The Anopheles gambiae tryptophan oxygenase gene expressed from a baculovirus promoter complements Drosophila melanogaster vermilion

Besansky, Nora J.; Mukabayire, O.; Benedict, Mark Q.; Rafferty, Cristina Salazar; Hamm, Diane Mills; McNitt, Louise

doi:10.1016/s0965-1748(97)00040-4

Cited by 13 publications

(7 citation statements)

References 17 publications

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“…Since the early days of transposon-mediated germline transformation in D. melanogaster, eye-color genes have been the gold standard for transformation markers (RUBIN AND SPRADLING 1982;WHITE et al 1996;BESANSKY et al 1997;SUMITANI et al 2005). Indeed, even with the advent of fluorescent markers, eye-specific expression has been favored over other promoters, via the development of the 3xP3 artificial eye-specific promoter, since it is typically easy to identify (BERGHAMMER et al 1999;HORN et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Window to the Fluorescence: TheWhiteEye-Color Gene of Western Corn Rootworm,Diabrotica virgifera virgifera

Grubbs

Chu

Lorenzen

2019

Preprint

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Eye-color mutations have proven useful in multiple insect species to help facilitate the development and use of transgenic tools for functional genomics. While there is species-specific variation in the pigments used to color insect eyes, every species studied thus far requires an ortholog of the ABC transporter gene white for proper pigmentation of the eyes. Previously, we generated transgenic western corn rootworm, Diabrotica virgifera virgifera, and found that their wild-type eye color obscured our ability to visualize a fluorescent marker driven by the widely used 3xP3 eye-specific promoter. Therefore, we sought to identify the D. v. virgifera ortholog of white (Dvvw). Here we report the discovery, cloning, and analysis of Dvvw cDNA and promoter.We also utilize RNA interference to knock down Dvvw mRNA in a transgenic strain, thereby demonstrating the utility of eye-color mutations when developing transgenic technologies.

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Section: Introductionmentioning

confidence: 99%

Window to the Fluorescence: TheWhiteEye-Color Gene of Western Corn Rootworm,Diabrotica virgifera virgifera

Grubbs

Chu

Lorenzen

2019

Preprint

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“…This marker rescued adult eye color in a strain of Musca domestica that was homozygous for a mutant tryptophane oxygenase gene (White et al 1996). Besansky et al (1997) placed Anopheles gambiae cDNA encoding tryptophan oxygenase under the control of the constitutive baculovirus promoter, ie-1 . This chimeric construct, expressed transiently in vermilion mutants of D. melanogaster , partially rescued adult eye color (Besansky et al 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Besansky et al (1997) placed Anopheles gambiae cDNA encoding tryptophan oxygenase under the control of the constitutive baculovirus promoter, ie-1 . This chimeric construct, expressed transiently in vermilion mutants of D. melanogaster , partially rescued adult eye color (Besansky et al 1997). Genes such as Bmto may prove invaluable as transformation markers in the future.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Analysis of theTryptophan oxygenaseGene in the Silkworm,Bombyx mori

Liu

Meng

Niu

et al. 2008

Journal of Insect Science

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A Bombyx mori L. (Lepidoptera: Bombycidae) gene encoding tryptophan oxygenase has been molecularly cloned and analyzed. The tryptophan oxygenase cDNA had 1374 nucleotides that encoded a 401 amino acid protein with an estimated molecular mass of 46.47 kDa and a PI of 5.88. RT-PCR analysis showed that the B. mori tryptophan oxygenase gene was transcribed in all examined stages. Tryptophan oxygenase proteins are relatively well conserved among different orders of arthropods.

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“…A potential disadvantage of Tcv ‐based selection is that Vermilion proteins might be phylogenetically restricted in function. However, the D. melanogaster vermilion gene has been shown to rescue a tryptophan oxygenase ‐deficient mutation in the house fly, Musca domestica (White et al ., 1996), and the Anopheles gambiae vermilion gene has been shown to rescue a vermilion mutation in D. melanogaster (Besansky et al ., 1997). This raises the possibility that Tcv could also serve as a transformation marker in other families of beetles, provided the appropriate mutant strains were available.…”

Section: Discussionmentioning

confidence: 99%

Transgene expression from the Tribolium castaneum Polyubiquitin promoter

Lorenzen

Brown

Denell

et al. 2002

Insect Molecular Biology

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The highly conserved Ubiquitin proteins are expressed from genes with strong, constitutively active promoters in many species, making these promoters attractive candidates for use in driving transgene expression. Here we report the cloning and characterization of the Tribolium castaneum Polyubiquitin (TcPUb) gene. We placed the TcPUb promoter upstream of the coding region of the T. castaneum eye-colour gene Tc vermilion (Tcv) and injected this construct into embryos from a Tcv-deficient strain. Transient expression of Tcv during embryogenesis resulted in complete rescue of the larval mutant phenotype. We then incorporated the TcPUb-Tcv chimera into a piggyBac donor. Resulting germline transformants were easily recognized by rescue of eye pigmentation, illustrating the potential of the TcPUb promoter for use in driving transgene expression.

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The Anopheles gambiae tryptophan oxygenase gene expressed from a baculovirus promoter complements Drosophila melanogaster vermilion

Cited by 13 publications

References 17 publications

Window to the Fluorescence: TheWhiteEye-Color Gene of Western Corn Rootworm,Diabrotica virgifera virgifera

Window to the Fluorescence: TheWhiteEye-Color Gene of Western Corn Rootworm,Diabrotica virgifera virgifera

Molecular Cloning and Analysis of theTryptophan oxygenaseGene in the Silkworm,Bombyx mori

Transgene expression from the Tribolium castaneum Polyubiquitin promoter

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