The Anti-Inflammatory and Antioxidant Potential of Pistachios (Pistacia vera L.) In Vitro and In Vivo

Abstract

Several reports have demonstrated the effectiveness of pistachio against oxidative stress and inflammation. In this study, we investigate if polyphenols extracts from natural raw shelled pistachios (NP) or roasted salted pistachio (RP) kernels have anti-inflammatory and antioxidant properties at lower doses than reported previously, in both in vitro and in vivo models. The monocyte/macrophage cell line J774 was used to assess the extent of protection by NP and RP pistachios against lipopolysaccharide (LPS)-induced inflammation. Moreover, antioxidant activity of NP and RP was assessed in an in vivo model of paw edema in rats induced by carrageenan (CAR) injection in the paw. Results from the in vitro study demonstrated that pre-treatment with NP (0.01, 0.1 and 0.5 mg/mL) and RP (0.01 and 0.1 mg/mL) exerted a significant protection against LPS induced inflammation. Western blot analysis showed NP reduced the degradation of IκB-α, although not significantly, whereas both NP and RP decreased the TNF-α and IL-1β production in a dose-dependent way. A significant reduction of CAR-induced histological paw damage, neutrophil infiltration and nitrotyrosine formation was observed in the rats treated with NP. These data demonstrated that, at lower doses, polyphenols present in pistachios possess antioxidant and anti-inflammatory properties. This may contribute toward a better understanding of the beneficial health effects associated with consumption of pistachios.

Evaluation of antioxidant, antiinflammatory, and gastroprotective properties of Rubus fruticosus L. fruit juice

“…In fact, the inhibition of proinflammatory responses (NO, iNOS, COX‐2, and PGE 2 ) by polyphenols contained in Mexican blackberry ( Rubus spp) extracts has been widely demonstrated, highlighting a significant correlation between antioxidant capacity and NO inhibition (Cuevas‐Rodríguez et al, ). Thus, the antioxidant and antiinflammatory properties of polyphenols, in agreement with previous studies, could be attributed to the reduction of the nitrosative stress and subsequent formation of NO (Impellizzeri et al, , ; Mandalari et al, , ; Paterniti et al, ).…”

The Conquering influence of the Nano extracts of pomegranate peels and pistachio leaves in amelioration of acute liver failure

“…Surprisingly attenuation of the TAA-induced up regulation of the cytokines TNFα, IL6 together was achieved by pomegranate and pistachio either in normal or in nano forms. These data seem to be parallel with coursodonboydle [86] who reported that increased levels of proinflammatory cytokine IL 6 in rat ileum model necrotizing enterocolitis have been normalized after POM seed oil treatment and also go in accordance with the reports of Grace et al [87] and Paternitiet al [88] , where the first revealed that Pistachio extract significantly reduced the non-mitochondrial oxidative burst in macrophages associated with inflammatory response.While the other attributed the decrease in TNF-α and Interleukins production to the presence of polyphenols in pistachios which possess antioxidant and antiinflammatory properties. These data were demonstrated immunohistochemicaly in Fig.14, AL.…”

The Antioxidant and Anti-Inflammatory Properties of Anacardium occidentale L. Cashew Nuts in a Mouse Model of Colitis

“…Doses were given on the basis of a dose-response study. We started with 30 mg/kg dose based on a previous work on nuts [32].…”