The antibacterial peptide ABP-CM4: the current state of its production and applications

Li, Jian Feng; Zhang, Jie; Xu, Xing; Han, Yang; Cui, Xian Wei; Chen, Yu Qing; Zhang, S. Q.

doi:10.1007/s00726-011-0982-3

Cited by 16 publications

(12 citation statements)

References 56 publications

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“…In opposition to antibiotics, which act in specific intracellular targets, AMPs mostly interact with the negatively charged bacterial cell membranes through electrostatic forces, inducing physical changes and damaging the biological membranes [26][27][28]51]. Although not being clearly understood, the antimicrobial action of ABP-CM4 is generally accepted to be a consequence of an interaction with the cell membrane, leading to the formation of transmembrane pores that result in cell death [52]. This action at the cell is in agreement with our results suggesting that the antimicrobial activity of the fibres is a timedependent event that implies a direct contact with the bacterial cells.…”

Section: Discussionmentioning

confidence: 99%

Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer

et al. 2017

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Genetically engineered protein polymers functionalized with bioactive domains offer potential as multifunctional versatile materials for biomedical use. The present work describes the fabrication and characterization of antimicrobial fibre mats comprising the antimicrobial elastin-like recombinamer CM4-A200. The CM4-A200 protein polymer derives from the genetic fusion of the ABP-CM4 antimicrobial peptide from Bombyx mori with 200 repetitions of the pentamer VPAVG. This is the first report on non-crosslinked fibre mats fabricated with an antimicrobial elastin-like recombinamer stable in solution.Thermal gravimetric analysis of CM4-A200 fibre mats shows one single degradation step at temperatures above 300 ºC, with fibres displaying a higher thermal degradation activation. The electrospun CM4-A200 fibres display high antimicrobial activity against Gram-positive and Gram-negative bacteria with no detectable cytotoxic effects against normal human skin fibroblasts and keratinocytes, revealing the great potential of these polymers for the fabrication of biomedical materials.

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Section: Discussionmentioning

confidence: 99%

Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer

et al. 2017

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“…Gel‐migration experiment shows that B. mori Cecropin A (BmCecA, NP_001037462) may act on genomic DNA and RNA after destroying bacterial membranes, which leads to rapid death of the bacteria (Lv et al, ). The antibacterial peptide CM4 (ABP‐CM4) from the silkworm exhibits strong activity against fungi, such as Aspergillus niger and Trichoderma viride , as well as against gibberellic disease (Zhang et al, ; Li et al, ). The novel AMP Cecropin‐XJ (NM_001102561), isolated and purified from the silkworm larvae, exhibits high thermal stability, high pH adaptability and broad antibacterial activity.…”

Section: Ampsmentioning

confidence: 99%

Research progress on the immune mechanism of the silkworm Bombyx mori

et al. 2018

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The silkworm Bombyx mori L., representing an important economic insect and one of the best models for studying insect immunity, possesses an efficient and sophisticated innate immune system against invasive microorganisms. The innate immune system basically includes humoural immunity and cellular immunity. The humoural immunity, which functions via molecules including humoural factors, lysozymes, phenoloxidase, hemolin, lectins and, in particular, antimicrobial peptides, plays a central role in eliminating the invading pathogens. The cellular immunity is primarily carried out and mediated by plasmatocytes and granular cells of haemocytes in the haemolymph, usually followed by melanization. Additionally, apoptosis, a primary viral defence for insects lacking adaptive immunity, comprises an important part of the silkworm immune system. Currently, there is still the lack of a comprehensive and systematic understanding of the molecular mechanisms of silkworm immunity. We review the latest research progress on silkworm immune mechanisms, including phenoloxidase‐dependent melanization and apoptosis, which is conducive to improving our understanding of the silkworm immune mechanism, clarifying the relationship of various immune mechanisms, and also providing a theoretical basis and reference for the future research of insect immunity.

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“…Cationic amphiphilic peptide CM4 is an effective AMP against bacteria as well as fungi that functions by affecting membrane interactions ( Zhang et al, 2008 ; Li et al, 2012 ). It also has anti-inflammatory effects mediated by the neutralization of the endotoxin lipopolysaccharide and anticancer activity in leukemia cells by targeting and disrupting the plasma membrane ( Lin et al, 2008 ; Chen et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

N-myristoylation of Antimicrobial Peptide CM4 Enhances Its Anticancer Activity by Interacting With Cell Membrane and Targeting Mitochondria in Breast Cancer Cells

Liu

Yang

et al. 2018

Front. Pharmacol.

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Development of antimicrobial peptides (AMPs) as highly effective and selective anticancer agents would represent great progress in cancer treatment. Here we show that myristoyl-CM4, a new synthetic analog generated by N-myristoylation of AMPs CM4, had anticancer activity against MCF-7, MDA-MB-231, MX-1 breast cancer cells (IC50 of 3–6 μM) and MDA-MB-231 xenograft tumors. The improved activity was attributed to the effect of myristoyl on the cell membrane. Flow cytometry and confocal laser scanning microscopy results showed that N-myristoylation significantly increased the membrane affinity toward breast cancer cells and also effectively mediated cellular entry. Despite increasing cytotoxicity against HEK293 and NIH3T3 cells and erythrocytes associated with its anticancer activity, myristoyl-CM4 maintained a certain selectivity toward breast cancer cells. Accordingly, the membrane affinity toward breast cancer cells was two to threefold higher than that of normal cells. Glycosylation analysis showed that sialic acid-containing oligosaccharides (including O-mucin and gangliosides) were important targets for myristoyl-CM4 binding to breast cancer cells. After internalization, co-localization analysis revealed that myristoyl-CM4 targeted mitochondria and induced mitochondrial dysfunction, including alterations in mitochondrial transmembrane potential, reactive oxygen species (ROS) generation and cytochrome c release. Activation of caspase 9, caspase 3 and cleavage of PARP were observed in MX-1, MCF-7, and MDA-MB-231 cells after myristoyl-CM4 treatment. The current work indicates that increasing hydrophobicity by myristoylation to modulate peptide-membrane interactions and then target mitochondria is a good strategy to develop AMPs as anticancer agents in the future.

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The antibacterial peptide ABP-CM4: the current state of its production and applications

Cited by 16 publications

References 56 publications

Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer

Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer

Research progress on the immune mechanism of the silkworm Bombyx mori

N-myristoylation of Antimicrobial Peptide CM4 Enhances Its Anticancer Activity by Interacting With Cell Membrane and Targeting Mitochondria in Breast Cancer Cells

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