, Ellis, Voigt, & Wilcox, ), of recombinant antigens (Abed & Archambault, ; Burkala et al, ; Polack et al, ; Zheng et al, ) and synthetic antigens (Scobie et al, ). In this study, we used an iELISA protocol with a synthetic peptide designed via an in silico analysis as an antigen.…”

Section: Resultsmentioning

confidence: 99%

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First evidence of bovine immunodeficiency virus infection in Mexican cattle

González-Fernández

Pérez

Flores

et al. 2020

Transbound Emerg Dis

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This study set out to identify the presence of bovine immunodeficiency virus (BIV) in animals geographically located in Mexico. BIV was first discovered in the United States in a dairy cow with persistent lymphocytosis, lymphoid hyperplasia and lymphocytic encephalitis. Many studies indicate that BIV infection is globally distributed, but its presence in Mexico remains unknown. We collected 1,168 heparinized blood samples from cattle in ten states across the Mexican Republic, then separated plasma using centrifugation and tested for antibodies against BIV. We used an indirect ELISA based on the use of a synthetic peptide derived from transmembrane glycoprotein (gp45/TM). In order to identify the viral genome, we designed a synthetic gene as a PCR control, as well as a pair of oligonucleotides for amplifying a 519 bp product of the env gene which encodes the surface protein. Positive amplicons were purified and subjected to nucleotide sequencing. A total of 189 (28.94%) tested plasma samples suggest the presence of specific anti‐BIV antibodies in all states studied except for Chiapas. Additionally, PCR results identified six positive cows in the states of Puebla and Coahuila. BIV in these cows was confirmed via nucleotide sequencing and in silico analysis of these samples. This is the first report of the presence of BIV in Mexican cattle.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

First evidence of bovine immunodeficiency virus infection in Mexican cattle

González-Fernández

Pérez

Flores

et al. 2020

Transbound Emerg Dis

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“…The estimated sensitivity was found to be 100%, since all 23 sera previously defined as positive by western immunoblot were positive. Since these sera were also positive by TM-ELISA derived from the FL112 isolate (Scobie et al 1999), the conclusion can therefore be made that our ELISA is ideal for BIV serodiagnosis. In this study, the ELISA achieved 94% of specificity and this was slightly higher than obtained by Zheng et al (2000).…”

Section: Discussionmentioning

confidence: 99%

Serological survey for bovine immunodeficiency virus in dairy cattle from Poland

Rola–Łuszczak¹,

Kozaczynska²,

Kuźmak³

2011

Polish Journal of Veterinary Sciences

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A seroprevalence study of bovine immunodeficiency virus (BIV) was undertaken on 1,541 serum samples from Holstein cattle from 23 herds, located in different geographical regions of Poland. The analysis was performed using ELISA, with recombinant Gag protein of BIV as antigen. The average BIV prevalence was 4.9% in individual cattle, while the percentage of herds harboring at least one seropositive animal, was 82.6%. To demonstrate the correlation of BIV and bovine leukemia virus infection, all sera were analysed for BLV antibodies and there was only a slight association between both infections. Overall, these results show that BIV infection is present in dairy cattle in Poland at a prevalence rate found in other European countries.

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“…Der Nachweis von Antikörpern im Serum der untersuchten Tiere erfolgte mittels des von Scobie et al (13) beschriebenen indirekten ELISA (enzyme-linked immunosorbent assay), der in einigen Arbeitsschritten modifiziert wurde. In diesem ELISA wird ein zyklisches Transmembranprotein des BIV als Antigen verwendet (14).…”

Section: Nachweis Der Antikörper Mittels Elisaunclassified

Vorkommen von Antikörpern gegen das bovine Immunschwächevirus bei Rindern in Bayern

Bartels¹,

Hartmann²,

Scobie³

et al. 2003

Tierarztl Prax Ausg G

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ZusammenfassungIm Rahmen einer epidemiologischen Untersuchung zur Infektion mit dem bovinen Immunschwächevirus (BIV) bei Rindern in Oberbayern erfolgten zwei Studien, in denen Serum mittels indirektem ELISA auf BIV-Antikörper untersucht wurde. Die ELISA-Ergebnisse der BIV-positiven Tiere der Studie I wurden mittels Western Blot bestätigt. In Studie I wurde Blut von 173 ungezielt ausgewählten Rinderpatienten der II. Medizinischen Tierklinik der Ludwig-Maximilians-Universität München untersucht. Von diesen waren acht Tiere BIV-infiziert. Das entspricht einer Prävalenz von 4,6%. Alle positiven Tiere waren über zwei Monate alt. In Studie II wurden 550 Kühe aus 11 oberbayerischen landwirtschaftlichen Betrieben untersucht. Hiervon waren 11 Tiere BIVAntikör-perpositiv. Dies entspricht einer Prävalenz von 2,0%. Die positiven Tiere stammten aus fünf Betrieben mit Boxenlaufstallhaltung. Kein Tier aus Betrieben mit Anbindehaltung war positiv. In Studie II lag das Durchschnittsalter der Kühe aus den Betrieben ohne BIV-infizierte Tiere signifikant höher als in den Betrieben mit BIV-infizierten Tieren. Die Prävalenz von BIV-Antikörpern war zwar unter den kranken Probanden aus Studie I signifikant höher als bei den klinisch unauffälligen Rindern der Studie II, die pathogene Bedeutung des BIV erscheint jedoch fraglich.

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The antibody response of cattle infected with bovine immunodeficiency virus to peptides of the viral transmembrane protein.

Cited by 17 publications

References 23 publications

First evidence of bovine immunodeficiency virus infection in Mexican cattle

First evidence of bovine immunodeficiency virus infection in Mexican cattle

Serological survey for bovine immunodeficiency virus in dairy cattle from Poland

Vorkommen von Antikörpern gegen das bovine Immunschwächevirus bei Rindern in Bayern

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