The Anti-Allergic Effects of Lactic Acid Bacteria Are Strain Dependent and Mediated by Effects on both Th1/Th2 Cytokine Expression and Balance

Fujiwara, Daisuke; Inoue, Sayo; Wakabayashi, Hideyuki; Fujii, Toshio

doi:10.1159/000081305

Cited by 231 publications

(232 citation statements)

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“…First, heat-killed cells of these strains were examined for in vitro production of IL-12, IFN-γ and IL-4 by splenocytes from OVA-immunized Balb/c mice, in order to select the strain with strong potential to modulate the Th1/ Th2 balance toward the Th1-dominant state. It has been reported that different Lactobacillus strains, even within the same species, show different patterns of IL-4 and IL-12 production (Fujiwara et al, 2004;Torii et al, 2007;Sakaguchi et al, 2008). In accordance with previous studies, our results strongest activity to suppress IL-4 production.…”

Section: Discussionsupporting

confidence: 93%

“…Certain Lactobacillus strains can potentially modulate immune response through the production of interleukin-12 (IL-12), a potent stimulus for interferon-γ (IFN-γ) production, by antigen-presenting cells, such as dendritic cells (DCs) and macrophages, leading the population of type 1 helper T (Th1) cells and Th2 cells toward a Th1-biased state (Murosaki et al, 1998;Shida et al, 1998;Fujiwara et al, 2004). This effect might be of use in the prevention and treatment of allergic diseases.…”

Section: Introductionmentioning

confidence: 99%

“…posite role to IL-4, and itself promotes Th1 differentiation. Therefore, Lactobacillus strains with the ability to induce a Th1-biased state via IL-12 production, whereby Th2 responses are weakened, are expected to prevent IgE-mediated allergic diseases (Fujiwara et al, 2004;Shida et al, 2002;Cross et al, 2002;Pochard et al, 2002). Although probiotic microbes are recommended to have the ability to survive in the intestine, it has been reported that even heat-killed cells of several Lactobacillus strains exhibit immunomodulatory activity in vivo (Simakachorn et al, 2000;Ishida et al, 2005;Hirose et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Lactobacillus paracasei K71 Isolated from sakekasu (Sake Lees) Suppresses Serum IgE Levels in Ovalbumin-immunized Balb/c Mice

Kumagai¹,

Iguchi²,

Shigeyama

et al. 2013

FSTR

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We investigated 29 Lactobacillus strains isolated from Japanese traditional fermented food products made from rice grain or bran in order to select strains that potently suppress IgE production. First, in vitro screening was carried out to select candidates with strong induction activity for IL-12 and INF-γ production and suppression activity for IL-4 production in mouse splenocytes. Based on the screening data, 3 strains were selected and orally administrated to ovalbumin (OVA)-immunized mice. Among these 3 strains, Lactobacillus paracasei K71 (L. paracasei K71) isolated from sakekasu (sake lees) was shown to be the most potent for decreasing total and OVA-specific IgE levels in serum. L. paracasei K71 may downregulate IgE production via the induction of Th1 responses and suppression of Th2 responses in vivo.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lactobacillus paracasei K71 Isolated from sakekasu (Sake Lees) Suppresses Serum IgE Levels in Ovalbumin-immunized Balb/c Mice

Kumagai¹,

Iguchi²,

Shigeyama

et al. 2013

FSTR

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“…13,[24][25][26] In this study, 117 strains isolated from fermented plant products were screened for strong inducers of IL-12 production in vitro.…”

Section: Discussionmentioning

confidence: 99%

Study of the Relationship between Changes in Lactic Acid Bacterial Cell Components and Stimulation of IL-12 Production under Salt-Stressed Conditions

Igarashi

2010

Bioscience, Biotechnology, and Biochemistry

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One hundred and seventeen strains of plant origin lactic acid bacteria were observed to have interleukin (IL)-12 production-inducing activities using mouse peritoneal macrophages. Pediococcus pentosaceus (KKM122) was chosen for its stable and strong IL-12 production-inducing activity. There was no significant difference in IL-12 activity induced by the KKM122 strain grown in culture conditions of 0% and 6% NaCl. The cell wall components of cells grown in 6% salt condition, however, significantly induced lower IL-12 production as compared with those of cells grown in 0% salt condition. Cell wall components enhanced IL-12 activity by removing cytoplasmic components when KKM122 strain was cultured in 0% salt condition. The immunoenhancing factor was mainly present in the cell wall components. IL-12 production-inducing activities were dependent on both the amount of bacterial cytoplasmic components and the structure of the cell wall components under the NaCl concentration in the culture medium.

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“…They were given a CRF-1 diet (Oriental Yeast, Tokyo, Japan) and water ad libitum for at least 1 week before the experiments. Experiments were performed according to the Guidelines for the Care and Use of Experimental Animals of the Japanese Association for Laboratory Animals.Reagents The reagents used in the experiments include OVA (Sigma, St. Louis, MO, USA), alum (LSL Co., Tokyo, Japan) and B. pertussis (Sigma).Measurement of ovalbumin-induced cytokines and ovalbumin-specific IgE The effects of oral administration of S. crispa on the antigen-induced production of cytokines and the antigen-specific IgE were investigated using a culture system with OVA-stimulated splenocytes derived from mice fed S. crispa as described by Fujiwara et al (2004) with some modifications. BALB/c mice (n = 8 per group) were intraperitoneally sensitized with 20 μg of OVA absorbed onto 2 mg of alum in 0.1 ml sterile saline.…”

mentioning

confidence: 99%

Effects of Hanabiratake (Sparassis crispa) on Allergic Rhinitis in OVA-Sensitized Mice

Yao

Yamamoto

Kimura

et al. 2008

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The anti-rhinitis properties of Sparassis crispa were investigated in mice. To examine the immunomodulative activity of oral administration of S. crispa, splenocytes obtained from ovalbumin-sensitized BALB/ c mice fed S. crispa were restimulated in vitro with the same antigen. Oral administration of S. crispa induced IFN-γ, but inhibited IL-4 and IL-5 secretion, and suppressed ovalbumin-specific IgE secretion by ovalbumin-stimulated splenocytes. The effects of S. crispa were further investigated by using the allergic rhinitis model in BALB/c mice. Nasal symptoms, sneezing and nasal rubbing induced by ovalbumin challenges were inhibited by oral administration of S. crispa in a dose-dependent manner. Furthermore, ovalbumin-specific serum IgE levels were diminished in this model. These results demonstrated that S. crispa may be effective in suppressing symptoms of allergic rhinitis through its immunomodulating activities.Keywords: allergic rhinitis, cytokines, hanabiratake, ovalbumin-specific IgE, Sparassis crispa *To whom correspondence should be addressed. Email: takashi-kimura@unitika.co.jp IntroductionType I allergies, including pollinosis, allergic rhinitis, atopic dermatitis and asthma, are characterized by an elevated production of IgE and mast cell degranulation that result in release of histamine as well as other chemical mediators of allergy (Platts-Mills, 2001). The production of IgE from B cells is regulated by T-helper (Th) cells, which have been classified into Th1 and Th2 subtype (Mosmann et al., 1986). Th2 cells synthesize IL-4, which enhances the IgE production by B cells through inducing IgE isotype class switching and the proliferation of Th2 cells (Le et al., 1990;Swain et al., 1990). Th2 cells also synthesize IL-5 which enhances the IL-4-dependent IgE production (Pene et al., 1988). Therefore, Th2 cells and IL-4 are considered to be critical for IgE production. Conversely, IFN-γ produced by Th1 cells suppresses IgE production both by interfering with the IL-4-derived isotype class switching (Thyphronitis et al., 1989) and by inhibiting the proliferation of Th2 cells (Gajewski and Fitch, 1988). IL-12 produced by antigen-presenting cells, such as dendritic cells and macrophages, is known to stimulate both NK and Th1 cells to make them produce IFN-γ. These two Th1-type cytokines, IL-12 and IFN-γ, enhance the proliferation of Th1 cells (Belosevic et al., 1989;Hsieh et al., 1993). It is generally accepted that enhancement of Th2-mediated immunity causes IgE-dependent allergic diseases. Therefore, properly regulating the balance between Th1-and Th2-type immune responses to heterogeneous antigens is considered to be an important mechanism in the prevention and therapy of the diseases mentioned earlier. In fact, it has been indicated that oral administration of some lactobacillus can modulate the host Th1/Th2 balance and decrease serum IgE levels (Fujiwara et al., 2004;Kimoto et al., 2004). Furthermore, oral administration of the extract from Hatakeshimeji (Lyophyllum decastes) mushroom can modulate t...

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The Anti-Allergic Effects of Lactic Acid Bacteria Are Strain Dependent and Mediated by Effects on both Th1/Th2 Cytokine Expression and Balance

Cited by 231 publications

References 50 publications

Lactobacillus paracasei K71 Isolated from sakekasu (Sake Lees) Suppresses Serum IgE Levels in Ovalbumin-immunized Balb/c Mice

Lactobacillus paracasei K71 Isolated from sakekasu (Sake Lees) Suppresses Serum IgE Levels in Ovalbumin-immunized Balb/c Mice

Study of the Relationship between Changes in Lactic Acid Bacterial Cell Components and Stimulation of IL-12 Production under Salt-Stressed Conditions

Effects of Hanabiratake (Sparassis crispa) on Allergic Rhinitis in OVA-Sensitized Mice

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