The Anti-inflammatory Effect of Z-Ligustilide in Experimental Ovariectomized Osteopenic Rats

Abstract

The purpose of the present study was to investigate the anti-inflammatory activity of Z-Ligustilide (LIG) in experimental ovariectomized (OVX) osteopenic rats. The anti-inflammatory potential of LIG in the regulation of nuclear factor kappa B (NF-κB), maleic dialdehyde (MDA), polymorphonuclear cells (PMN), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS) and tumor necrosis factor-α (TNF-α), adhesion molecule (ICAM-1) and cyclooxygenase-2 (COX-2) was determined by ELISA. LIG significantly inhibited OVX-induced up-regulation of NF-κB activation and the production of IL-1β, TNF-α, iNOS, ICAM-1 and COX-2. Moreover, LIG suppressed MDA and infiltration of PMN. The results of the present study clearly demonstrate that there may be an inflammatory component in the etiology of osteoporosis. It revealed a significant anti-inflammatory effect of Z-Ligustilide in experimental OVX osteopenic rats.

Anti-Osteoporotic Effects of Angelica sinensis (Oliv.) Diels Extract on Ovariectomized Rats and Its Oral Toxicity in Rats

“…It has been demonstrated that oxidation-derived free radicals increase bone resorption by promoting osteoclastic differentiation [ 31 ]. Ma et al demonstrated that Z-ligustilide from the essential oil of A. sinensis inhibits the OVX-induced serum interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels [ 32 ]. IL-1β is known as a highly potent bone-resorptive cytokine [ 9 ] and TNF-α appears to synergize with IL-1 to increase bone resorption [ 11 ].…”

Combining Chemical Profiling and Network Analysis to Investigate the Pharmacology of Complex Prescriptions in Traditional Chinese Medicine

“…Nevertheless, we do not find any reference that directly indicates the association between (Z)-Ligustilide and COX-1. There are relevant studies about the modulation of (Z)-Ligustilide on cyclooxygenase-2 (COX-2)272829, a protein that has similar function as COX-1. However, in our experiments of normal platelets, COX-2 is expected to be little expressed.…”

Effects of Inducible Nitric Oxide Synthase Inhibition on Cardiovascular Risk of Adult Endotoxemic Female Rats: Role of Estrogen

“…OVX rats, in the face of inflammatory challenges, express more iNOS than SHAM rats ( Crisafulli et al, 2009 ; Ma and Bai, 2012 ; Sakanashi et al, 2013 ; Hassouna et al, 2014 ), and estradiol is a known stimulator of NO synthesis, including the increase of iNOS expression and activity ( Nuedling et al, 1999 ; Zhu et al, 2002 ). In addition, progesterone is able to reduce iNOS activity in LPS-stimulated cells ( Su et al, 2007 ; Menzies et al, 2011 ), suggesting that the lower plasma levels of nitrite observed in the SHAM group may be the result of a synergism between iNOS inhibition by SMT and the reduction of the activity of this enzyme by circulating progesterone of these animals.…”