The anti-adipogenic effects of (-)epigallocatechin gallate are dependent on the WNT/β-catenin pathway

Lee, Haeyong; Bae, Sung Jin; Yoon, Yoosik

doi:10.1016/j.jnutbio.2012.09.007

Cited by 71 publications

(44 citation statements)

References 44 publications

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“…The same membrane was stripped using a stripping buffer for 30 min, and blocked and probed with a new primary antibody to detect the next specific protein. Band intensities were quantified by ImageJ software (National health institute, USA) (Lee et al, 2013). …”

Section: Methodsmentioning

confidence: 99%

Anti-adipogenic Effects and Mechanisms of Ginsenoside Rg3 in Pre-adipocytes and Obese Mice

Zhang

Wang

et al. 2017

Front. Pharmacol.

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Red or black ginseng has been reported more powerful than white/fresh ginseng in dealing with various diseases/conditions including obesity. The major reason is that heating/steaming, the process of making red or black ginseng, produces large amount of bioactive compounds including ginsenoside Rg3 (Rg3), which are trace in fresh or white ginseng. In the present study, Rg3 was applied both in pre-adipocytes and obese mice to investigate the anti-adipogenic effects and relevant mechanisms. Our results show that Rg3 dose-dependently inhibited cell differentiation both in 3T3-L1 cells (30, 50, and 100 μM) and human primary pre-adipocytes (10, 20, and 30 μM). This inhibitory effect is accompanied by the attenuation of the expressions of adipogenic markers including peroxisome proliferator-activated receptor gamma (PPAR-γ), CCAAT/enhancer binding protein alpha (C/EBP-α), fatty acid synthase (FAS), fatty acid binding protein 4 (FABP4) and perilipin. Although dietary intake of Rg3 (0.1 mg Rg3/kg diet, 8 weeks) did not significantly affect body weight gain, fat pads and food intake as well as of PPAR-γ expression in fat tissues, we found that hepatic PPAR-γ and C/EBP-α protein expressions and hepatic glutathione reductase and glutathione S-transferase, two major antioxidants molecules were significantly reduced by Rg3. These results suggest that ginsenoside Rg3 may be a potential agent in reducing/preventing obesity.

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Section: Methodsmentioning

confidence: 99%

Anti-adipogenic Effects and Mechanisms of Ginsenoside Rg3 in Pre-adipocytes and Obese Mice

Zhang

Wang

et al. 2017

Front. Pharmacol.

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“…EGCG, the most abundant catechin in green tea, inhibits adipogenesis by reducing the expression of PPARG, C/EBPα, FABP4, and fatty acid synthase while increasing the level of β-catenin in the nucleus. Knocking down of β-catenin using siRNA recovers the expression of these adipogenic markers and attenuates the anti-adipogenic effect of EGCG suggesting Wnt/β-catenin pathway as the anti-adipogenic mechanism of EGCG [112]. EGCG has also been shown to increase apoptosis in mature adipocytes without affecting viability of preadipocytes [113].…”

Section: Flavan-3-olsmentioning

confidence: 99%

Differential effects of dietary flavonoids on adipogenesis

et al. 2018

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Propose Obesity is a fast growing epidemic worldwide. During obesity, the increase in adipose tissue mass arise from two different mechanisms, namely, hyperplasia and hypertrophy. Hyperplasia which is the increase in adipocyte number is characteristic of severe obese patients. Recently, there has been much interest in targeting adipogenesis as therapeutic strategy against obesity. Flavonoids have been shown to regulate several pathways and affect a number of molecular targets during specific stages of adipocyte development. Methods Presently, we provide a review of key studies evaluating the effects of dietary flavonoids in different stages of adipocyte development with a particular emphasis on the investigations that explore the underlying mechanisms of action of these compounds in human or animal cell lines as well as animal models. Results Flavonoids have been shown to regulate several pathways and affect a number of molecular targets during specific stages of adipocyte development. Although most of the studies reveal anti-adipogenic effect of flavonoids, some flavonoids demonstrated proadipogenic effect in mesenchymal stem cells or preadipocytes. Conclusion The anti-adipogenic effect of flavonoids is mainly via their effect on regulation of several pathways such as induction of apoptosis, suppression of key adipogenic transcription factors, activation of AMPK and Wnt pathways, inhibition of clonal expansion, and cell-cycle arrest.

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“…The content of catechins is high in GT and they are thought to be the major bioactive components responsible for the biological functions of GT. Catechins can prevent a number of chronic diseases when regularly ingested in the diet, since they present antioxidant, anticarcinogenic, anti-inflammatory, antiatherogenic, antidiabetic, antibacterial and antiviral activities [20][21][22][23][24]. Other proposed health benefits of consuming GT include its protective effect on autoimmune diseases [25], although there is a lack of evidence for the role of GT catechins in lymphocytes from lymph nodes of obese rats.…”

Section: Introductionmentioning

confidence: 99%

Green tea polyphenols change the profile of inflammatory cytokine release from lymphocytes of obese and lean rats and protect against oxidative damage

Molina

Bolin

Otton

2015

International Immunopharmacology

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The anti-adipogenic effects of (-)epigallocatechin gallate are dependent on the WNT/β-catenin pathway

Cited by 71 publications

References 44 publications

Anti-adipogenic Effects and Mechanisms of Ginsenoside Rg3 in Pre-adipocytes and Obese Mice

Anti-adipogenic Effects and Mechanisms of Ginsenoside Rg3 in Pre-adipocytes and Obese Mice

Differential effects of dietary flavonoids on adipogenesis

Green tea polyphenols change the profile of inflammatory cytokine release from lymphocytes of obese and lean rats and protect against oxidative damage

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