The anti-inflammatory effect of a glycosylation product derived from the high hydrostatic pressure enzymatic hydrolysate of a flatfish byproduct

Abstract

In this study, flatfish byproducts were hydrolyzed by Protamex at high hydrostatic pressure and glycosylated with ribose to utilize the protein of flatfish byproducts as a nutraceutical. We investigated the anti-inflammatory effects of glycosylated fish byproduct protein hydrolysate (GFPH) and its anti-inflammatory mechanisms were elucidated in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophage. The results showed that GFPH suppresses LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) dose-dependently. The enzyme-linked immunosorbent assay (ELISA) kit clearly demonstrated that GFPH significantly reduced the production of pro-inflammatory cytokines such as, interleukin (IL)-6, interleukin (IL)-1β and tumor necrosis factor (TNF)-α, and monocyte chemoattractant protein (MCP)-1. Moreover, GFPH reduced nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) activation. These results indicate that the inhibitory effects of GFPH on LPS-induced NO and PGE2 production might be due to the suppression of the NF-κB and MAPKs signaling pathways. Therefore, these results suggest that flatfish byproducts are latent bioactive resources and GFPH may have potential as a therapeutic agent in the treatment of various inflammatory diseases.

Preparation and Evaluation of New Glycopeptides Obtained by Proteolysis from Corn Gluten Meal Followed by Transglutaminase-Induced Glycosylation with Glucosamine

“…Methyl thiazole tetrazolium (MTT) testing was used to evaluate the CGM peptide samples' cytotoxicity in LO2 cells based on the work by Choe et al [19]. LO2 cell mixtures were seeded into 96-well plates with a density of 1 × 10 5 cells per well and allowed to attach at 37 • C for 6 h. Thereafter, the cells were cultured in the presence of various concentrations of peptide samples (0.005-1.0 mg/mL) for another 24 h. The wells were then washed in phosphate buffer and then incubated with MTT at a final concentration of 0.5 mg/mL for an additional 4 h. The remaining MTT solution was then carefully removed.…”

Flavour Improvement of Protein Hydrolysates Derived from Cephalopods Byproducts Using Maillard Reaction: A Short Review

“…The MRPs against different bacteria strains, such as Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Shigella dysenteriae, and Salmonella typhimurium, is documented (Hauser et al, 2014;Chung et al, 2011). The MRPs of fish gelatin hydrolysates showed specificity towards inhibition of Escherichia coli (Hong et al, 2014 MRPs (Choe et al, 2016). Similarly, MRP developed from unicorn leather jacket gelatin hydrolysate and galactose revealed anti-inflammatory activity by decreasing proinflammatory cytokine expression (interleukin-6 and interleukin-1b) and nitric oxide production in LPS-induced RAW 264.7 cells (Karnjanapratum et al, 2016).…”

Progress in using the drugs based on hydrobionts in treatment of respiratory viral infections and their complications

“…Методом иммунофер-ментного анализа было установлено, что пептид GFPH значительно снижал синтез провоспалитель-ных цитокинов (IL-6, IL-1β) и фактора некроза опухо-лей (TNF). Кроме того, данный гликозилированный пептид подавлял функционирование ядерного фак-тора kB (NF-kB) и сигнальных путей MAPKs за счет снижения активации NF-kappaB и МАРК [11].…”

“…Чрезвычайно важным свойством, присущим ак-тивным пептидам из морских организмов, является их способность тормозить процессы перекисного окисления липидов через подавление выработки оксида азота и ряда специфических простагланди-нов [11], что открывает возможности замены син-тетических антиоксидантов для лечения и профи-лактики инфекций респираторного тракта в составе комплексной терапии [56].…”