The antagonistic effect of<i>Saccharomyces boulardii</i>on<i>Candida albicans</i>filamentation, adhesion and biofilm formation

Krasowska, Anna; Murzyn, Anna; Dyjankiewicz, Agnieszka; Łukaszewicz, Marcin; Dziadkowiec, Dorota

doi:10.1111/j.1567-1364.2009.00559.x

Cited by 53 publications

(51 citation statements)

References 37 publications

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“…boulardii are included in the group of probiotic fungi. This strain exhibits a significant health-promoting activity involving inter alia pathogen binding and prevention of their adhesion to the intestinal epithelium, inhibition of toxin activity of pathogenic bacteria, or binding of these substances to cell wall components, prevention of diarrhea after antibiotherapy, as well as immunostimulation and anti-inflammatory activity [28,53].…”

Section: Introductionmentioning

confidence: 99%

Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source

Bzducha-Wróbel

Kieliszek

Błażejak

2013

Eur Food Res Technol

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The structure of cell wall of yeasts (genus Saccharomyces) is one of the factors that determine their health-promoting properties connected to the presence of b-glucans and mannoprotein. The aim of the study was to determine the influence of glycerol as a carbon source on structural polymers of cell wall (b-glucan and mannoprotein) of probiotic yeasts Saccharomyces cerevisiae var. boulardii and brewer's yeasts S. cerevisiae R9. Significant increase of the percentage of polysaccharide content in the cell wall dry weight of S. cerevisiae R9 brewer's yeasts was noted (in the range of 10-20 %) after cultivation in medium containing glycerol at a concentration of 2-5 % and pH 4.0. The highest content of carbohydrates in probiotic yeasts' cell wall (58 %) was observed after cultivation in medium containing 3 % of glycerol and pH 5.0. The cell wall of probiotic yeasts was characterized by higher content of mannoprotein comparing with cell wall preparation of brewer's yeasts S. cerevisiae R9 composed mainly of b-glucans. After cultivation in mediums with 2 and 3 % of glycerol, the cell of brewer's yeasts contained the highest amount of b(1,3/1,6)-glucan in dry weight of the cell wall (about 36 %). Glycerol at a concentration of 3 and 5 % also intensified mannoprotein biosynthesis in cell wall of S. cerevisiae R9, approximating their content to those noted in the cells of probiotic yeasts (about 29 % (w/w) of dry weight of the cell wall) after cultivation in a medium of pH 5.0 containing 3 % of glycerol.

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Section: Introductionmentioning

confidence: 99%

Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source

Bzducha-Wróbel

Kieliszek

Błażejak

2013

Eur Food Res Technol

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“…Previously, most studies employing S. boulardii have focused on elucidating putative mechanisms of its beneficial properties and its applications as a probiotic (7)(8)(9)(10)(11)(12). Based on the probiotic trait of S. boulardii, this yeast can be potentially used for in-gut production of therapeutic proteins.…”

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confidence: 99%

Metabolic Engineering of Probiotic Saccharomyces boulardii

Liu

Kong

Zhang

et al. 2016

Appl Environ Microbiol

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Saccharomyces boulardii is a probiotic yeast that has been used for promoting gut health as well as preventing diarrheal diseases. This yeast not only exhibits beneficial phenotypes for gut health but also can stay longer in the gut than Saccharomyces cerevisiae. Therefore, S. boulardii is an attractive host for metabolic engineering to produce biomolecules of interest in the gut. However, the lack of auxotrophic strains with defined genetic backgrounds has hampered the use of this strain for metabolic engineering. Here, we report the development of well-defined auxotrophic mutants (leu2, ura3, his3, and trp1) through clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9-based genome editing. The resulting auxotrophic mutants can be used as a host for introducing various genetic perturbations, such as overexpression or deletion of a target gene, using existing genetic tools for S. cerevisiae. We demonstrated the overexpression of a heterologous gene (lacZ), the correct localization of a target protein (red fluorescent protein) into mitochondria by using a protein localization signal, and the introduction of a heterologous metabolic pathway (xylose-assimilating pathway) in the genome of S. boulardii. We further demonstrated that human lysozyme, which is beneficial for human gut health, could be secreted by S. boulardii. Our results suggest that more sophisticated genetic perturbations to improve S. boulardii can be performed without using a drug resistance marker, which is a prerequisite for in vivo applications using engineered S. boulardii.

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“…Although the active compound was not identified, H 2 O 2 and autoinducer 2 were proposed to promote filamentation by impacting the oxidative stress response. Culture supernatants from Lactobacillus rhamnosus GG and the probiotic yeast Saccharomyces boulardii blocked germ tube formation induced in serum-containing medium and in RPMI 1640 medium at 37°C (73,92,101). Butyric acid was shown to mimic the effect of L. rhamnosus GG spent medium, at concentrations well within the physiological range observed in the colon (101,116).…”

Section: Bacterial and Fungal Autoregulatory Moleculesmentioning

confidence: 99%

Modulation of Morphogenesis in Candida albicans by Various Small Molecules

2011

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The pathogenic yeast Candida albicans, a member of the mucosal microbiota, is responsible for a large spectrum of infections, ranging from benign thrush and vulvovaginitis in both healthy and immunocompromised individuals to severe, life-threatening infections in immunocompromised patients. A striking feature of C. albicans is its ability to grow as budding yeast and as filamentous forms, including hyphae and pseudohyphae. The yeast-to-hypha transition contributes to the overall virulence of C. albicans and may even constitute a target for the development of antifungal drugs. Indeed, impairing morphogenesis in C. albicans has been shown to be a means to treat candidiasis. Additionally, a large number of small molecules such as farnesol, fatty acids, rapamycin, geldanamycin, histone deacetylase inhibitors, and cell cycle inhibitors have been reported to modulate the yeast-to-hypha transition in C. albicans. In this minireview, we take a look at molecules that modulate morphogenesis in this pathogenic yeast. When possible, we address experimental findings regarding their mechanisms of action and their therapeutic potential. We discuss whether or not modulating morphogenesis constitutes a strategy to treat Candida infections.

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The antagonistic effect ofSaccharomyces boulardiionCandida albicansfilamentation, adhesion and biofilm formation

Cited by 53 publications

References 37 publications

Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source

Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source

Metabolic Engineering of Probiotic Saccharomyces boulardii

Modulation of Morphogenesis in Candida albicans by Various Small Molecules

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