The anion transport system of the red blood cell The role of membrane protein evaluated by the use of ‘probes’

Cabantchik, Z. I.; Knauf, Philip A.; Rothstein, Aser

doi:10.1016/0304-4157(78)90016-3

Cited by 703 publications

(245 citation statements)

References 176 publications

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“…the stilbenes were apparently without effect on this system. In the red blood cell the membrane permeability for Cl-pcl-= 1.49 x lop3 cm/s [3] is much greater than in skeletal muscle where PCI-= 4 x 10s6 cm/s [18]. Our study showed that some of the features of the inhibition of Na+/K+-ATPase are similar to those observed for inhibition of anion transport in red blood cells.…”

Section: Discussionsupporting

confidence: 71%

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The effect of anion channel blockers on enzymatic activity of Na⁺/K⁺‐ATPase and the electrogenic Na⁺/K⁺ pump

Teisinger¹,

Zemková²,

Vyskočil³

1984

FEBS Letters

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Disulfonic stilbenes which block the anion‐transport in red blood cells were found to inhibit the brain microsomal Na+/K+‐ATPase but not the electrogenic Na+/K+ pump in intact muscle cells. In contrast to the anion‐transport system, the Na+/K+‐ATPase is inhibited by disulfonic stilbenes, apparently from the cytoplasmic side of the membrane. The pathways for anion and active cation transport are thus different but similar groups of sulfhydryl and/or amino acid residues must play an important role in both systems.

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Section: Discussionsupporting

confidence: 71%

“…The disulfonic stilbenes, SITS, DIDS and DNDS which are covalent binding agents for exposed amino groups on proteins [l] block anion transport in red blood cells [2,3]. SITS also has been found to inhibit the microsomal Na+/K+-ATPase of eel electric organ and turtle bladder [4].…”

Section: Methodsmentioning

confidence: 99%

The effect of anion channel blockers on enzymatic activity of Na⁺/K⁺‐ATPase and the electrogenic Na⁺/K⁺ pump

Teisinger¹,

Zemková²,

Vyskočil³

1984

FEBS Letters

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“…J Cereb Blood Flow Metab, Vol, 13, No, 5, 1993 Regulation of an alkaline transient An electroneutral anion transport mechanism, passively transporting CI-in exchange for HC03 -, has been extensively studied in erythrocytes but as also been identified in many other mammalian cells, including cultured rat astrocytes (Cabantchik et al, 1978;Reinertsen et al, 1988;Mason et al, 1989;Mellergard et al, 1993). This exchanger is consid ered as a separate HC03 -transport mechanism, inhibited by stilbene derivatives.…”

Section: Acid Extrusion Mechanismsmentioning

confidence: 99%

Regulation of Intracellular pH in Single Rat Cortical Neurons in vitro: A Microspectrofluorometric Study

Ouyang

Mellergård

Siesjö

1993

J Cereb Blood Flow Metab

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Summary: Intracellular pH (pHi) and the mechanisms of pHi regulation in cultured rat cortical neurons were stud ied with microspectrofluorometry and the pH-sensitive fluorophore 2',7' -bis( carboxyethyl)-5 ,6-carboxyfluore scein. Steady-state pHi was 7.00 ± 0.17 (mean ± SD) and 7.09 ± 0.14 in nominally HC03 --free and HC03 -containing solutions, respectively, and was dependent on extracellular Na + and Cl -. Following an acid transient, induced by an NH] prepulse or an increase in CO2 ten sion, pHi decreased and then rapidly returned to baseline, with an average net acid extrusion rate of 2.6 and 2.8 mmollLlmin, in nominally HC03 --free and HC03 --containing solutions, respectively. The recovery was completely blocked by removal of extracellular Na + and was partially inhibited by amiloride or 5-N-methyl-N-iso butylamiloride. In most cells pHi recovery was com-

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“…Also, band 2.1 and the series of polypeptides related to band 2.1 by sequence homology have been called "syndeins" (34). Band 3 (17) penetrates the lipid bilayer wholly (8,25), which may be controlled by cytoskeletal proteins (7). Steck has reported that band 7 is only one perturbant-resistant component of the cytoplasmic surface (25).…”

Section: Discussionmentioning

confidence: 99%

Studies on Toxin of Aspergillus fumigatus

Ebina

Ichinowatari

Yokota

1985

Microbiology and Immunology

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The fashion of binding of Asp-hemolysin to human erythrocytes and the isolation of Asp-hemolysin-binding proteins from erythrocyte membranes were investigated by the immunocytochemical technique and affinity chromatography. Asp-hemolysin bound best at a pH range from 5 to 7. The erythrocytes treated with Asp-hemolysin showed diffuse, ring-like or cap-like staining by the peroxidaselabeled antibody method under the light microscope. The distribution of Asphemolysin on the erythrocyte surface was clearly observed as patches or caps in the scanning electron microscope. The erythrocyte ghosts were extracted with 1% sodium deoxycholate-0.1 M Tris-HC1 buffer (pH 7.5) containing 0.2 M NaCl and 1 aim EDTA, and the extract was chromatographed on an affinity column consisting of Asp-hemolysin attached to activated thiol-Sepharose 4B. Four proteins present in the membrane extract were retained by activated thiol-Sepharose 4B and eluted with 50 mm cysteine as toxin-membrane components. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated that the polypeptides correspond to band 2.1, one protein of the 2 region, band 3 and band 7 in the Steck nomenclature system.

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The anion transport system of the red blood cell The role of membrane protein evaluated by the use of ‘probes’

Cited by 703 publications

References 176 publications

The effect of anion channel blockers on enzymatic activity of Na⁺/K⁺‐ATPase and the electrogenic Na⁺/K⁺ pump

The effect of anion channel blockers on enzymatic activity of Na⁺/K⁺‐ATPase and the electrogenic Na⁺/K⁺ pump

Regulation of Intracellular pH in Single Rat Cortical Neurons in vitro: A Microspectrofluorometric Study

Studies on Toxin of Aspergillus fumigatus

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The anion transport system of the red blood cell The role of membrane protein evaluated by the use of ‘probes’

Cited by 703 publications

References 176 publications

The effect of anion channel blockers on enzymatic activity of Na+/K+‐ATPase and the electrogenic Na+/K+ pump

The effect of anion channel blockers on enzymatic activity of Na+/K+‐ATPase and the electrogenic Na+/K+ pump

Regulation of Intracellular pH in Single Rat Cortical Neurons in vitro: A Microspectrofluorometric Study

Studies on Toxin of Aspergillus fumigatus

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Product

Resources

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The effect of anion channel blockers on enzymatic activity of Na⁺/K⁺‐ATPase and the electrogenic Na⁺/K⁺ pump

The effect of anion channel blockers on enzymatic activity of Na⁺/K⁺‐ATPase and the electrogenic Na⁺/K⁺ pump