The anti-inflammatory and anti-cancer properties of epigallocatechin-3-gallate are mediated by folate cycle disruption, adenosine release and NF-κB suppression

Navarro-Perán, Enma; Cabezas‐Herrera, Juan; Sánchez‐del‐Campo, Luis; Garcı́a-Cánovas, Francisco; Rodríguez-López, JoséNeptuno

doi:10.1007/s00011-008-8013-x

Cited by 48 publications

(32 citation statements)

References 36 publications

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“…It is noteworthy that inhibition of AMPK rescued the EGCG-induced suppression of NF-kB and AP-1 pathways, suggesting that EGCG-activated AMPK suppresses the activity of the NF-kB and AP-1 pathways. Consistent with these observations, an increasing body of evidence demonstrates that AMPK inhibits the NF-kB pathway as well as the inflammatory response in the different types of cells (Navarro-Peran et al, 2008;Green et al, 2011;Morizane et al, 2011). It is also remarkable that EGCG decreases IL-1a in HaCaT keratinocytes, based on the fact that IL-1a induces hypercornification of the infundibulum in a manner similar to that seen in comedones (Guy et al, 1996).…”

Section: Discussionmentioning

confidence: 67%

Epigallocatechin-3-Gallate Improves Acne in Humans by Modulating Intracellular Molecular Targets and Inhibiting P. acnes

Yoon

Kwon

Min

et al. 2013

Journal of Investigative Dermatology

132

106

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Acne vulgaris is a highly prevalent skin disorder characterized by hyperseborrhea, inflammation, and Propionibacterium acnes overgrowth. Only isotretinoin and hormonal therapy reduce sebum production. To identify a new drug candidate that modulates sebum, we examined the effects of EGCG, the major polyphenol in green tea, on human SEB-1 sebocytes and in patients with acne. In SEB-1 sebocytes, we found that EGCG reduced sebum by modulating the AMPK-SREBP-1 signaling pathway. EGCG also reduces inflammation by suppressing the NF-κB and AP-1 pathways. EGCG also induces cytotoxicity of SEB-1 sebocytes via apoptosis and decreases the viability of P. acnes, thus targeting almost all the pathogenic features of acne. Finally, and most importantly, EGCG significantly improved acne in an 8-week randomized, split-face, clinical trial, and was well tolerated. Our data provide a therapeutic rationale for the use of EGCG in acne.

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Section: Discussionmentioning

confidence: 67%

Epigallocatechin-3-Gallate Improves Acne in Humans by Modulating Intracellular Molecular Targets and Inhibiting P. acnes

Yoon

Kwon

Min

et al. 2013

Journal of Investigative Dermatology

132

106

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“…54 Akt is also considered an important regulator of several signaling pathways that can lead to autoimmunity. 55 There have been mixed reports regarding the effects of EGCG on Akt depending on the cell type studied, as EGCG is shown to reduce Akt phosphorylation in endothelial cells, tumor associated endothelial cells and caco-2 cells, [56][57][58][59] but activate Akt in lung carcinoma cells and osteoblasts. 60,61 Here, we show that EGCG reduces Akt activation and subsequently inflammation in immune stimulated mesangial cells.…”

Section: Egcg Inhibits Inflammation In Lupus Mesangial Cellsmentioning

confidence: 99%

Epigallocatechin-3-gallate (EGCG) attenuates inflammation in MRL/lpr mouse mesangial cells

et al. 2010

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Epigallocatechin-3-gallate (EGCG), a bioactive component of green tea, has been reported to exert anti-inflammatory effects on immune cells. EGCG is also shown to activate the metabolic regulator, adenosine 5'-monophosphate-activated protein kinase (AMPK). Reports have also indicated that EGCG inhibits the immune-stimulated phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway. The PI3K/Akt/mTOR pathway has been implicated in mesangial cell activation in lupus. Mesangial cells from MRL/lpr lupus-like mice are hyper-responsive to immune stimulation and overproduce nitric oxide (NO) and other inflammatory mediators when stimulated. In our current studies, we sought to determine the mechanism by which EGCG attenuates immune-induced expression of pro-inflammatory mediators. Cultured mesangial cells from MRL/lpr mice were pre-treated with various concentrations of EGCG and stimulated with lipopolysaccharide (LPS)/interferon (IFN)-c. EGCG activated AMPK and blocked LPS/ IFN-c-induced inflammatory mediator production (iNOS expression, supernatant NO and interleukin-6). Interestingly, EGCG attenuated inflammation during AMPK inhibition indicating that the anti-inflammatory effect of EGCG may be partially independent of AMPK activation. Furthermore, we found that EGCG effectively inhibited the immune-stimulated PI3K/Akt/mTOR pathway independently of AMPK, by decreasing phosphorylation of Akt, suggesting an alternate mechanism for EGCG-mediated anti-inflammatory action in mesangial cells. Taken together, these studies show that EGCG attenuated inflammation in MRL/lpr mouse mesangial cells via the PI3K/Akt/mTOR pathway. Our findings suggest a potential therapeutic role for the use of EGCG to regulate inflammation and control autoimmune disease.

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“…These phenolic compounds, including anthocyanin, proanthocyanidin (cathechins) and condensed tannin, may be the source of putative health benefits derived from processed goods such as wine (6)(7)(8). A wide range of polyphenolic constituents have been reported to exhibit anticancer and anti-inflammatory effects, as well as the ability to block cellular events predisposing to atherosclerosis and coronary heart disease (CHD) (9)(10)(11)(12)(13). In recent years, resveratrol has been shown to exhibit several biological activities, including cardioprotection and antioxidant activities, inhibition of platelet aggregation, and anti-inflammatory activity (14)(15)(16)(17)(18).…”

mentioning

confidence: 99%

Inhibition of Low-Density Lipoprotein Oxidation by Nagano Purple Grape (Vitis vinifera×Vitis labrusca)

Kamiyama¹,

Kishimoto²,

Tani³

et al. 2009

J Nutr Sci Vitaminol

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SummaryThe Nagano Purple grape ( Vitis ( V. ) vinifera ϫ V. labrusca ) is a hybrid created by a cross between Kyoho ( V. vinifera ϫ V. labrusca ) and Rosario Bianco ( V. vinifera ) grapes. The grape, including its skin, can be eaten and contains no seeds because of gibberellin treatment. The skins of various fruits have been shown to contain antioxidant activity. However, it is unclear whether the Nagano Purple grape contains antioxidant activity. We prepared the skins and dried fruits (including the skins) of the Nagano Purple grape, so as to assay for the presence of an antioxidant activity. We examined the concentration of polyphenols in the grape and further assayed whether components in the grape inhibited the oxidation of low-density lipoprotein (LDL). We detected the presence of cyanidin-3-glucoside (Cy-3-glc), five anthocyanidins and resveratrol in the skins. A trace of resveratrol was detected in the pulp. LDL collected from human subjects 1 h following the consumption of the skins or dried fruits revealed significant inhibition of LDL oxidation compared to that observed in fasting venous blood samples. We further observed the antioxidant activity of Cy-3-glc. Our results suggest that the consumption of the Nagano Purple grape can give rise to resistance to LDL oxidation.

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The anti-inflammatory and anti-cancer properties of epigallocatechin-3-gallate are mediated by folate cycle disruption, adenosine release and NF-κB suppression

Abstract: The data suggest that by modulating NF-kappaB activation, EGCG might not only combat inflammation, but also cancer.

Cited by 48 publications

References 36 publications

Epigallocatechin-3-Gallate Improves Acne in Humans by Modulating Intracellular Molecular Targets and Inhibiting P. acnes

Epigallocatechin-3-Gallate Improves Acne in Humans by Modulating Intracellular Molecular Targets and Inhibiting P. acnes

Epigallocatechin-3-gallate (EGCG) attenuates inflammation in MRL/lpr mouse mesangial cells

Inhibition of Low-Density Lipoprotein Oxidation by Nagano Purple Grape (Vitis vinifera×Vitis labrusca)

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