The Antibacterial Type VII Secretion System of Bacillus subtilis: Structure and Interactions of the Pseudokinase YukC/EssB

Tassinari, Matteo; Doan, Thierry; Bellinzoni, M.; Chabalier, Maïalène; Ben-Assaya, Mathilde; Martínez, Mariano; Gaday, Quentin; Alzari, Pedro M.; Cascales, Éric; Fronzes, Rémi; Gubellini, Francesca

doi:10.1128/mbio.00134-22

Cited by 26 publications

(34 citation statements)

References 106 publications

(148 reference statements)

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“…EssB, which also has a single transmembrane helix, has an N-terminal cytoplasmic pseudokinase-like domain and a C-terminal extracellular domain through which the protein dimerises. The extracellular domain is hypothesised to interact with other components, substrates or possibly the cell wall [43,49]. The pseudokinase domain of EssB, while lacking catalytic residues, retains structural similarity to the substrate-binding region of protein kinases [49].…”

Section: T7ssb-specific Core Components: Esaa Essa and Essbmentioning

confidence: 99%

“…EsaA, EssB EsaA interaction with EssB transmembrane domain, by BACTH. [43,120] EssA, EssB EssA -EssB direct interaction by BACTH. [43] EssA, EsaB EsaB -EssA direct interaction by BACTH.…”

Section: The T7ssb As a Bacterial Weapon Variable Contribution Of The...mentioning

confidence: 99%

“…Preliminary studies with the B. subtilis EsaB homologue YukD support the idea that EsaB might, like EccD, interact with other transmembrane membrane components, specifically EssA and/or EssB [43].…”

Section: Components and Assembly Of The T7ssbmentioning

confidence: 99%

“…aureus strain USA300 with interactions detected between all six core components [42, 50], and many of these interactions have been similarly detected in B. subtilis [43]. However, an alternative study failed to detect interactions between membrane components in S.…”

Section: Components and Assembly Of The T7ssbmentioning

confidence: 99%

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Interbacterial competition mediated by the type VIIb secretion system

Boardman,

Palmer,

Alcock

2023

Microbiology

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Successful occupancy of a given niche requires the colonising bacteria to interact extensively with the biotic and abiotic environment, including other resident microbes. Bacteria have evolved a range of protein secretion machines for this purpose with eleven such systems identified to date. The type VIIb secretion system (T7SSb) is utilised by Bacillota to secrete a range of protein substrates, including antibacterial toxins targeting closely related strains, and the system as a whole has been implicated in a range of activities such as iron acquisition, intercellular signalling, host colonisation and virulence. This review covers the components and secretion mechanism of the T7SSb, the substrates of these systems and their roles in Gram-positive bacteria, with a focus on interbacterial competition.

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Section: T7ssb-specific Core Components: Esaa Essa and Essbmentioning

confidence: 99%

“…EsaA, EssB EsaA interaction with EssB transmembrane domain, by BACTH. [43,120] EssA, EssB EssA -EssB direct interaction by BACTH. [43] EssA, EsaB EsaB -EssA direct interaction by BACTH.…”

Section: The T7ssb As a Bacterial Weapon Variable Contribution Of The...mentioning

confidence: 99%

Section: Components and Assembly Of The T7ssbmentioning

confidence: 99%

Section: Components and Assembly Of The T7ssbmentioning

confidence: 99%

See 2 more Smart Citations

Interbacterial competition mediated by the type VIIb secretion system

Boardman,

Palmer,

Alcock

2023

Microbiology

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“…Bacillus subtilis) species (7,8). The primary role of the T7SSb is inhibition of competitor bacteria via secretion of antibacterial toxins (9)(10)(11)(12)(13)(14)(15). These toxin substrates usually have a transport-associated N-terminal LXG domain (IPR006829), and a highly polymorphic Cterminal toxin domain.…”

Section: Introductionmentioning

confidence: 99%

Three small partner proteins facilitate the type VII-dependent secretion export of an antibacterial nuclease

Palmer

Yang

Boardman

et al. 2023

Preprint

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The type VIIb secretion system (T7SSb) is a multisubunit protein export machine found in Gram-positive Firmicute bacteria which plays a key role in interbacterial competition. The T7SSb secretes a variety of toxic effector proteins targeting closely related strains, however the mechanism of secretion and the roles of numerous conserved genes within T7SSb gene clusters remain unknown. EsaD is a nuclease toxin secreted by the Staphylococcus aureus T7SSb, which forms a pre-secretion complex with its cognate immunity protein, EsaG, and chaperone EsaE. Encoded upstream of EsaD are three small secreted proteins of unknown function, EsxB, EsxC and EsxD. Here we show that these three proteins bind to EsaD and function as EsaD export factors and we report the first structural information for a complete T7SSb substrate pre-secretion complex. Cryo-EM of the EsaDEG trimer and the EsaDEG-EsxBCD hexamer shows that incorporation of EsxBCD confers an elongated conformation comprising a flexible globular cargo domain attached to a long narrow shaft that is likely to be crucial for efficient toxin export.

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Assembly of an active microbial consortium by engineering compatible combinations containing foreign and native biocontrol bacteria of kiwifruit

Lin

Tao

et al. 2023

Computational and Structural Biotechnology Journal

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The Antibacterial Type VII Secretion System of Bacillus subtilis: Structure and Interactions of the Pseudokinase YukC/EssB

Cited by 26 publications

References 106 publications

Interbacterial competition mediated by the type VIIb secretion system

Interbacterial competition mediated by the type VIIb secretion system

Three small partner proteins facilitate the type VII-dependent secretion export of an antibacterial nuclease

Assembly of an active microbial consortium by engineering compatible combinations containing foreign and native biocontrol bacteria of kiwifruit

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