The Anti-Inflammatory, Phytoestrogenic, and Antioxidative Role of<i>Labisia pumila</i>in Prevention of Postmenopausal Osteoporosis

Nadia, M. E.; Nazrun, A. S.; Norazlina, M.; Isa, Nurulhikma Md; Muhammad, Norliza; Nirwana, S Ima

doi:10.1155/2012/706905

Cited by 28 publications

(35 citation statements)

References 77 publications

(66 reference statements)

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“…This is in line with the fact that estrogen is able to offer antioxidant protection of lipoproteins and increase the expression of these enzymes in bone cells [56]. On the other hand, LP stimulated the antioxidant enzymes activity and decreased the MDA level via its phytoestrogenic and antioxidative properties [22]. Our findings may also be explained by free radical scavenging properties of LP as reported in the previous study [46].…”

Section: Discussionsupporting

confidence: 91%

Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis Rat Model

Effendy

Shuid

2014

Nutrients

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Postmenopausal osteoporosis can be associated with oxidative stress and deterioration of antioxidant enzymes. It is mainly treated with estrogen replacement therapy (ERT). Although effective, ERT may cause adverse effects such as breast cancer and pulmonary embolism. Labisia pumila var. alata (LP), a herb used traditionally for women’s health was found to protect against estrogen-deficient osteoporosis. An extensive study was conducted in a postmenopausal osteoporosis rat model using several LP doses and duration of treatments to determine if anti-oxidative mechanisms were involved in its bone protective effects. Ninety-six female Sprague-Dawley rats were randomly divided into six groups; baseline group (BL), sham-operated (Sham), ovariectomised control (OVXC), ovariectomised (OVX) and given 64.5 μg/kg of Premarin (ERT), ovariectomised and given 20 mg/kg of LP (LP20) and ovariectomised and given 100 mg/kg of LP (LP100). The groups were further subdivided to receive their respective treatments via daily oral gavages for three, six or nine weeks of treatment periods. Following euthanization, the femora were dissected out for bone oxidative measurements which include superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) levels. Results: The SOD levels of the sham-operated and all the treatment groups were significantly higher than the OVX groups at all treatment periods. The GPx level of ERT and LP100 groups at the 9th week of treatment were significantly higher than the baseline and OVX groups. MDA level of the OVX group was significantly higher than all the other groups at weeks 6 and 9. The LP20 and LP100 groups at the 9th week of treatment had significantly lower MDA levels than the ERT group. There were no significant differences between LP20 and LP100 for all parameters. Thus, LP supplementations at both doses, which showed the best results at 9 weeks, may reduce oxidative stress which in turn may prevent bone loss via its anti-oxidative property.

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Section: Discussionsupporting

confidence: 91%

Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis Rat Model

Effendy

Shuid

2014

Nutrients

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“…Estrogen is able to suppress the production of proinflammatory cytokines like IL-1, IL-6, IL-7 and TNF-α. This is why these cytokines are elevated in postmenopausal women [8]. Treatment focuses on slowing down or stopping the mineral loss, thereby preventing bone fractures and controlling the pain associated with the disease.…”

Section: Introductionmentioning

confidence: 99%

Experimental techniques for screening of antiosteoporotic activity in postmenopausal osteoporosis

Satpathy

Patra

Ahirwar

2015

Journal of Complementary and Integrative Medicine

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Postmenopausal osteoporosis, a silent epidemic, has become a major health hazard, afflicting about 50% of postmenopausal women worldwide and is thought to be a disease with one of the highest incidences in senile people. It is a chronic, progressive condition associated with micro-architectural deterioration of bone tissue that results in low bone mass, decreased bone strength that predisposes to an increased risk of fracture. Women are more likely to develop osteoporosis than men due to reduction in estrogen during menopause which leads to decline in bone formation and increase in bone resorption activity. Estrogen is able to suppress the production of proinflammatory cytokines like interleukin (IL)-1, IL-6, IL-7 and tumor necrosis factor (TNF-α). This is why these cytokines are elevated in postmenopausal women. In this review article we have made an attempt to collate the various methods and parameters most frequently used for screening of antiosteoporotic activity in postmenopausal osteoporosis. Pertaining to ovariectomized animal model, this is the most appropriate model for studying the efficacy of different drugs to prevent bone loss in postmenopausal osteoporosis.

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“…This explains the anti-inflammatory ability of LP in inhibiting TNF-α and COX-2. Hence, the possible mechanisms contributing to the osteoprotective effects of LP are via its antioxidative, phytoestrogenic and anti-inflammatory properties [92].…”

Section: Discussionmentioning

confidence: 99%

The effects of Labisia pumila on postmenopausal osteoporotic rat model: Dose and time-dependent micro-CT analysis

Effendy

Khamis

Soelaiman

et al. 2014

Journal of X-Ray Science and Technology

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BACKGROUND: Postmenopausal osteoporosis is best treated and prevented by estrogen replacement therapy (ERT). Although effective, ERT may cause breast cancer, uterine cancer and cardiovascular problems. Labisia pumila var. alata (LP), a herb with phytoestrogenic, antioxidative and anti-inflammatory effects has potential as an ERT alternative. OBJECTIVE: This study aimed to evaluate micro-CT analysis on the effects of LP supplementation on the trabecular microarchitecture of postmenopausal osteoporosis rat model. Micro-CT is an effective tool in detecting changes in trabecular bone structure and providing a three dimensional information which may replace other conventional bone analysis methods. METHODS: Ninety-six female Sprague-Dawley rats (4 to 5 months old) were randomly divided into six groups of baseline group (BL) Sham-operated (Sham), ovariectomised control (OVXC), ovariectomised with 64.5 μg/kg of Premarin (ERT), ovariectomised with 20 mg/kg of LP (LP20) and ovariectomised with 100 mg/kg of LP at (LP100). The vehicle (deionized water), Premarin and LP were given via daily oral gavages for three, six and nine weeks of treatment periods. Rats in BL group were euthanized before the start of the study, while other rats were euthanized after completion of their treatments. Femora were dissected out and trabecular bone microarchitecture analysed with micro-CT. RESULTS: Micro-CT analysis of OVXC rats revealed significant osteoporotic changes in connectivity density, trabecular bone volume, trabecular thickness, trabecular separation and trabecular number. Both ERT and LP were able to reverse all the OVX-induced bone changes with the best results seen with 100 mg/kg of LP for nine weeks duration of treatment. CONCLUSION: Micro-CT provides accurate and reliable information on trabecular bone parameters which aid in the diagnosis and treatment of osteoporosis. LP supplementation at 100 mg/kg was more effective than ERT in reversing ovariectomyinduced bone changes. Further studies are required to explore the potential of LP as ERT alternative in the treatment and prevention of postmenopausal osteoporosis.

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The Anti-Inflammatory, Phytoestrogenic, and Antioxidative Role ofLabisia pumilain Prevention of Postmenopausal Osteoporosis

Cited by 28 publications

References 77 publications

Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis Rat Model

Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis Rat Model

Experimental techniques for screening of antiosteoporotic activity in postmenopausal osteoporosis

The effects of Labisia pumila on postmenopausal osteoporotic rat model: Dose and time-dependent micro-CT analysis

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