The Anti-proliferation Effect of an Isolated Butanol Fraction of Tampa Badak (Voacanga foetida (Bl.) K. Schum) Leaves on Leukemia, Lung, and Cervical Cancer

Susanty, Adriani; Dachriyanus, Dachriyanus; Yanwirasti, Yanwirasti; Wahyuni, Fatma Sri; Sekar, Andisyah Putri; Alimin, Nur; Magdazaleni, Magdazaleni; Sofia, Sri Esky; Dewi, Citra Kartika

doi:10.7454/psr.v7i3.1196

Cited by 5 publications

(5 citation statements)

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“…Ekstrak etanol daun V. foetida memiliki efek analgesik pada dosis 250 mg/kg BB [8], ekstrak etil asetat dari daun V. foetida memiliki aktivitas sitotoksik pada sel kanker usus (HTB-38) dengan nilai IC 50 sebesar 0,1 g/mL [9], senyawa isolat Tb3 dari daun V. foetida memiliki efek antiproliferasi pada kanker leukemia (K562), paru (A549) dan serviks (He-La) dengan nilai IC 50 berturut-turut adalah 0,5; 2,4 dan 3,6 g/mL dibandingkan doksorubisin sebagai kontrol positif dengan nilai IC 50 0,2 g/mL [10], dan senyawa hasil isolasi (vobtusin) dari kulit batang tumbuhan V. foetida pada dosis 20 µM dapat menginduksi apoptosis melalui jalur mitokondria dengan cara mengaktivasi caspase-3 dan caspase-9 pada sel kanker leukemia HL-60 pada [2].…”

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“…Hasil penelitian ini didukung dengan penelitian kami sebelumnya [9], yaitu ekstrak etil asetat dari daun V. foetida memiliki aktivitas sitotoksik kuat pada sel HTB-38 dengan nilai IC 50 sebesar 0,1 µg/mL. Hasil penelitian ini juga sejalan dengan penelitian yang dilakukan oleh Susanty et al, 2020, senyawa isolat Tb3 dari V. foetida memiliki efek antiproliferasi pada sel leukemia (K526), kanker paruparu (A549), dan kanker serviks (He-La) dengan nilai IC 50 adalah 0,5; 2,4, dan 3,6 g/mL [10].…”

Section: Hasil Dan Diskusiunclassified

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In Vitro Cytotoxic Activity Test of Tampa Badak (Voacanga foetida (Blume) Leaves on L1210 Leukemia

Susanty¹

2021

J Sains Farm Klin

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Based on previous research, the ethanolic extract of the Voacanga foetida (Blume) Rolfe plant has cytotoxic activity using the Brine Shrimps Lethality test. Therefore, further tested the anticancer activity of extracts, fractions and isolated compounds from V. foetida leaves in vitro on L1210 leukemia cells using the direct cell count method with positive control doxorubicin. The results showed that the methanol extract, fraction of butanol fraction, ethyl acetate, and hexane, as well as isolated compounds Tb1 from fraction of hexane, Tb2 from fraction of ethyl acetate, and Tb3 from fraction of butanol leaves from V. foetida, had IC50 values of 4,4; 9; 8,4; 16,8; 0,9; 1,8; and 1,1 µg/mL, while doxorubicin has an IC 50 value of 0.2 µg/mL. Based on the IC 50 value, it showed that the extract, fraction, and isolated compound from Voacanga foetida leave had a strong cytotoxic effect as evidenced by the IC50 value < 20 µg/mL (extract and fraction) and < 4 µg/mL (isolate) for each test concentration.

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Section: Hasil Dan Diskusiunclassified

In Vitro Cytotoxic Activity Test of Tampa Badak (Voacanga foetida (Blume) Leaves on L1210 Leukemia

Susanty¹

2021

J Sains Farm Klin

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“…Based on our previous work, the leaves methanolic extracts, their fractions (n-hexane, ethyl acetate, and butanol fractions), and some isolated compounds from this plant were reported to have highly cytotoxic activity against L1210 blood cancer cells 10 . One isolated compound from this plant's butanol fraction of leaves ethanolic extract was also tested against various cancer cell lines (K562, A549, HeLa), and the isolate exhibited a highly cytotoxic activity 11 . In addition, the ethanolic extract also demonstrated in vivo anticarcinogenic activity on the dose of 200 mg/kgBW, and based on the acute toxicity test, this extract was also categorized as not toxic (LD50 >15000 mg/kgBW) 12 .…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Activity of Ethyl Acetate Extract from Voacanga foetida (Bl.) Rolfe Leaves Against T47D Breast Cancer Cells

et al. 2023

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The cytotoxicity of ethyl acetate extract from Tampa badak (Voacanga foetida (Bl.) Rolfe) leaves against Artemia salina leach larvae was determined using the brine shrimp lethality test (BSLT) method and was evaluated against T47D breast cancer cells using MTT assay method. The result of BSLT showed a consistent result with MTT assay, which is the result obtained that ethyl acetate extract is very toxic against A. salina Leach larvae with LC50 value of 8.61 µg/mL and very cytotoxic against T47D breast cancer cells with IC50 values of 0.87; 0.66; and 0.95 µg/mL at the 24, 48 and 72 hours of incubation times, respectively. The MTT assay data were analyzed using a two-way ANOVA statistical method to see the effect of the dependent variable (concentration and time) on the independent variable (% viability). Based on the statistical test result, there is a difference in % viability between concentrations of 0.1; 1; and 10 µg/mL (p <0.05), but the length of incubation does not affect % viability (p >0.05).

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“…In addition, n-hexane, ethyl acetate, n-butanol fractions and several isolated compounds from V. foetida leaves were reported to have good cytotoxic activity against L1210 leukemia cell lines [10]. Then, one isolated compound from n-butanol fraction of V. foetida leaves also exhibited anti-proliferation activities against several cell lines including leukemia, lung, and cervical cancers [11].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Cytotoxic Activity Test of Alkaloids from Dichloromethane Fraction of Bark of Tampa Badak (Voacanga foetida (Blume) Rolfe) Against T47D Cell Line

Susanty,

Putri,

Ikhtiarudin

et al. 2023

J. Trop. Pharm. Chem.

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Cancer is a problem in the health sector because this disease has an increasing incidence of morbidity and mortality. While the treatment still leaves some drug side effects, which cause discomfort for cancers sufferers. The arises because most cancer drugs work non-selectively. Because of some of the problems above, it is necessary to encourage the search for alternative drugs treatment of cancer. Voacanga foetida (Blume) Rolfe (Apocynaceae) is a species of plant from the genus Voacanga known as the tampa badak from West Sumatra. This plant is known to be source of indole alkaloids and also bis-indole alkaloids potential as cancer drugs. Therefore, in this study we are interested to explore the potency of this plant as natural product sources with potential cytotoxic activity. The study was started from the isolation process and continued by characterization of the isolated compounds and then testing their cytotoxic activity T47D breast cancer cell line. The isolation process of alkaloid compounds were performed by acid-base extraction, liquid-liquid fractionation, and followed by separation using column chromatography. Then,the potency of cytotoxic activity of the isolates were screened through Brine Shrimp Lethality Test (BSLT) method and evaluated by MTT assay. The structure of three isolated alkaloids (VFB-DB1.1; VFB-DB2.1; and VFB-DB2.2) were charachterized using spectroscopic analyses, including UV and FT-IR. One isolated compound (VFB-DB1.1) was also characterized using 1H NMR spectroscopy. Based on the similarity of 1H NMR spectra of isolated compound when compared with the literature, compound VFB-DB1.1 was identified as voacangine. Then, the BSLT and MTT assay result showed that VFB-DB1.1 exhibited LC50 value of 15.8 µg/mL againt Artemia salina L.and IC50 value of 8.9 µg/mL against T47D breast cancer cells, respectively.

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The Anti-proliferation Effect of an Isolated Butanol Fraction of Tampa Badak (Voacanga foetida (Bl.) K. Schum) Leaves on Leukemia, Lung, and Cervical Cancer

Cited by 5 publications

References 14 publications

In Vitro Cytotoxic Activity Test of Tampa Badak (Voacanga foetida (Blume) Leaves on L1210 Leukemia

In Vitro Cytotoxic Activity Test of Tampa Badak (Voacanga foetida (Blume) Leaves on L1210 Leukemia

Cytotoxic Activity of Ethyl Acetate Extract from Voacanga foetida (Bl.) Rolfe Leaves Against T47D Breast Cancer Cells

Isolation and Cytotoxic Activity Test of Alkaloids from Dichloromethane Fraction of Bark of Tampa Badak (Voacanga foetida (Blume) Rolfe) Against T47D Cell Line

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