The anti-hypoxia effect of Ginsenosides-Rbl on hypoxia cardiomyocytes mediated by the apelin-APJ system ex vivo

Abstract

Objective On the basis of hypoxia induced by CoCl 2 , to elucidate whether Ginsenosides-Rbl (Gs-Rb1) inhibited the apoptosis of hypoxia cardiomyocytes by up-regulating Apelin-APJ system and whether the system was adjusted by Hif-1α. Materials and Methods Neonatal rat cardiomyocytes were randomly divided into control group, simple CoCl 2 group, simple Gs-Rb1 group, CoCl 2 and Gs-Rb1 hypoxia group, CoCl 2 and YC-1 group, CoCl 2 and YC-1 and Gs-Rbl group; The concentration of CoCl 2 , Gs-Rb1 and YC-1 is 500 uM, 500 uM and 5 umol/l, respectively; Apoptosis ratio (AR) was analysed by fl ow cytometer (FCM, Annexin V FITC/PI), and Hif-1α, Apelin and APJ were assayed by immunocytochemistry, Rt-PCR and Western blot. Results (1) Gs-Rb1 signifi cantly down-graded AR of hypoxia cardiomyocytes, which was signifi cantly inhibited by YC-1; (2) Gs-Rb1 signifi cantly increased the expression of hypoxia-induced factor 1α (Hif-1α), which was completely suppressed by YC-1; 3. Hypoxia signifi cantly upgraded the expression of both mRNA and protein of Apelin, which were further reinforced by Gs-Rb1 and signifi cantly inhibited by YC-1; 4. Gs-Rb1 further strengthen the expression of both mRNA and protein of APJ once hypoxia, which was signifi cantly inhibited by YC-1; 5. There was a signifi cant positive correlation between the AR and Apelin (including mRNA and protein), between the AR and APJ (including mRNA and protein). Conclusion Apelin-APJ system, which was adjusted by Hif-1α, plays an important role in Gs-Rb1 anti-hypoxia apoptosis of cardiomyocytes.