The anti-osteoporosis effects of CSN1S2 protein of goat milk and yoghurt on a complete Freund's adjuvant-induced rheumatoid arthritis model in rats

Fatchiyah, Fatchiyah; Setiawan, Bambang; Suharjono, Suharjono; Noor, Zairin

doi:10.1016/j.bgm.2015.10.001

Cited by 19 publications

(20 citation statements)

References 38 publications

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“…At a dose of 2 mg/kg body weight, CSN1S2 from goat milk is able to decrease the severity scoring, TNF-a, and RAGE expression in a complete Freund's adjuvant induced rheumatoid arthritis model in rats. Besides, this compound also acts as antiosteoporotic agent [6,12,15]. In this study, acute and chronic toxicity testing of male and female rats treated with caprine CSN1S2 protein from fresh milk was conducted.…”

Section: Discussionmentioning

confidence: 99%

“…The biochemical parameters measured were: aspartate aminotransferase (AST, IU/dL), alanine aminotransferase (ALT, IU/dL), alkaline phosphatase (ALP, IU/dL), blood urea nitrogen (BUN, mg/dL), creatinine (mg/dL), glucose (mg/dL), total cholesterol (TCHO, mg/dL), total protein (g/dL), triglyceride (TG, mg/dL), uric acid (mg/dL), and bilirubin (mg/dL). The hematological parameters measured were: white blood cells (WBC) (x10 3 /µL), platelet (x10 3 /µL), red blood cell (RBC) (x10 6 /µL), hemoglobin (g/dL), hematocrit (%), mean corpuscular volume (MCV) (fL), mean corpuscular hemoglobin (MCH) (pg), and mean corpuscular hemoglobin concentration (MCHC) (g/dL).…”

Section: Hematology and Serum Biochemistrymentioning

confidence: 99%

“…Goat milk protein is provided as healthy nutrition and as a therapeutic agent to control abnormal or disease conditions through some of its biologically active peptide residues [4]. Alpha-S2 casein (CSN1S2) protein of goat milk has a biological function due to its anti-oxidant, antiosteoporotic, anti-inflammatory activities, or as an immunomodulatory agent to prevent immune system failure and homeostasis of metabolic mechanisms, respectively [4][5][6]. Another study explained that goat milk supplementation was also able to protect and reduce the severity of carcinogenesis in hepatocarcinogenesis-induced rats [7].…”

Section: Introductionmentioning

confidence: 99%

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Safe Dosage of Caprine CSN1S2 Protein from Fresh Local Goat Milk for Rats Evaluated by Acute and Sub-Chronic Toxicity Testing

Fatchiyah

Setiawan

Ohta

et al. 2017

J. Math. Fund. Sci.

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Abstract. Caprine milk CSN1S2 protein contains bioactive peptides that have a specific function in regulating the metabolic homeostasis mechanism between different organs. However, the safe dosage of goat milk consumption has not been established previously. The aim of this study was to evaluate the safe dosage of caprine CSN1S2 protein from fresh local goat milk using acute and repeated doses (for 4 weeks) in oral toxicity studies on three month old normal Wistar rats. The CSN1S2 protein was isolated from fresh goat milk. This casein protein administered in a single oral gavage dose to 5 rat groups at a daily dose of 0, 500, 1000, 2000, 4000 mg/kg BW for acute and 28-day sub-chronic toxicity testing of rats of both sexes. The result showed no evident mortality or abnormal effects. Data analysis of body weight, water and food intake, blood biochemical parameters, clinical observation and organ weight revealed no significant differences between all groups for both sexes. The weights of some organs showed differences between groups, but these values were within the normal weight ranges. The conclusion is that caprine CSN1S2 from fresh milk is safe for food consumption because there was no indication of toxicity, no mortality, and no hematological and clinical chemistry changes.

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Section: Discussionmentioning

confidence: 99%

Section: Hematology and Serum Biochemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Safe Dosage of Caprine CSN1S2 Protein from Fresh Local Goat Milk for Rats Evaluated by Acute and Sub-Chronic Toxicity Testing

Fatchiyah

Setiawan

Ohta

et al. 2017

J. Math. Fund. Sci.

Self Cite

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“…A paper by Budiarti et al [108] demonstrates the presence of Alpha-S2 casein in Ethawah goat milk and yoghurt. This protein contains eight bioactive peptides, with different effects, such as anti-osteoporotic or anti-inflammatory effects, and it was not found in cow fresh milk [109,110]. More recently, Triprisila et al [111] reported the effect of antimicrobial activity from CSN1S2 protein as a member of casein protein from Ethawah breed goat milk and yoghurt against pathogen Gram-positive bacteria (Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus) and Gram-negative bacteria (Escherichia coli, Salmonella typhi, and Shigella flexneri).…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

Nutritional and Health Profile of Goat Products: Focus on Health Benefits of Goat Milk

Lima¹,

Teixeira-Lemos²,

Oliveira³

et al. 2018

Goat Science

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Goat (Capra hircus) is one of the main sources of milk and meat products for human consumption. Goat milk differs from cow and human milk in both composition and nutritional properties. Goat milk and other goat-derived products contain several bioactive compounds that might be useful in patients suffering from a variety of chronic diseases. Several peptides, fats, and oligosaccharides present in goat's milk can be potentially useful in cardiovascular disease, metabolic disorders, neurological degeneration, or in promoting intestinal health. They have also shown chemopreventive properties in cancer. In addition, the oligosaccharides present in goat's milk have immunomodulatory properties, prevent adhesion of pathogenic bacteria, and have prebiotic, probifidogenic effects. Due to its potential health benefits, goat milk is particularly recommended for infants, older adults, and convalescing people. This chapter gives an overview of the biological activities of goat products and the effects of peptides, fats, and oligosaccharides present in goat milk on pathogenic bacteria, as well as their ability to regulate immunological, gastrointestinal, hormonal, and neurological responses in humans.

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“…CSN1S2 is known as an inducible molecule for proliferation 17 of pre-osteoblast cells that form the microstructure of the femur. 18 Protein CSN1S2 has not just the potential for proliferation of immunocompetent cells, however, it could provide potential nutrition for inflammation. …”

Section: -2mentioning

confidence: 99%

Influence of CSN1S2 protein from Caprine milk Etawah Breed (EB) on histology of microglial cells in rat (Rattus norvegicus) Type-2 diabetes mellitus (T2DM)

Rika

Fatchiyah

2017

AIP Conference Proceedings

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The improvement of the quality of polluted irrigation water through a phytoremediation process in a hydroponic batch culture system AIP Conference Proceedings 1908, 030003 (2017) Abstract. Type-2 diabetes mellitus (T2DM) is a degenerative disease that causes an imbalance in the metabolism. The aim of this research is to determine the influences of CSN1S2 on the structure of microglial cells in T2DM. Rats (Rattus norvegicus) were divided into eight groups of treatment with looping three times each between treatment groups (CM) Control. The control is given a milk treatment with doses of 375 mg/kg (CM375), 750 mg/kg (CM750), and 1500 mg/kg (CM1500), T2DM (DMK), and T2DM with CSN1S2 375 mg/kg dose (DM375), 750mg/kg (DM750), and 1500 mg/kg (DM1500). The animal model T2DM was induced by a high-fat diet in the form of feed followed by injection of STZ (dose of 25 mg/kg of animal treatment) and treatment of CSN1S2 for 28 days. Brain organs were taken and analysed in histopathology stained by Hematoxylin-eosin (HE) and observed using Olympus BX53. Based on the results, it was concluded that CSN1S2 protein is influential for induction of microglial cell proliferation in animal models of T2DM, as immunity responds to the inflammatory condition in T2DM.

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The anti-osteoporosis effects of CSN1S2 protein of goat milk and yoghurt on a complete Freund's adjuvant-induced rheumatoid arthritis model in rats

Cited by 19 publications

References 38 publications

Safe Dosage of Caprine CSN1S2 Protein from Fresh Local Goat Milk for Rats Evaluated by Acute and Sub-Chronic Toxicity Testing

Safe Dosage of Caprine CSN1S2 Protein from Fresh Local Goat Milk for Rats Evaluated by Acute and Sub-Chronic Toxicity Testing

Nutritional and Health Profile of Goat Products: Focus on Health Benefits of Goat Milk

Influence of CSN1S2 protein from Caprine milk Etawah Breed (EB) on histology of microglial cells in rat (Rattus norvegicus) Type-2 diabetes mellitus (T2DM)

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