“The anti-apoptotic effect ofLonomia obliquahemolymph is associated with the mitochondria pathway”

Abstract

The apoptosis death is a very important factors in production processes that limited the industrial production of some proteins of economic interest. However, one of the forms to increase the cellular productivity would be inhibit or attenuate the cellular death. Recently we have demonstrated the presence of a potent anti-apoptotic protein in Lonomia obliqua hemolymph which extends the cell culture viability through apoptosis prevention. By the other side, has been reported that mitochondria have one important action in the apoptosis control process, being that mitochondria membrane permeabilization (MMP) can be an important stage in this process. MMP associated or not with the loss of the electrochemical potential of the mitochondria and alteration of the matrix is responsible for the inter membranes protein release (e.g. cytochrome c, the AIF, etc) of cytosol. The result obtained showed that the addition of a protein from Lonomia obliqua hemolymph in the culture lead to a prolongation of the cellular life (3-4 days) and the cells leading a high electrochemical potential of the mitochondria. This protein can has its action in mitochondria membrane, avoiding the loss of the membrane permeability and the Cytochrome-C release. As positive control, apoptosis death in these cultures was induced by 50 μm of t-BHP or 600 μm of H202. The presence of apoptosis was characterized by flow citometry, microscopy electronic and agarose gel electrophoresis. The potential electrochemical of the mitochondria was determined by JC-1, Hoechst 33324 and DIOC6. Cytochrome C was identified in cytosol by an anti-cytochrome antibody.

Proteins with anti-apoptotic action in the hemolymph of caterpillars of the Megalopygidae family acts by maintaining the structure of the cellular cytoskeleton

“…high medical and biotechnological interest. Studies with an anti-apoptotic protein isolated from the hemolymph of Lonomia obliqua showed that its likely action would be on the mitochondrial membrane, keeping the electrical potential high and preventing membrane permeability (Mendonça & Martins, 2022).…”

“…We have shown that supplementation of insect and mammalian cell cultures with Lonomia obliqua hemolymph can extend culture viability by preventing death by apoptosis (Maranga et al, 2003; Raffoul et al, 2005; Souza et al, 2005). Recently we have observed that this antiapoptotic effect of hemolymph is associated with the mitochondria pathway, acting in the mitochondria membrane permeabilization (MMP) and with the maintenance of the electrochemical potential of the mitochondria (Mendonça and Martins, 2022). These information makes hemolymph an interesting source of additives for cell cultures.…”