The anti-inflammatory and analgesic activities of the ethyl acetate extract of Viburnum taitoense Hayata

Wu, Xinduo; Xie, Jizhao; Qiu, Li; Zou, Luhui; Huang, Yanyan; Xie, Yunfei; Xu, Huanji; He, Shineng; Zhang, Qing

doi:10.1016/j.jep.2020.113742

Cited by 20 publications

(15 citation statements)

References 25 publications

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“…All these elements stimulate the development of inflammation and worsen the inflammation over the harmonious interaction with further inflammatory intermediaries and there are reports that in variety of inflammatory ailments including asthma and colitis the expression levels of PGE2 and NO is elevated [15–17]. Our study revealed that Viphyllin efficiently inhibited the secretion of PGE2 and NO, and proinflammatory cytokines in LPS-challenged macrophages at safer dose, our findings are consistent with earlier published studies [18, 19]. COX-2.…”

Section: Discussionsupporting

confidence: 92%

Anti-inflammatory and neuroprotective activity of Viphyllin a standardized extract of β-caryophyllene from black pepper (Piper Nigrum L) and its associated mechanisms in mouse macrophage cells and Human Neuroblastoma SH-SY5Y cells

Gouthamchandra¹,

Venkataramana²,

Sathish³

et al. 2021

Preprint

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Oxidative stress breeds various chronic lifestyle ailments including inflammatory conditions and neurodegenerative diseases. β-caryophyllene natural bicyclic sesquiterpene, obtained from various plants sources found to be effective against inflammation and neuroprotection. In this study, we have evaluated the protective effect of Viphyllin, a standardized extract of β-caryophyllene from black pepper against inflammation induced by lipopolysaccharide in RAW264.7 macrophage cells and mechanisms involved in hydrogen peroxide (H2O2)-challenged oxidative stress in human neuroblastoma SH-SY5Y cells. Viphyllin demonstrated the anti-inflammatory activity by subsiding the release of the pro-inflammatory intermediaries like NO, cytokines, interleukins, and protein expression levels of cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS). In addition, Viphyllin suppressed the extracellular signal-regulated kinase (ERK). c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) phosphorylation. On the other hand, Viphyllin showed neuroprotective effect against neuronal oxidative damage caused by H2O2. Viphyllin lessened the expression B-cell lymphoma-2 (Bcl-2), B-cell lymphoma-2-associated X protein (BAX), cleaved caspase-9, and PARP-1 proteins associated with apoptosis. Our results indicate that Viphyllin ameliorated LPS-mediated inflammation in macrophages by regulating inflammation and Viphyllin exerted remarkable anti apoptotic effect against neuronal damage challenged by H2O2. Altogether, Viphyllin could be potential functional food ingredient for inflammation and neurodegenerative diseases.

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Section: Discussionsupporting

confidence: 92%

Anti-inflammatory and neuroprotective activity of Viphyllin a standardized extract of β-caryophyllene from black pepper (Piper Nigrum L) and its associated mechanisms in mouse macrophage cells and Human Neuroblastoma SH-SY5Y cells

Gouthamchandra¹,

Venkataramana²,

Sathish³

et al. 2021

Preprint

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“…These ndings suggest the presence of free hydroxyl groups is needed for optimum activity. It was revealed that the triterpenoids exhibited better anti-in ammatory activity, which was found to agree with previous reports (Ou et al, 2019;Wu et al, 2021).…”

Section: Gene Expression Studysupporting

confidence: 92%

“…Carrageenan-induced in ammation in the paw mouse model revealed a similar pattern of NO production and expression of pro-in ammatory cytokines, including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumour necrosis factor-α (TNF-α) (Ahn et al, 2020). Various traditionally used herbal extracts were found to be a source of triterpenoids, carrying anti-in ammatory activity (Ou et al, 2019;Wu et al, 2021). Similar to the previous reports, the current study demonstrated signi cantly reduced IL-6, IL-1β, and TNF-α mRNA levels in tissue homogenates of in amed paw tissues with anti-in ammatory leaf extract activity.…”

Section: Gene Expression Studysupporting

confidence: 88%

In vitro and in vivo anti-inflammatory activity of Tetrastigma sulcatum leaf extract, pure compound and its derivatives

et al. 2022

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The severity and perseverance of the in ammation have been demonstrated in many health conditions.The limitations of existing medications, propose the need for newer alternative anti-in ammatory medications. In our earlier studies, we demonstrated the topical anti-in ammatory potential of crude ethanolic extract of Tetrastigma sulcatum leaves and its fractions. In the present study, we further explored anti-in ammatory activity of T. sulcatum extract, fractions, pure compound and its derivatives using in vitro and in vivo bioassay techniques. We attempted to isolate a pure compound from leaf extract and was identi ed as Friedelan-3β-ol (CI) and its derivatives Friedelinol acetate (C II) and Friedelinol methyl ether (C III) were synthesized. Treatment with crude extract and its fractions demonstrated a signi cant reduction in the mRNA expression levels of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) and nitric oxide (NO) production in LPS-stimulated in ammation in RAW 264.7 cells.Likewise, compounds CI, CII and CIII showed a similar pattern of signi cant inhibition of proin ammatory cytokines and NO production. In vivo study in Carrageenan induced paw-in ammatory mice model demonstrated reduced paw oedema and proin ammatory cytokines levels in a dose-dependent manner upon treatment of extract, its fractions, pure compound (CI), and their derivatives (CII and CIII.). The docking study showed all the compounds (CI, CII and CIII) share common residues with Dexamethasone. TNF-α exhibited the most interacting residues with the compounds. The present study con rmed the T. sulcatum 's anti-in ammatory activity, suggesting Friedelan-3β-ol as an active component in a crude extract.

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“…Since many analgesic drugs exert anti-inflammatory activity simultaneously [36], the anti-inflammatory potential of polyalthic acid was presently evaluated with the carrageenan model, finding a significant reduction in paw edema. This effect may be related to the nitric oxide pathway apparently involved in the antinociceptive mechanism.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Antinociceptive, Antiallodynic, Antihyperalgesic and Anti-Inflammatory Effect of Polyalthic Acid

et al. 2021

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Nonsteroidal anti-inflammatory drugs (NSAIDs) are very commonly used, but their adverse effects warrant investigating new therapeutic alternatives. Polyalthic acid, a labdane-type diterpenoid, is known to produce gastroprotection, tracheal smooth muscle relaxation, and antitumoral, antiparasitic and antibacterial activity. This study aimed to evaluate the antinociceptive, antiallodynic, antihyperalgesic and anti-inflammatory effect of polyalthic acid on rats. Moreover, the effectiveness of treating hyperalgesia with a combination of polyalthic acid and naproxen was analyzed, as well as the type of drug–drug interaction involved. Nociception was examined by injecting 1% formalin into the right hind paw and thermal hyperalgesia and inflammation by injecting a 1% carrageenan solution into the left hind paw of rats. Allodynia was assessed on an L5/L6 spinal nerve ligation model. Polyalthic acid generated significant antinociceptive (56–320 mg/kg), antiallodynic (100–562 mg/kg), and antihyperalgesic and anti-inflammatory (10–178 mg/kg) effects. Antinociception mechanisms were explored by pretreating the rats with naltrexone, ODQ and methiothepin, finding the effect blocked by the former two compounds, which indicates the participation of opioid receptors and guanylate cyclase. An isobolographic analysis suggests synergism between polyalthic acid and naproxen in the combined treatment of hyperalgesia.

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The anti-inflammatory and analgesic activities of the ethyl acetate extract of Viburnum taitoense Hayata

Cited by 20 publications

References 25 publications

Anti-inflammatory and neuroprotective activity of Viphyllin a standardized extract of β-caryophyllene from black pepper (Piper Nigrum L) and its associated mechanisms in mouse macrophage cells and Human Neuroblastoma SH-SY5Y cells

Anti-inflammatory and neuroprotective activity of Viphyllin a standardized extract of β-caryophyllene from black pepper (Piper Nigrum L) and its associated mechanisms in mouse macrophage cells and Human Neuroblastoma SH-SY5Y cells

In vitro and in vivo anti-inflammatory activity of Tetrastigma sulcatum leaf extract, pure compound and its derivatives

Evaluation of the Antinociceptive, Antiallodynic, Antihyperalgesic and Anti-Inflammatory Effect of Polyalthic Acid

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