The antibacterial activity of Magainin I immobilized onto mixed thiols Self-Assembled Monolayers

Humblot, Vincent; Yala, Jean-Fabrice; Thébault, Pascal; Boukerma, Kada; Héquet, Arnaud; Berjeaud, Jean‐Marc; Pradier, Claire‐Marie

doi:10.1016/j.biomaterials.2009.03.025

Cited by 178 publications

(172 citation statements)

References 64 publications

Supporting

Mentioning

168

Contrasting

Unclassified

Order By: Relevance

“…Haynie et al [67] was able to demonstrate that immobilized magainin that is attached through very short linkers (2 or 6 carbon atoms) displays antimicrobial activity, meaning that outer membrane interaction by magainin is sufficient for lethal activity. Humblot et al [68] reported that the immobilized AMPs had a bacteriostatic rather that bactericidal effect, perhaps due to the low peptide concentration or short contact time. Other research has performed the immobilization through long linkers in an attempt to permit sufficient flexibility to penetrate target cell membranes [1,69,70], as the directly attached AMPs lost their antimicrobial activity.…”

Section: Mode Of Antibacterial Actionmentioning

confidence: 99%

“…Other important activity-modulating parameters include the length, flexibility and kind of spacer between the active sequences and the solid matrices [67,69,70], the AMP surface density [1,68,69], and orientation after immobilization [1,11,[67][68][69][70][76][77][78]. These parameters are discussed below.…”

Section: Covalent Immobilization Of An Antimicrobial Peptidementioning

confidence: 99%

“…silanized titanium) [70], glass coverslips [76], model surfaces (e.g. self-assembled monolayers) [68], microtitre plates [71] and even commercial contact lenses [11].…”

Section: Solid Supports and Chemical Coupling Strategiesmentioning

confidence: 99%

“…Although some studies demonstrated that immobilized AMPs have antimicrobial activity without incorporation of a spacer [11,68,71,76], most protocols presented a spacer attachment step [1,56,67,69,70,77,78], particularly with a PEG spacer with a MW ranging from 3000 to 5400 [56,69,70,77,78]. The utilization of PEG as a spacer presents several advantages.…”

Section: Influence Of the Spacermentioning

confidence: 99%

“…In this study, the higher concentration of the peptide via ABA immobilization correlated with a more profound antibacterial activity. Humblot et al [68] correlated low peptide concentrations with bacteriostatic rather than bacteriocidal effect of immobilized magainin I, explaining that the low peptide concentration precludes the possibility of multiple peptide entries into the cell membrane. In addition, Hilpert et al [71] studied the effect of immobilized peptide density on antimicrobial activity by utilizing different concentrations of biotinylated peptide solutions with streptavidin-coated plates.…”

Section: Peptide Concentrationmentioning

confidence: 99%

See 4 more Smart Citations

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

et al. 2011

View full text Add to dashboard Cite

a b s t r a c tBacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs' potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and longterm stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices.

show abstract

Section: Mode Of Antibacterial Actionmentioning

confidence: 99%

Section: Covalent Immobilization Of An Antimicrobial Peptidementioning

confidence: 99%

“…silanized titanium) [70], glass coverslips [76], model surfaces (e.g. self-assembled monolayers) [68], microtitre plates [71] and even commercial contact lenses [11].…”

Section: Solid Supports and Chemical Coupling Strategiesmentioning

confidence: 99%

Section: Influence Of the Spacermentioning

confidence: 99%

Section: Peptide Concentrationmentioning

confidence: 99%

See 3 more Smart Citations

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

et al. 2011

View full text Add to dashboard Cite

show abstract

Polymer Brushes

Hadjesfandiari

Bajgai

et al. 2013

Encyclopedia of Polymer Science and Technology

View full text Add to dashboard Cite

Polymer brushes are monolayers of one‐end tethered polymer chains at high graft density on a surface. Owing to the steric interactions between the tethered polymer chains within the brush regime, this class of polymer thin films has unique properties compared to the conventional polymer thin films. Polymer brushes provide an elegant route for surface modification owing to their excellent mechanical stability as well as functional versatility. Among the polymer brush synthesis methods, surface‐initiated polymerization (SIP) offers a unique opportunity for the generation of high graft density polymer brushes. Combined with controlled/living polymerization, the SIP method revolutionized the polymer brush synthesis and allowed the tailoring of surface properties of these molecular thin films. The control of surface properties provided new ways to change wettability, lubrication, and the interaction of biological macromolecules/cells to surfaces and created new opportunities in the design of responsive surfaces, smart materials, biocompatible surfaces, and various biotechnology and nanotechnology applications. This field is rapidly emerging with wide range of applications in multiple research fields and is promoting cross‐disciplinary research.

show abstract

Antimicrobial Peptides

Marcos¹,

Manzanares²

2011

Antimicrobial Polymers

View full text Add to dashboard Cite

Antimicrobial peptides (AMPs), important effector molecules of the innate immune system, also provide templates for designing novel antibiotics. Protegrin, an especially potent AMP found in porcine leukocytes, was recently shown to form octameric transmembrane pores. We have employed a combination of experiments and models spanning length scales from the atomistic to the cellular level in order to elucidate the microbicidal mechanism of protegrin. Comparison of the modeling and experimental data suggests that approximately 10-100 protegrin pores are necessary to explain the observed rates of potassium leakage and Escherichia coli death in exponential-phase bacteria. The kinetics of viability loss suggest that bacterial death results largely from uncontrolled ion exchange processes and decay of transmembrane potential. However, ion exchange processes alone cannot account for the experimentally observed cell swelling and osmotic lysis-a redundant ''overkill'' mechanism most likely to occur in locales with high protegrin concentrations. Although our study is limited to protegrin and E. coli, the timeline of events described herein is likely shared by other AMPs that act primarily by permeabilizing microbial membranes. This work provides many of the missing links in describing antimicrobial action, as well as providing a quantitative connection between several previous experimental and simulation studies of protegrin.

show abstract

The antibacterial activity of Magainin I immobilized onto mixed thiols Self-Assembled Monolayers

Cited by 178 publications

References 64 publications

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

Polymer Brushes

Antimicrobial Peptides

Contact Info

Product

Resources

About