The Anti-Immobility Effect of Hyperoside on the Forced Swimming Test in Rats is Mediated by the D2-Like Receptors Activation

Abstract

The crude extracts of HYPERICUM species native to South Brazil showed analgesic and antidepressant-like effects in rodents. The chemical characterization of these species revealed that they are rich in flavonoids and phloroglucinol derivatives. In the present study a detailed investigation was performed on the activities of hyperoside (HYP), a common flavonoid in the genus HYPERICUM. Hyperoside was obtained from the aerial parts of H. CAPRIFOLIATUM by chromatographic procedures. Mice treated with single doses (10, 20 and 40 mg/kg i.p.) did not present signs of toxicity or weight loss. At 20 and 40 mg/kg i.p. the mice exploratory behavior in the open field test was reduced. At 20 mg/kg i. p. the pentobarbital sleeping time increased, but not the sleeping latency. No activity was found on the hot-plate (10 and 20 mg/kg i.p.) or in the acetic acid-induced writhing test (20 and 40 mg/kg p.o.). Nevertheless, an antidepressant-like effect in the forced swimming test in mice and rats was observed (HYP 10 and 20 mg/kg i.p. in mice; HYP 1.8 mg/kg/day p.o. in rats). The antidepressant-like effect in rats was prevented by the administration of sulpiride (50 mg/kg i.p.) a D2 antagonist. In conclusion, hyperoside was found to present a depressor effect on the central nervous system as well as an antidepressant-like effect in rodents which is, at least in part, mediated by the dopaminergic system.

Antiosteoporotic compounds from seeds of Cuscuta chinensis

“…The effect of quercetin on osteoblasts is controversial, as it has stimulatory or inhibitory effect on osteoblasts differentiation (Notoya et al, 2004;Prouillet et al, 2004) dependent on the origin of osteoblasts, which may explain why we did not observe the effect of quercetin on UMR-106 cells. Hyperoside has been reported to have multiple functions, including anti-immobility, antidepressant, anti-apoptosis and antifungal (Butterweck et al, 2000;Zhang et al, 2010b;Li et al, 2005;Schulte Hass et al, 2010). Here, we show that hyperoside also has stimulatory effect on the ALP activity of osteoblasts, indicating its potential effect on anti-osteoporosis.…”

Induction of Nur77 by hyperoside inhibits vascular smooth muscle cell proliferation and neointimal formation

“…Hyperoside (40 mg/kg) or vehicle was given to mice every 2 days by intraperitoneal injections. The dose of 40 mg/kg hyperoside was chosen based on a previous study [36], and did not show any signs of toxicity or weight loss during the entire study. 14 days after surgery, no neointimal formation was observed in the unligated left common carotid arteries.…”

Antidepressant Flavonoids and Their Relationship with Oxidative Stress

“…Zheng et al [ 61 ] reported that hyperoside possesses antidepressant effects via cytoprotective action related to the elevation of the expression of BDNF and CREB through the signal pathway AC-cAMP-CREB within the PC12 cell line. In addition, Haas et al [ 62 ] concluded that this flavonoid heteroside, extracted from the crude extract of Hypericum caprifoliatum Cham. & Schltdl.…”