The antibacterial effects of coffee extract, chlorhexidine, and fluoride against Streptococcus mutans and Lactobacillus plantarum: An in vitro study

Akhlaghi, Najmeh; Sadeghi, Marziye; Fazeli, Fataneh; Akhlaghi, Shiva; Mehnati, Maryam; Sadeghi, Masoud

doi:10.4103/1735-3327.266093

Cited by 16 publications

(21 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…None of the standard fluoride and nystatin mouthwashes investigated in the study prevented the growth of S. mutans. That was not unexpected for nystatin due to its antifungal mechanism and results, but for fluoride, the results were consistent with those reported by Akhlaghi et al 31 In their study, fluoride had no inhibitory effect on S. mutans. 31 In 2015, de Queiroz et al investigated the ethanolic and methanolic extract of Equisetum hyemale from E. arvense plant.…”

Section: Discussionsupporting

confidence: 91%

“…That was not unexpected for nystatin due to its antifungal mechanism and results, but for fluoride, the results were consistent with those reported by Akhlaghi et al 31 In their study, fluoride had no inhibitory effect on S. mutans. 31 In 2015, de Queiroz et al investigated the ethanolic and methanolic extract of Equisetum hyemale from E. arvense plant. In this study, the researchers investigated the effects of this plant on oral bacteria including S. aureus, S. salivarius, S. sanguis, S. mitis, S. mutans, S. sobrinus, and Lactobacillus casei and found that the plant had a good antibacterial effect on oral bacteria.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

In Vitro Antimicrobial and Cytotoxicity Activities of Some Medicinal Plant Extracts against Oral Microbial Pathogens

Amirinia¹,

rad²,

Pourhajibagher³

2021

View full text Add to dashboard Cite

Introduction: Medicinal plants have long been of great interest to scientists in the search for the best treatment of diseases, especially the infectious diseases. In recent years, the use of herbal medicines has become more well-known because of their antimicrobial, antifungal, anti-cancer and less side effects. Aim: The aim of this study was to investigate the antimicrobial and antifungal effects of Urtica dioica, Equisetum arvense, and Punica Granatum peel extracts on two common oral microorganisms, Streptococcus mutans and Candida albicans. Materials and methods: The study investigated the hydro-alcoholic extract of the plants. The antimicrobial activity of the extracts was evaluated using the method of measuring the inhibition of microorganisms, and the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined using different concentrations of the extracts and also biofilm assay and SEM were determined. Also cell viability was assessed by MTT assay on human gingival fibroblast cells. Results: The lowest MIC against S. mutants and C. albicans was related to the hydro-alcoholic extract of U. dioica. There was a significant reduction in the microbial biofilms by all three extracts. Among them, U. dioica could decrease the biofilms of S. mutans and C. albicans more than other extracts. In addition, the best results for growth inhibition zone were the hydro-alcoholic extracts of E. arvense and U. dioica with 35 and 30 mm growth zone, respectively. The results of SEM showed that P. granatum peel, U. dioica and E. arvense could destroy microbial biofilms without exerting any cytotoxic effects on HGF cell. Conclusions: The results of the study suggest that U. dioica, E. arvense, and P. Granatum peel extracts can be used as mouthwash with the least significant difference with routine mouthwashes. Also, the plant-based mouthwashes may be more suitable substitutes for chemical types in the future.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

In Vitro Antimicrobial and Cytotoxicity Activities of Some Medicinal Plant Extracts against Oral Microbial Pathogens

Amirinia¹,

rad²,

Pourhajibagher³

2021

View full text Add to dashboard Cite

show abstract

“…Coffee is one of the most widely consumed beverages in the world. The use of natural products as part of a daily diet may be a more effective and safer alternative for disease prevention [ 19 ]. We investigated the antibacterial and antibiofilm activities of Robusta coffee extracts against B. cereus and S. aureus , causative agents of foodborne bacterial intoxication.…”

Section: Discussionmentioning

confidence: 99%

Robusta coffee extracts inhibit quorum sensing activity in Chromobacterium violaceum and reduce biofilms against Bacillus cereus and Staphylococcus aureus

et al. 2022

View full text Add to dashboard Cite

Background and Aim: Bacillus cereus and Staphylococcus aureus cause foodborne intoxication in humans and animals. Pathogens can produce biofilms controlled by the quorum sensing system. The study aimed to investigate the antibacterial, antibiofilm, and anti-quorum sensing activities of Coffea canephora P. ex Fr. (Robusta coffee) extracts against B. cereus and S. aureus. Materials and Methods: Ethanol extracts of fruit peels and seeds of Robusta coffee were tested for antibacterial activity against B. cereus and S. aureus using a broth microdilution assay. Reduction of the biofilm formation and elimination of the viability of mature biofilm-grown cells of B. cereus and S. aureus were determined. Inhibition of quorum sensing activity in Chromobacterium violaceum by the extracts was investigated using the disk diffusion method and flask incubation assay. Results: Fresh fruit peel extract showed the strongest antibacterial activity against B. cereus and S. aureus with minimum inhibitory concentration (MIC) values of 2 and 4 mg/mL, respectively. However, the extracts did not inhibit Escherichia coli, avian pathogenic E. coli, and Pseudomonas aeruginosa at 8 mg/mL. Significant inhibition of biofilm formation at 1/2 × MIC of the fresh peel extract was detected in B. cereus (56.37%) and S. aureus (39.69 %), respectively. At 8 × MIC of the fresh peel extract, a significant elimination of the mature biofilm viability was detected in B. cereus (92.48%) and S. aureus (74.49%), respectively. The results showed that fresh and dried peel fruit extracts at 1/2 × MIC significantly reduced violacein production with the highest percentage inhibition ranging from 44.53 to 47.48% at 24 h (p ≤ 0.05). Conclusion: The results of the present study suggest the potential therapeutic benefits of Robusta coffee extracts in inhibiting the growth, biofilm, and quorum sensing of both B. cereus and S. aureus. The results put forward an alternative strategy to control the foodborne intoxications caused by both pathogens.

show abstract

“…Senyawa kimia yang terkandung dalam kopi menyebabkan ekstrak kopi memiliki aktivitas antimikroba (58). Senyawa kimia yang memberikan efek antimikroba pada ekstrak kopi adalah senyawa polifenol (59). Senyawa polifenol yang memberikan efek antimikroba diduga bekerja dengan cara menyebabkan perubahan permeabilitas membran bakteri (59).…”

Section: Berdasarkanunclassified

Pengaruh Antioksidan dan Kombinasi Pengawet terhadap Stabilitas Ekstrak Cair NADES Biji Kopi Hijau

Ramadon

Septiani

Putri

et al. 2021

JFI

View full text Add to dashboard Cite

Biji kopi hijau (Coffea canephora Pierre ex A. Froehner) mengandung senyawa kafein dan asam klorogenat yang bermanfaat bagi kesehatan. Dua kandungan utama tersebut dapat diekstraksi menggunakan pelarut Natural Deep Eutectic Solvents (NADES) yang dikombinasi dengan metode Ultrasound-Assisted Extraction (UAE) yang bersifat ramah lingkungan. NADES dapat digunakan untuk mengekstraksi biji kopi hijau, namun pelarutnya tidak dapat diuapkan sehingga hasil akhir yang didapatkan berupa ekstrak cair. Ekstrak cair selama penyimpanan rentan mengalami oksidasi dan pertumbuhan bakteri sehingga dapat mengganggu stabilitas fisik, kimia, dan mikrobiologi. Pada penelitian ini, dilakukan penambahan butil hidroksi toluen (BHT) sebagai antioksidan sintetik dan kombinasi metil paraben-propil paraben sebagai pengawet untuk mengetahui pengaruhnya terhadap stabilitas fisik, kimia, dan mikrobiologi ekstrak cair NADES biji kopi hijau, serta untuk mengetahui suhu penyimpanan yang paling optimal. Konsentrasi BHT yang ditambahkan pada penelitian ini adalah 10, 20, dan 30 ppm. Konsentrasi kombinasi metil paraben:propil paraben yang ditambahkan adalah 110:55 ppm, 100:50 ppm, dan 90:45 ppm. Masing-masing ekstrak disimpan pada suhu penyimpanan -20°C ± 5°C, 5°C ± 3°C, dan 30°C ± 2°C selama 54 hari. Hasil penelitian menunjukkan bahwa ekstrak cair yang ditambahkan BHT konsentrasi 30 ppm dan kombinasi pengawet konsentrasi 110:55 ppm serta disimpan pada suhu penyimpanan -20°C ± 5°C memiliki stabilitas fisik, kimia, dan mikrobiologi paling optimal. Hal ini menunjukkan bahwa penambahan BHT dan kombinasi pengawet dapat meningkatkan stabilitas ekstrak NADES biji kopi hijau.

show abstract

The antibacterial effects of coffee extract, chlorhexidine, and fluoride against Streptococcus mutans and Lactobacillus plantarum: An in vitro study

Cited by 16 publications

References 20 publications

In Vitro Antimicrobial and Cytotoxicity Activities of Some Medicinal Plant Extracts against Oral Microbial Pathogens

In Vitro Antimicrobial and Cytotoxicity Activities of Some Medicinal Plant Extracts against Oral Microbial Pathogens

Robusta coffee extracts inhibit quorum sensing activity in Chromobacterium violaceum and reduce biofilms against Bacillus cereus and Staphylococcus aureus

Pengaruh Antioksidan dan Kombinasi Pengawet terhadap Stabilitas Ekstrak Cair NADES Biji Kopi Hijau

Contact Info

Product

Resources

About